Journal of The Royal Society Interface,
Год журнала:
2020,
Номер
17(168), С. 20200360 - 20200360
Опубликована: Июль 1, 2020
Many
models
of
gene
expression
do
not
explicitly
incorporate
a
cell
cycle
description.
Here,
we
derive
theory
describing
how
messenger
RNA
(mRNA)
fluctuations
for
constitutive
and
bursty
are
influenced
by
stochasticity
in
the
duration
timing
DNA
replication.
Analytical
expressions
moments
show
that
omitting
introduces
an
error
predicted
mean
number
mRNAs
is
monotonically
decreasing
function
η
,
which
proportional
to
ratio
mRNA
lifetime.
By
contrast,
variance
distribution
highest
intermediate
values
consistent
with
genome-wide
measurements
many
organisms.
Using
eukaryotic
data,
estimate
errors
be
at
most
3%
25%,
respectively.
Furthermore,
accurate
negative
binomial
mixture
approximation
distribution.
This
indicates
can
introduce
numbers
similar
effect
transcription.
Finally,
real
experimental
disregarding
inference
transcription
rates
larger
than
10%.
Nature Communications,
Год журнала:
2021,
Номер
12(1)
Опубликована: Март 5, 2021
Abstract
Accurate
measurements
of
promoter
activities
are
crucial
for
predictably
building
genetic
systems.
Here
we
report
a
method
to
simultaneously
count
plasmid
DNA,
RNA
transcripts,
and
protein
expression
in
single
living
bacteria.
From
these
data,
the
activity
units
RNAP/s
can
be
inferred.
This
work
facilitates
reporting
promoters
absolute
units,
variability
their
across
population,
quantitative
toll
on
cellular
resources,
all
which
provide
critical
insights
engineering.
Journal of The Royal Society Interface,
Год журнала:
2020,
Номер
17(168), С. 20200360 - 20200360
Опубликована: Июль 1, 2020
Many
models
of
gene
expression
do
not
explicitly
incorporate
a
cell
cycle
description.
Here,
we
derive
theory
describing
how
messenger
RNA
(mRNA)
fluctuations
for
constitutive
and
bursty
are
influenced
by
stochasticity
in
the
duration
timing
DNA
replication.
Analytical
expressions
moments
show
that
omitting
introduces
an
error
predicted
mean
number
mRNAs
is
monotonically
decreasing
function
η
,
which
proportional
to
ratio
mRNA
lifetime.
By
contrast,
variance
distribution
highest
intermediate
values
consistent
with
genome-wide
measurements
many
organisms.
Using
eukaryotic
data,
estimate
errors
be
at
most
3%
25%,
respectively.
Furthermore,
accurate
negative
binomial
mixture
approximation
distribution.
This
indicates
can
introduce
numbers
similar
effect
transcription.
Finally,
real
experimental
disregarding
inference
transcription
rates
larger
than
10%.
