Integrins as biomechanical sensors of the microenvironment

Nature Reviews Molecular Cell Biology, Год журнала: 2019, Номер 20(8), С. 457 - 473

Опубликована: Июнь 10, 2019

Язык: Английский

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Role of Extracellular Matrix in Development and Cancer Progression

International Journal of Molecular Sciences, Год журнала: 2018, Номер 19(10), С. 3028 - 3028

Опубликована: Окт. 4, 2018

The immense diversity of extracellular matrix (ECM) proteins confers distinct biochemical and biophysical properties that influence cell phenotype. ECM is highly dynamic as it constantly deposited, remodelled, degraded during development until maturity to maintain tissue homeostasis. ECM’s composition organization are spatiotemporally regulated control behaviour differentiation, but dysregulation dynamics leads the diseases such cancer. chemical cues presented by have been appreciated key drivers for both cancer progression. However, mechanical forces present due largely ignored recently recognized play critical roles in disease progression malignant behaviour. Here, we review ways which microenvironment regulation phenotype stages human

Язык: Английский

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Crosstalk between mechanotransduction and metabolism

Nature Reviews Molecular Cell Biology, Год журнала: 2020, Номер 22(1), С. 22 - 38

Опубликована: Ноя. 13, 2020

Язык: Английский

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High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

Advanced Materials, Год журнала: 2020, Номер 32(9)

Опубликована: Янв. 16, 2020

Abstract Materials patterned with high‐aspect‐ratio nanostructures have features on similar length scales to cellular components. These surfaces are an extreme topography the level and become useful tools for perturbing sensing environment. Motivation comes from ability of deliver cargoes into cells tissues, access intracellular environment, control cell behavior. structures directly perturb cells' sense respond external forces, influencing fate, enabling new mechanistic studies. Through careful design their nanoscale structure, these systems act as biological metamaterials, eliciting unusual responses. While predominantly used interface eukaryotic cells, there is growing interest in nonanimal prokaryotic interfacing. Both experimental theoretical studies attempted develop a understanding observed behaviors, focusing cell–nanostructure interface. This review considers how nanostructured both stimulate systems.

Язык: Английский

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Mechanobiology of Macrophages: How Physical Factors Coregulate Macrophage Plasticity and Phagocytosis

Annual Review of Biomedical Engineering, Год журнала: 2019, Номер 21(1), С. 267 - 297

Опубликована: Июнь 4, 2019

In addition to their early-recognized functions in host defense and the clearance of apoptotic cell debris, macrophages play vital roles tissue development, homeostasis, repair. If misregulated, they steer progression many inflammatory diseases. Much progress has been made understanding mechanisms underlying macrophage signaling, transcriptomics, proteomics, under physiological pathological conditions. Yet, detailed that tune circulating monocytes/macrophages tissue-resident polarization, differentiation, specification, functional plasticity remain elusive. We review how physical factors affect phenotype function, including hunt for particles pathogens, as well implications phagocytosis, autophagy, polarization from proinflammatory prohealing phenotype. further discuss this knowledge can be harnessed regenerative medicine design new drugs immune-modulatory drug delivery systems, biomaterials, scaffolds.

Язык: Английский

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Matrix stiffness induces a tumorigenic phenotype in mammary epithelium through changes in chromatin accessibility

Nature Biomedical Engineering, Год журнала: 2019, Номер 3(12), С. 1009 - 1019

Опубликована: Июль 8, 2019

Язык: Английский

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Surface Roughness and Substrate Stiffness Synergize To Drive Cellular Mechanoresponse

Nano Letters, Год журнала: 2019, Номер 20(1), С. 748 - 757

Опубликована: Дек. 10, 2019

Material surface topographic features have been shown to be crucial for tissue regeneration and treatment of implanted devices. Many biomaterials were investigated with respect the response cells on roughness. However, some conclusions even conflicted each other due unclear interplay substrate elastic as well lack mechanistic studies. Herein, wide-scale roughness gradient hydrogels, integrating from nanoscale microscale controllable stiffness, developed via soft lithography precise morphology. Based this promising platform, we systematically studied mechanosensitive human mesenchymal stem (MSCs) a broad range roughnesses (200 nm 1.2 μm Rq) different stiffnesses. We observed that MSCs responded in stiffness-dependent manner by reorganizing hierarchical structure. Surprisingly, cellular mechanoresponse osteogenesis obviously enhanced very hydrogels (3.8 kPa) high roughness, which was comparable or better than smooth stiff substrates. These findings extend our understanding interactions between biomaterials, highlighting an effective noninvasive approach regulate cell fate synergetic physical cues.

Язык: Английский

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Innovative Tools for Mechanobiology: Unraveling Outside-In and Inside-Out Mechanotransduction

Frontiers in Bioengineering and Biotechnology, Год журнала: 2019, Номер 7

Опубликована: Июль 16, 2019

Cells and tissues can sense react to the modifications of physico-chemical properties extracellular environment (ECM) through integrin-based adhesion sites adapt their physiological response in a process called mechanotransduction. Due critical localization at cell-ECM interface, transmembrane integrins are mediators bidirectional signaling, playing key role « outside-in » inside-out signal transduction. After presenting basic conceptual fundamentals related field mechanobiology, we review current state-of-the-art technologies that facilitate understanding mechanotransduction signaling pathways. Finally, highlight innovative technological developments help advance our molecular mechanisms involved nuclear

Язык: Английский

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Multi-domain translation between single-cell imaging and sequencing data using autoencoders

Nature Communications, Год журнала: 2021, Номер 12(1)

Опубликована: Янв. 4, 2021

Abstract The development of single-cell methods for capturing different data modalities including imaging and sequencing has revolutionized our ability to identify heterogeneous cell states. Different provide perspectives on a population cells, their integration is critical studying cellular heterogeneity its function. While various have been proposed integrate modalities, coupling an open challenge. We here present approach integrating vastly by learning probabilistic between the using autoencoders map shared latent space. validate this RNA-seq chromatin images distinct subpopulations human naive CD4+ T-cells that are poised activation. Collectively, provides framework translate cannot yet be measured within same diverse applications in biomedical discovery.

Язык: Английский

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How Mechanical Forces Shape Plant Organs

Current Biology, Год журнала: 2021, Номер 31(3), С. R143 - R159

Опубликована: Фев. 1, 2021

Язык: Английский

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