Discrete roles of Ir76b ionotropic coreceptor impact olfaction, blood feeding, and mating in the malaria vector mosquito Anopheles coluzzii DOI Creative Commons
Zi Ye,

Feng Liu,

Huahua Sun

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(23)

Published: June 1, 2022

Significance Chemosensory systems play crucial roles across mosquito life cycles, with underlying molecular receptors often forming functional complexes that require cognate coreceptors. To better understand chemosensory pathways in the malaria vector Anopheles coluzzii , gene editing were used to localize and functionally characterize ionotropic receptor coreceptor AcIr76b . Expression of was observed antennal grooved pegs, coeloconic sensilla, other accessory olfactory appendages. When inactivated, mutant mosquitoes displayed altered neuronal responses amines.. Beyond phenotypes, mutants display significantly impaired mating blood feeding capabilities. Our data reveal discrete pathways, suggesting as targets for design control strategies.

Language: Английский

The number of neurons in Drosophila and mosquito brains DOI Creative Commons
Joshua I. Raji, Christopher J. Potter

PLoS ONE, Journal Year: 2021, Volume and Issue: 16(5), P. e0250381 - e0250381

Published: May 14, 2021

Various insect species serve as valuable model systems for investigating the cellular and molecular mechanisms by which a brain controls sophisticated behaviors. In particular, nervous system of Drosophila melanogaster has been extensively studied, yet experiments aimed at determining number neurons in are surprisingly lacking. Using isotropic fractionator coupled with immunohistochemistry, we counted total neuronal non-neuronal cells whole brain, central optic lobe . For comparison, also populations three divergent mosquito species: Aedes aegypti , Anopheles coluzzii Culex quinquefasciatus The average adult was determined to be 199,380 ±3,400 D 217,910 ±6,180 Ae 223,020 ± 4,650 An 225,911±7,220 C mean cell count vs. lobes (101,140 ±3,650 107,270 2,720), (109,140 3,550 112,000 4,280), (105,130 3,670 107,140 3,090), (108,530 ±7,990 110,670 3,950) estimated. Each comprised 89% 2% out its population. Isotropic fractionation analyses did not identify obvious sexual dimorphism population these insects. Our study provides experimental evidence brains.

Language: Английский

Citations

100
Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block Mosquito Biting DOI Creative Commons
Laura B. Duvall, Lavoisier Ramos‐Espiritu, Kyrollos E. Barsoum

et al.

Cell, Journal Year: 2019, Volume and Issue: 176(4), P. 687 - 701.e5

Published: Feb. 1, 2019

Female Aedes aegypti mosquitoes bite humans to obtain blood develop their eggs. Remarkably, strong attraction is suppressed for days after the meal by an unknown mechanism. We investigated a role neuropeptide Y (NPY)-related signaling in long-term behavioral suppression and discovered that drugs targeting human NPY receptors modulate mosquito host-seeking. In screen of all 49 predicted Ae. peptide receptors, we identified NPY-like receptor 7 (NPYLR7) as sole target these drugs. To small-molecule agonists selective NPYLR7, performed high-throughput cell-based assay 265,211 compounds isolated six highly NPYLR7 inhibit humans. CRISPR-Cas9 null mutants are defective resistant Finally, show can biting blood-feeding on live host, suggesting novel approach control infectious disease transmission controlling behavior.Video AbstracteyJraWQiOiI4ZjUxYWNhY2IzYjhiNjNlNzFlYmIzYWFmYTU5NmZmYyIsImFsZyI6IlJTMjU2In0.eyJzdWIiOiJjZGQ0ODkyMTY5YWZhZDcyOWFjMGM2NzZkNTQyZGE1YyIsImtpZCI6IjhmNTFhY2FjYjNiOGI2M2U3MWViYjNhYWZhNTk2ZmZjIiwiZXhwIjoxNjc4NTU0NzMyfQ.IWqIlOv-k_s5VeH8vGctMuo6d2wnVQraplkJ0BqNeSWpc9I1CuK41HdQ7D0Y6zjDHGcYmCbA6N0lcR7GhcwcYlJktBF9CHD5koGyOMgn6PpFoexqUY5L9_rheIMShGWZjfq-W2eu99JcPhHcNOXLQ3nI54um-tcAKtPkF34pXJyOtVpX82kWquECPon_feVDg7YME6Znl4oyzekjKhsJzRBOO9-7XghUE78QjO2glylQt-WovwhdjdTuq8lpd2U5XEezFwsr68oTZaENO_q4uDjfzWNPOlJwE84dhLxMJYbGEIoehrmIgjzRsefINHApB-S0Y68C00Y3CXXoaHvtUw(mp4, (16.2 MB) Download video

Language: Английский

Citations

99
Sensory Discrimination of Blood and Floral Nectar by Aedes aegypti Mosquitoes DOI Creative Commons
Veronica Jové, Zhongyan Gong, Felix J.H. Hol

et al.

Neuron, Journal Year: 2020, Volume and Issue: 108(6), P. 1163 - 1180.e12

Published: Oct. 12, 2020

Language: Английский

Citations

79
Human attractive cues and mosquito host-seeking behavior DOI Creative Commons
Iliano V. Coutinho-Abreu, Jeffrey A. Riffell, Omar S. Akbari

et al.

Trends in Parasitology, Journal Year: 2021, Volume and Issue: 38(3), P. 246 - 264

Published: Oct. 19, 2021

Language: Английский

Citations

59
Spatial transcriptomic reconstruction of the mouse olfactory glomerular map suggests principles of odor processing DOI
I‐Hao Wang, Evan Murray,

Greg Andrews

et al.

Nature Neuroscience, Journal Year: 2022, Volume and Issue: 25(4), P. 484 - 492

Published: March 21, 2022

Language: Английский

Citations

50
Sparse and stereotyped encoding implicates a core glomerulus for ant alarm behavior DOI Creative Commons
Taylor Hart,

Dominic D. Frank,

Lindsey E. Lopes

et al.

Cell, Journal Year: 2023, Volume and Issue: 186(14), P. 3079 - 3094.e17

Published: June 15, 2023

Ants communicate via large arrays of pheromones and possess expanded, highly complex olfactory systems, with antennal lobes in the brain comprising up to ∼500 glomeruli. This expansion implies that odors could activate hundreds glomeruli, which would pose challenges for higher-order processing. To study this problem, we generated transgenic ants expressing genetically encoded calcium indicator GCaMP sensory neurons. Using two-photon imaging, mapped complete glomerular responses four ant alarm pheromones. Alarm robustly activated ≤6 activity maps three inducing panic our species converged on a single glomerulus. These results demonstrate that, rather than using broadly tuned combinatorial encoding, employ precise, narrowly tuned, stereotyped representations The identification central hub glomerulus behavior suggests simple neural architecture is sufficient translate pheromone perception into behavioral outputs.

Language: Английский

Citations

24
Genetic analysis of mosquito detection of humans DOI Creative Commons
Joshua I. Raji, Matthew DeGennaro

Current Opinion in Insect Science, Journal Year: 2017, Volume and Issue: 20, P. 34 - 38

Published: March 14, 2017

Mosquitoes detect the presence of humans by integrating chemosensory, thermal, and visual cues. Among these, odors are crucial for mosquito host detection. Insects have evolved a diverse repertoire receptors to their plant animal hosts. Genetic analysis these in Drosophila has set stage similar studies mosquitoes. The diversity cues involved host-seeking made designing behavioral control strategies challenge. sensory that most important detection can now be determined using genome editing. Here, we will review our current understanding salient attract mosquitoes, receptors, suggest ways forward novel olfaction-based vector strategies.

Language: Английский

Citations

86
General Visual and Contingent Thermal Cues Interact to Elicit Attraction in Female Aedes aegypti Mosquitoes DOI Creative Commons
Molly Z. Liu, Leslie B. Vosshall

Current Biology, Journal Year: 2019, Volume and Issue: 29(13), P. 2250 - 2257.e4

Published: June 27, 2019

Language: Английский

Citations

75
The quantum basis of spatiotemporality in perception and consciousness DOI
Abir U. Igamberdiev, Nikita E. Shklovskiy-Kordi

Progress in Biophysics and Molecular Biology, Journal Year: 2017, Volume and Issue: 130, P. 15 - 25

Published: Feb. 21, 2017

Language: Английский

Citations

73
Olfaction, experience and neural mechanisms underlying mosquito host preference DOI Open Access
Gabriella H. Wolff, Jeffrey A. Riffell

Journal of Experimental Biology, Journal Year: 2018, Volume and Issue: 221(4)

Published: Feb. 15, 2018

ABSTRACT Mosquitoes are best known for their proclivity towards biting humans and transmitting bloodborne pathogens, but there over 3500 species, including both blood-feeding non-blood-feeding taxa. The diversity of host preference in mosquitoes is exemplified by the feeding habits genus Malaya that feed on ant regurgitation or those from Uranotaenia favor amphibian hosts. Host also no means static, characterized behavioral plasticity allows to switch hosts when preferred unavailable learning cues associated with positive negative experiences. Here we review diverse range host-preference behaviors across family Culicidae, which includes all mosquitoes, how adaptations neural circuitry might affect changes within life history a mosquito evolutionary time-scales.

Language: Английский

Citations

72