The mixed/mixotrophic nitrogen removal for the effective and sustainable treatment of wastewater: From treatment process to microbial mechanism

Water Research, Год журнала: 2022, Номер 226, С. 119269 - 119269

Опубликована: Окт. 17, 2022

Язык: Английский

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In-depth characterization of denitrifier communities across different soil ecosystems in the tundra

Environmental Microbiome, Год журнала: 2022, Номер 17(1)

Опубликована: Июнь 11, 2022

Abstract Background In contrast to earlier assumptions, there is now mounting evidence for the role of tundra soils as important sources greenhouse gas nitrous oxide (N 2 O). However, microorganisms involved in cycling N O this system remain largely uncharacterized. Since are variable and sinks O, we aimed at investigating differences community structure across different soil ecosystems tundra. Results We analysed 1.4 Tb metagenomic data from northern Finland covering a range dry upland water-logged fens obtained 796 manually binned curated metagenome-assembled genomes (MAGs). then searched MAGs harbouring genes denitrification, an process driving emissions. Communities potential denitrifiers were dominated by with truncated denitrification pathways (i.e., lacking one or more genes) differed ecosystems. Upland showed strong sink members Alphaproteobacteria such Bradyrhizobium Reyranella . Fens, which had general net-zero fluxes, high abundance poorly characterized taxa affiliated Chloroflexota lineage Ellin6529 Acidobacteriota subdivision Gp23. Conclusions By coupling in-depth characterization microbial communities situ measurements our results suggest that observed spatial patterns fluxes related composition denitrifier communities.

Язык: Английский

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microTrait: A Toolset for a Trait-Based Representation of Microbial Genomes

Frontiers in Bioinformatics, Год журнала: 2022, Номер 2

Опубликована: Июль 22, 2022

Remote sensing approaches have revolutionized the study of macroorganisms, allowing theories population and community ecology to be tested across increasingly larger scales without much compromise in resolution biological complexity. In microbial ecology, our remote window into microorganisms is through lens genome sequencing. For organisms, recent evidence from genomes recovered metagenomic samples corroborate a highly complex view their metabolic diversity other associated traits which map high physiological Regardless, during first decades this omics era, ecological research has primarily focused on taxa functional genes as units, favoring breadth coverage over complexity manifested diversity. Recently, rate at provisional draft are generated increased substantially, giving new insights processes interactions. From genotype perspective, wide availability genome-centric data requires synthesis that place organismal center stage environmental roles performance. Extraction ecologically relevant will essential future research. Here, we present microTrait , computational pipeline infers distills sequences. maps sequence trait space, including discrete continuous traits, well simple composite. Traits inferred pathways representing energetic, resource acquisition, stress tolerance mechanisms, while genome-wide signatures used infer composite, or life history, microorganisms. This approach extensible any habitat, although provide initial examples with reference soil microbiomes.

Язык: Английский

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Stress-induced metabolic exchanges between complementary bacterial types underly a dynamic mechanism of inter-species stress resistance

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Май 31, 2023

Metabolic cross-feeding plays vital roles in promoting ecological diversity. While some microbes depend on exchanges of essential nutrients for growth, the forces driving extensive needed to support coexistence free-living are poorly understood. Here we characterize bacterial physiology under self-acidification and establish that excretion key metabolites following growth arrest provides a collaborative, inter-species mechanism stress resistance. This collaboration occurs not only between species isolated from same community, but also unrelated with complementary (glycolytic vs. gluconeogenic) modes metabolism. Cultures such communities progress through distinct phases growth-dilution cycles, comprising exponential acidification-triggered arrest, collaborative deacidification, recovery, each phase involving different combinations physiological states individual species. Our findings challenge steady-state view ecosystems commonly portrayed models, offering an alternative dynamical based advantages phases.

Язык: Английский

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Aerobic bacteria produce nitric oxide via denitrification and promote algal population collapse

The ISME Journal, Год журнала: 2023, Номер 17(8), С. 1167 - 1183

Опубликована: Май 12, 2023

Microbial interactions govern marine biogeochemistry. These are generally considered to rely on exchange of organic molecules. Here we report a novel inorganic route microbial communication, showing that algal-bacterial between Phaeobacter inhibens bacteria and Gephyrocapsa huxleyi algae mediated through nitrogen exchange. Under oxygen-rich conditions, aerobic reduce algal-secreted nitrite nitric oxide (NO) denitrification, well-studied anaerobic respiratory mechanism. The bacterial NO is involved in triggering cascade akin programmed cell death. During death, further generate NO, thereby propagating the signal algal population. Eventually, population collapses, similar sudden demise oceanic blooms. Our study suggests species oxygenated environments potentially significant communication within across kingdoms.

Язык: Английский

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How to Provide Nitrite Robustly for Anaerobic Ammonium Oxidation in Mainstream Nitrogen Removal

Environmental Science & Technology, Год журнала: 2023, Номер 57(51), С. 21503 - 21526

Опубликована: Дек. 14, 2023

Innovation in decarbonizing wastewater treatment is urgent response to global climate change. The practical implementation of anaerobic ammonium oxidation (anammox) treating domestic the key reconciling carbon-neutral management with sustainable development. Nitrite availability prerequisite anammox reaction, but how achieve robust nitrite supply and accumulation for mainstream systems remains elusive. This work presents a state-of-the-art review on recent advances anammox, paying special attention available pathways (forward-going (from nitrite) backward-going nitrate nitrite)), controlling strategies, physiological ecological characteristics functional microorganisms involved supply. First, we comprehensively assessed nitrite-oxidizing bacteria control methods, outlining that these technologies are transitioning possessing multiple selective pressures (such as intermittent aeration membrane-aerated biological reactor), integrating side stream free ammonia/free nitrous acid suppression recirculated sludge treatment), maintaining high activity ammonia-oxidizing competing oxygen bacteria. We then highlight emerging strategies supply, including production driven by novel microbes (ammonia-oxidizing archaea complete ammonia bacteria) reduction (partial denitrification nitrate-dependent methane oxidation). resources requirement different analyzed, hybrid pathway combining partial nitrification encouraged. Moreover, data-driven modeling process well proactive microbiome proposed hope achieving application. Finally, existing challenges further perspectives highlighted, i.e., investigation nitrite-supplying bacteria, scaling-up from laboratory under real conditions, stable performance fundamental insights this aim inspire advance our understanding about provide robustly shed light important obstacles warranting settlement.

Язык: Английский

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Environmentally dependent interactions shape patterns in gene content across natural microbiomes

Nature Microbiology, Год журнала: 2024, Номер 9(8), С. 2022 - 2037

Опубликована: Июль 8, 2024

Язык: Английский

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Exploring Microbial Rhizosphere Communities in Asymptomatic and Symptomatic Apple Trees Using Amplicon Sequencing and Shotgun Metagenomics

Agronomy, Год журнала: 2024, Номер 14(2), С. 357 - 357

Опубликована: Фев. 9, 2024

The rhizosphere is a dynamic and highly interactive habitat where diverse microbial communities are established, it plays crucial roles in plant health disease dynamics. In this study, functional profiles the of both asymptomatic symptomatic apple trees were investigated through amplicon sequencing shotgun metagenomics. research was conducted at location municipality Cuauhtemoc, Chihuahua State, Mexico, total 22 samples collected, comprising 12 for 10 metagenomic sequencing. Symptomatic identified based on reddish branches internal necrosis trunk root, while exhibited healthy physiology. findings showed that dominant bacterial phyla included Proteobacteria, Actinobacteria, Bacteroidetes, with prevalent genera such as Streptomyces, Pseudomonas, Rhodanobacter. fungal featured Ascomycota, Mortierellomycota, Basidiomycota, which dominated by Fusarium, Penicillium, Mortierella. communities, notably abundant trees, holds potential biocontrol agent, seen other studies suppression Fusarium wilt disease. application revealed significant differences alpha beta diversities suggesting health-dependent change species composition abundance. Functional profile analysis highlighted enzymatic activities associated lipid synthesis/degradation, amino acid biosynthesis, carbohydrate metabolism, nucleotide synthesis, have been documented to participate symbiotic relationships between plants. These insights not only contribute understanding dynamics activity but also provide valuable perspectives tree implications management orchards.

Язык: Английский

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The vertical partitioning between denitrification and dissimilatory nitrate reduction to ammonium of coastal mangrove sediment microbiomes

Water Research, Год журнала: 2024, Номер 262, С. 122113 - 122113

Опубликована: Июль 19, 2024

Язык: Английский

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Determining how oxygen legacy affects trajectories of soil denitrifier community dynamics and N2O emissions

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 24, 2024

Denitrification – a key process in the global nitrogen cycle and main source of greenhouse gas N2O is intricately controlled by O2. While transition from aerobic respiration to denitrification well-studied, our understanding denitrifier communities' responses cyclic oxic/anoxic shifts, prevalent natural engineered systems, limited. Here, agricultural soil exposed repeated cycles long or short anoxic spells (LA; SA) constant oxic conditions (Ox). Surprisingly, reduction rates are three times greater Ox than LA SA during final incubation, despite comparable bacterial biomass gene abundances. Metatranscriptomics indicate that favors canonical denitrifiers carrying nosZ clade I. instead II-carrying partial- non-denitrifiers, suggesting efficient partnering steps among organisms. has slowest progression highest accumulation intermediates, indicating less functional coordination. The findings demonstrate how adaptations communities varying O2 tightly linked duration episodes, emphasizing importance knowing an environment's legacy for accurately predicting emissions originating denitrification. A history oxygen exposure enhances under anoxia compared pulses, highlighting accurate emission predictions.

Язык: Английский

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