Technologies for imaging neural activity in large volumes

Nature Neuroscience, Год журнала: 2016, Номер 19(9), С. 1154 - 1164

Опубликована: Авг. 26, 2016

Язык: Английский

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Subcellular Imaging of Voltage and Calcium Signals Reveals Neural Processing In Vivo

Cell, Год журнала: 2016, Номер 166(1), С. 245 - 257

Опубликована: Июнь 1, 2016

Язык: Английский

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Population imaging of neural activity in awake behaving mice

Nature, Год журнала: 2019, Номер 574(7778), С. 413 - 417

Опубликована: Окт. 9, 2019

Язык: Английский

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A Photostable Silicon Rhodamine Platform for Optical Voltage Sensing

Journal of the American Chemical Society, Год журнала: 2015, Номер 137(33), С. 10767 - 10776

Опубликована: Авг. 3, 2015

This paper describes the design and synthesis of a photostable, far-red to near-infrared (NIR) platform for optical voltage sensing. We developed new, sulfonated silicon rhodamine fluorophore integrated it with phenylenevinylene molecular wire create Berkeley Red Sensor Transmembrane potential, or BeRST 1 ("burst"). is first member class NIR sensitive dyes that make use photoinduced electron transfer (PeT) trigger interrogation membrane voltage. show displays bright, membrane-localized fluorescence in living cells, high photostability, excellent sensitivity neurons. Depolarization plasma results rapid increases (24% ΔF/F per 100 mV). can be used conjunction fluorescent stains organelles, Ca(2+) indicators, voltage-sensitive proteins. In addition, red-shifted spectral profile 1, relative commonly employed optogenetic actuators like ChannelRhodopsin2 (ChR2), which require blue light, enables electrophysiology The speed, sensitivity, photostability long-wavelength profiles useful noninvasive, dissection neuronal activity.

Язык: Английский

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Benchmarking Spike Rate Inference in Population Calcium Imaging

Neuron, Год журнала: 2016, Номер 90(3), С. 471 - 482

Опубликована: Май 1, 2016

Язык: Английский

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