Frontiers in Bioengineering and Biotechnology,
Год журнала:
2022,
Номер
10
Опубликована: Июль 15, 2022
Filamentous
fungi
are
highly
productive
cell
factories,
many
of
which
industrial
producers
enzymes,
organic
acids,
and
secondary
metabolites.
The
increasing
number
sequenced
fungal
genomes
revealed
a
vast
unexplored
biosynthetic
potential
in
the
form
transcriptionally
silent
metabolite
gene
clusters
(BGCs).
Various
strategies
have
been
carried
out
to
explore
mine
this
untapped
source
bioactive
molecules,
with
advent
synthetic
biology,
novel
applications,
tools
developed
for
filamentous
fungi.
Here
we
summarize
approaches
aiming
expression
endogenous
or
exogenous
natural
product
BGCs,
including
transcription
factors,
assembly
artificial
units,
cluster
refactoring,
shuttle
vectors,
platform
strains.
Cell,
Год журнала:
2019,
Номер
176(4), С. 687 - 701.e5
Опубликована: Фев. 1, 2019
Female
Aedes
aegypti
mosquitoes
bite
humans
to
obtain
blood
develop
their
eggs.
Remarkably,
strong
attraction
is
suppressed
for
days
after
the
meal
by
an
unknown
mechanism.
We
investigated
a
role
neuropeptide
Y
(NPY)-related
signaling
in
long-term
behavioral
suppression
and
discovered
that
drugs
targeting
human
NPY
receptors
modulate
mosquito
host-seeking.
In
screen
of
all
49
predicted
Ae.
peptide
receptors,
we
identified
NPY-like
receptor
7
(NPYLR7)
as
sole
target
these
drugs.
To
small-molecule
agonists
selective
NPYLR7,
performed
high-throughput
cell-based
assay
265,211
compounds
isolated
six
highly
NPYLR7
inhibit
humans.
CRISPR-Cas9
null
mutants
are
defective
resistant
Finally,
show
can
biting
blood-feeding
on
live
host,
suggesting
novel
approach
control
infectious
disease
transmission
controlling
behavior.Video
AbstracteyJraWQiOiI4ZjUxYWNhY2IzYjhiNjNlNzFlYmIzYWFmYTU5NmZmYyIsImFsZyI6IlJTMjU2In0.eyJzdWIiOiJjZGQ0ODkyMTY5YWZhZDcyOWFjMGM2NzZkNTQyZGE1YyIsImtpZCI6IjhmNTFhY2FjYjNiOGI2M2U3MWViYjNhYWZhNTk2ZmZjIiwiZXhwIjoxNjc4NTU0NzMyfQ.IWqIlOv-k_s5VeH8vGctMuo6d2wnVQraplkJ0BqNeSWpc9I1CuK41HdQ7D0Y6zjDHGcYmCbA6N0lcR7GhcwcYlJktBF9CHD5koGyOMgn6PpFoexqUY5L9_rheIMShGWZjfq-W2eu99JcPhHcNOXLQ3nI54um-tcAKtPkF34pXJyOtVpX82kWquECPon_feVDg7YME6Znl4oyzekjKhsJzRBOO9-7XghUE78QjO2glylQt-WovwhdjdTuq8lpd2U5XEezFwsr68oTZaENO_q4uDjfzWNPOlJwE84dhLxMJYbGEIoehrmIgjzRsefINHApB-S0Y68C00Y3CXXoaHvtUw(mp4,
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Cell,
Год журнала:
2023,
Номер
186(14), С. 3079 - 3094.e17
Опубликована: Июнь 15, 2023
Ants
communicate
via
large
arrays
of
pheromones
and
possess
expanded,
highly
complex
olfactory
systems,
with
antennal
lobes
in
the
brain
comprising
up
to
∼500
glomeruli.
This
expansion
implies
that
odors
could
activate
hundreds
glomeruli,
which
would
pose
challenges
for
higher-order
processing.
To
study
this
problem,
we
generated
transgenic
ants
expressing
genetically
encoded
calcium
indicator
GCaMP
sensory
neurons.
Using
two-photon
imaging,
mapped
complete
glomerular
responses
four
ant
alarm
pheromones.
Alarm
robustly
activated
≤6
activity
maps
three
inducing
panic
our
species
converged
on
a
single
glomerulus.
These
results
demonstrate
that,
rather
than
using
broadly
tuned
combinatorial
encoding,
employ
precise,
narrowly
tuned,
stereotyped
representations
The
identification
central
hub
glomerulus
behavior
suggests
simple
neural
architecture
is
sufficient
translate
pheromone
perception
into
behavioral
outputs.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 25, 2025
Central
nervous
system
(CNS)
disorders
confront
significant
challenges
in
drug
delivery
due
to
the
blood-brain
barrier
(BBB).
Inspired
by
rapid
and
precise
binding
of
odor
molecules
olfactory
receptors
(ORs),
this
research
uses
thiolated
HPMA
construct
nanoparticles
(nanoodors)
capable
delivering
drugs
CNS
via
olfacto-cerebral
pathway
overcome
obstruction.
The
nanoodor
core
is
used
encapsulate
agomelatine
(AGO),
a
CNS-targeting
antidepressant,
encapsulation
efficiency
exceeded
80%.
A
series
thiol-presenting
nanoscale
structures
with
different
surface
densities
thiol
groups
are
constructed,
effectiveness
positively
correlated
density
on
their
surface.
Notably,
nanoodors
enable
brain-targeted
delivery,
outperforming
commercially
available
oral
formulations
terms
accumulation
brain
antidepressant
effects.
study
transport
action
mechanisms
revealed
that
after
ORs,
rapidly
delivered
pathway.
Nanoodors,
first
design
deliver
mimicking
natural
smells
for
treatment
disorders,
expected
achieve
clinical
transformation,
benefiting
human
health.
Current Opinion in Insect Science,
Год журнала:
2017,
Номер
20, С. 34 - 38
Опубликована: Март 14, 2017
Mosquitoes
detect
the
presence
of
humans
by
integrating
chemosensory,
thermal,
and
visual
cues.
Among
these,
odors
are
crucial
for
mosquito
host
detection.
Insects
have
evolved
a
diverse
repertoire
receptors
to
their
plant
animal
hosts.
Genetic
analysis
these
in
Drosophila
has
set
stage
similar
studies
mosquitoes.
The
diversity
cues
involved
host-seeking
made
designing
behavioral
control
strategies
challenge.
sensory
that
most
important
detection
can
now
be
determined
using
genome
editing.
Here,
we
will
review
our
current
understanding
salient
attract
mosquitoes,
receptors,
suggest
ways
forward
novel
olfaction-based
vector
strategies.
Journal of Experimental Biology,
Год журнала:
2018,
Номер
221(4)
Опубликована: Фев. 15, 2018
ABSTRACT
Mosquitoes
are
best
known
for
their
proclivity
towards
biting
humans
and
transmitting
bloodborne
pathogens,
but
there
over
3500
species,
including
both
blood-feeding
non-blood-feeding
taxa.
The
diversity
of
host
preference
in
mosquitoes
is
exemplified
by
the
feeding
habits
genus
Malaya
that
feed
on
ant
regurgitation
or
those
from
Uranotaenia
favor
amphibian
hosts.
Host
also
no
means
static,
characterized
behavioral
plasticity
allows
to
switch
hosts
when
preferred
unavailable
learning
cues
associated
with
positive
negative
experiences.
Here
we
review
diverse
range
host-preference
behaviors
across
family
Culicidae,
which
includes
all
mosquitoes,
how
adaptations
neural
circuitry
might
affect
changes
within
life
history
a
mosquito
evolutionary
time-scales.
