Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems

Nature Neuroscience, Год журнала: 2017, Номер 20(8), С. 1172 - 1179

Опубликована: Июнь 26, 2017

Язык: Английский

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Novel electrode technologies for neural recordings

Nature reviews. Neuroscience, Год журнала: 2019, Номер 20(6), С. 330 - 345

Опубликована: Март 4, 2019

Язык: Английский

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Genetic Dissection of Neural Circuits: A Decade of Progress

Neuron, Год журнала: 2018, Номер 98(2), С. 256 - 281

Опубликована: Апрель 1, 2018

Tremendous progress has been made since Neuron published our Primer on genetic dissection of neural circuits 10 years ago. Since then, cell-type-specific anatomical, neurophysiological, and perturbation studies have carried out in a multitude invertebrate vertebrate organisms, linking neurons to behavioral functions. New methods allow systematic classification cell types provide access diverse neuronal for connectivity coding during behavior. Here we evaluate key advances over the past decade discuss future directions.

Язык: Английский

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Bright and photostable chemigenetic indicators for extended in vivo voltage imaging

Science, Год журнала: 2019, Номер 365(6454), С. 699 - 704

Опубликована: Авг. 1, 2019

Genetically encoded voltage indicators (GEVIs) enable monitoring of neuronal activity at high spatial and temporal resolution. However, the utility existing GEVIs has been limited by brightness photostability fluorescent proteins rhodopsins. We engineered a GEVI, called Voltron, that uses bright photostable synthetic dyes instead protein-based fluorophores, thereby extending number neurons imaged simultaneously in vivo factor 10 enabling imaging for significantly longer durations relative to GEVIs. used Voltron mice, zebrafish, fruit flies. In mouse cortex, allowed single-trial recording spikes subthreshold signals from dozens over 15-minute period continuous imaging. larval enabled precise correlation spike timing with behavior.

Язык: Английский

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The rise of three-dimensional human brain cultures

Nature, Год журнала: 2018, Номер 553(7689), С. 437 - 445

Опубликована: Янв. 1, 2018

Язык: Английский

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A genetically encoded fluorescent acetylcholine indicator for in vitro and in vivo studies

Nature Biotechnology, Год журнала: 2018, Номер 36(8), С. 726 - 737

Опубликована: Июль 9, 2018

Язык: Английский

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A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters

Nature Chemical Biology, Год журнала: 2018, Номер 14(4), С. 352 - 360

Опубликована: Фев. 26, 2018

Язык: Английский

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Challenges and opportunities for large-scale electrophysiology with Neuropixels probes

Current Opinion in Neurobiology, Год журнала: 2018, Номер 50, С. 92 - 100

Опубликована: Фев. 13, 2018

Electrophysiological methods are the gold standard in neuroscience because they reveal activity of individual neurons at high temporal resolution and arbitrary brain locations. Microelectrode arrays based on complementary metal-oxide semiconductor (CMOS) technology, such as Neuropixels probes, look set to transform these methods. probes provide ∼1000 recording sites an extremely narrow shank, with on-board amplification, digitization, multiplexing. They deliver low-noise recordings from hundreds neurons, providing a step change type data available neuroscientists. Here we discuss opportunities afforded by for large-scale electrophysiology, challenges associated processing anatomical localization, avenues further improvements technology.

Язык: Английский

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Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice

Cell, Год журнала: 2019, Номер 179(7), С. 1590 - 1608.e23

Опубликована: Дек. 1, 2019

Язык: Английский

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Optogenetic Tools for Subcellular Applications in Neuroscience

Neuron, Год журнала: 2017, Номер 96(3), С. 572 - 603

Опубликована: Ноя. 1, 2017

Язык: Английский

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