Sequence-Independent Self-Assembly of Germ Granule mRNAs into Homotypic Clusters

Molecular Cell, Journal Year: 2020, Volume and Issue: 78(5), P. 941 - 950.e12

Published: May 27, 2020

Language: Английский

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Biomolecular condensates amplify mRNA decapping by biasing enzyme conformation

Nature Chemical Biology, Journal Year: 2021, Volume and Issue: 17(5), P. 615 - 623

Published: March 25, 2021

Language: Английский

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Single-cell measurement of plasmid copy number and promoter activity

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: March 5, 2021

Abstract Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity units RNAP/s can be inferred. This work facilitates reporting promoters absolute units, variability their across population, quantitative toll on cellular resources, all which provide critical insights engineering.

Language: Английский

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Effects of cell cycle variability on lineage and population measurements of messenger RNA abundance

Journal of The Royal Society Interface, Journal Year: 2020, Volume and Issue: 17(168), P. 20200360 - 20200360

Published: July 1, 2020

Many models of gene expression do not explicitly incorporate a cell cycle description. Here, we derive theory describing how messenger RNA (mRNA) fluctuations for constitutive and bursty are influenced by stochasticity in the duration timing DNA replication. Analytical expressions moments show that omitting introduces an error predicted mean number mRNAs is monotonically decreasing function η , which proportional to ratio mRNA lifetime. By contrast, variance distribution highest intermediate values consistent with genome-wide measurements many organisms. Using eukaryotic data, estimate errors be at most 3% 25%, respectively. Furthermore, accurate negative binomial mixture approximation distribution. This indicates can introduce numbers similar effect transcription. Finally, real experimental disregarding inference transcription rates larger than 10%.

Language: Английский

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Lanthanide-Based Optical Probes of Biological Systems

Cell chemical biology, Journal Year: 2020, Volume and Issue: 27(8), P. 921 - 936

Published: July 30, 2020

Language: Английский

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