Characteristics and sources of tissue-resident memory T cells in psoriasis relapse

Current Research in Immunology, Год журнала: 2023, Номер 4, С. 100067 - 100067

Опубликована: Янв. 1, 2023

Tissue-resident memory T cells (Trm) are a sub-population of that reside in skin tissue. Recent studies have revealed potential role Trm the reoccurrence psoriasis, as these tend to be profusely infiltrated lesions observed during psoriasis relapse. can classified into CD8+ distributed mainly epidermis and CD4+ dermis. is derived from circulating CD49a−CD8+ takes crucial In contrast, may originate exTh17 exTreg emerging inflammatory process. Since IL-23 activate Trm, neutralizing antibodies against suggested more effective clinical treatment. This review will focus on relapsed reveal their mechanisms pathogenesis, relapse transformation psoriasis.

Язык: Английский

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Interleukin‐17 alleviates erastin‐induced alveolar bone loss by suppressing ferroptosis via interaction between NRF2 and p‐STAT3

Journal Of Clinical Periodontology, Год журнала: 2023, Номер 51(2), С. 233 - 250

Опубликована: Ноя. 14, 2023

Abstract Aim To investigate the relationship between interleukin‐17 (IL‐17), ferroptosis and osteogenic differentiation. Materials Methods We first analysed changes in ferroptosis‐related molecules experimental periodontitis models. The effects of erastin, a small‐molecule inducer, IL‐17 on alveolar bone loss repair animal models were then investigated. Primary mouse mandibular osteoblasts exposed to erastin vitro. Ferroptosis‐ osteogenesis‐related genes proteins detected. Further, siRNA, immunofluorescence co‐localization immunoprecipitation used confirm roles nuclear factor erythroid‐2‐related 2 (NRF2) phosphorylated signal transducer activator transcription 3 (p‐STAT3), as well their interaction. Results levels NRF2, glutathione peroxidase 4 solute carrier family 7 member 11 lower ligated tissues than normal periodontal tissues. Alveolar an vivo model was aggravated by alleviated IL‐17. In vitro, ameliorated erastin‐inhibited differentiation reversing ferroptosis. Altered NRF2 expression correlated with osteogenesis. Furthermore, physical interaction p‐STAT3 confirmed nucleus. + erastin‐stimulated osteoblasts, p‐STAT3–NRF2 complex might actively participate downstream ferroptosis‐ genes. Conclusions administration conferred resistance erastin‐induced osteoblast possible mechanism may involve directly interacting NRF2.

Язык: Английский

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Re-establishing immune tolerance in multiple sclerosis: focusing on novel mechanisms of mesenchymal stem cell regulation of Th17/Treg balance

Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Июль 15, 2024

The T-helper 17 (Th17) cell and regulatory T (Treg) axis plays a crucial role in the development of multiple sclerosis (MS), which is regarded as an immune imbalance between pro-inflammatory cytokines maintenance tolerance. Mesenchymal stem (MSC)-mediated therapies have received increasing attention MS research. In its animal model experimental autoimmune encephalomyelitis, MSC injection was shown to alter differentiation CD4

Язык: Английский

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Mesenchymal stem cell–derived extracellular vesicles in periodontal bone repair

Journal of Molecular Medicine, Год журнала: 2025, Номер unknown

Опубликована: Янв. 16, 2025

Язык: Английский

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Extracellular vesicles: translational research and applications in neurology

Nature Reviews Neurology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 3, 2025

Язык: Английский

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Zinc finger DHHC-type palmitoyltransferase 13-mediated S-palmitoylation of GNA13 from Sertoli cell-derived extracellular vesicles inhibits autophagy in spermatogonial stem cells

Cell Communication and Signaling, Год журнала: 2025, Номер 23(1)

Опубликована: Апрель 9, 2025

Extracellular vesicles (EVs) originating from testicular somatic cells act as pivotal intermediaries in cell signaling crosstalk between spermatogenic and the microenvironment. The intricate balance palmitoylation depalmitoylation governs positioning of protein cargos on membrane, thereby influencing cellular activities by concentrating these proteins EVs for delivery to recipient cells. Here, we reveal that GNA13 undergoes specific S-palmitoylation at Cys14 Cys18 residues Sertoli (SCs), a modification essential its localization plasma membrane. We identify DHHC13, member zinc finger DHHC-type palmitoyltransferase family catalyzes S-palmitoylation, enzyme responsible this critical post-translational modification. Additionally, is indispensable selective enrichment emanating SCs. Intriguingly, discovered presence palmitoylated SC-derived significantly downregulates autophagy levels spermatogonial stem (SSCs), inhibition attenuates interaction with ARHGEF12 which leads diminished RhoA activity consequent elevation SSCs. Our results illuminate crucial role DHHC13-mediated modulating SSCs through SCs-derived EVs, suggesting PM-GNA13-EV may serve potential candidate further exploration addressing fertility-related challenges during spermatogenesis.

Язык: Английский

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Harnessing exosomes for targeted drug delivery systems to combat brain cancer

Cancer Cell International, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 15, 2025

Abstract Brain cancer remains a significant challenge in the field of oncology, primarily because its aggressive nature and limited treatment options available. Conventional therapies often fall short effectively targeting tumor cells, while sparing healthy brain tissue from collateral damage. However, exosomes are now recognized as promising nanocarriers for targeted drug delivery. These naturally occurring extracellular vesicles can cross blood–brain barrier selectively interact with cells. Utilizing delivery vehicles offers novel approach potential therapy. By encapsulating therapeutic agents within exosomes, drugs be specifically to maximizing their impact whilst minimizing damage tissue. Furthermore, modified display molecules that recognize bind further enhancing precision efficacy. While exosome-based show potential, scalability, purification, clinical application challenges remain. The scalability exosome production, modification techniques hurdle must overcome translation. Additionally, intricate interactions between microenvironment necessitate research optimize outcomes. review explores applications future perspectives advancing treatment.

Язык: Английский

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Emerging Role and Mechanism of Mesenchymal Stem Cells-Derived Extracellular Vesicles in Rheumatic Disease

Journal of Inflammation Research, Год журнала: 2024, Номер Volume 17, С. 6827 - 6846

Опубликована: Сен. 1, 2024

Mesenchymal stem cells (MSCs) are pluripotent derived from mesoderm. Through cell-to-cell contact or paracrine effects, they carry out biological tasks like immunomodulatory, anti-inflammatory, regeneration, and repair. Extracellular vesicles (EVs) the primary mechanism for regulation of MSCs. They deliver proteins, nucleic acids, lipids, other active compounds to various tissues organs, thus facilitating intercellular communication. Rheumatic diseases may be treated using MSCs MSC-derived EVs (MSC-EVs) due their immunomodulatory capabilities, according mounting data. Since MSC-EVs have low immunogenicity, high stability, similar effects as themselves, advantageous over cell therapy potential therapeutic applications in rheumatoid arthritis, systemic erythematosus lupus, sclerosis, Sjogren's syndrome, diseases. This review integrates recent advances characteristics, functions, molecular mechanisms rheumatic provides a new understanding pathogenesis MSC-EV-based treatment strategies.

Язык: Английский

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USP26 Combats Age‐Related Declines in Self‐Renewal and Multipotent Differentiation of BMSC by Maintaining Mitochondrial Homeostasis

Advanced Science, Год журнала: 2024, Номер 11(44)

Опубликована: Окт. 8, 2024

Abstract Age‐related declines in self‐renewal and multipotency of bone marrow mesenchymal stem cells (BMSCs) limit their applications tissue engineering clinical therapy. Thus, understanding the mechanisms behind BMSC senescence is crucial for maintaining rejuvenation multipotent differentiation capabilities BMSCs. This study reveals that impaired USP26 expression BMSCs leads to mitochondrial dysfunction, ultimately resulting aging age‐related Specifically, decreased results protein levels Sirtuin 2 due its ubiquitination degradation, which dysfunction multilineage potentials. Additionally, a result dampened hypoxia‐inducible factor 1α (HIF‐1α) expression. HIF‐1α facilitates transcriptional by increasing promoter activity through binding ‐191 — ‐198 bp ‐262 ‐269 regions on promoter. Therefore, identification as being correlated with BMSCs, along action mechanisms, suggests represents novel therapeutic target combating

Язык: Английский

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Mesenchymal stem cell-derived exosomes can alleviate GVHD and preserve the GVL effect in allogeneic stem cell transplantation animal models

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Дек. 6, 2023

Background Mesenchymal stem cells (MSCs) can alleviate graft-versus-host disease (GVHD) in hematopoietic cell transplantation (HSCT). MSCs-derived exosomes (MEXs) mirror the biological function of their parent cells. Whether MEXs GVHD like or not is unclear. In this study, we investigate effects on and graft-versus-leukemia (GVL) effect vitro HSCT animal models. Method MSCs were produced using bone marrow mononuclear (MNCs), separated from supernatants MSCs. Electron microscopy, western blot, nanoparticle tracking analysis (NTA) used to determine characteristics MEXs. The immunomodulatory GVL examined vivo . Result Like other cell-type derived exosomes, our data revealed that also disc-shaped vesicles with a diameter 100–200 nm under electron microscopy positive for exosomal hallmark proteins. notably inhibit expression costimulatory molecules functional cytokine secretion dendritic (DCs). Meanwhile, exert suppressive T lymphocyte proliferation activation. Moreover, encourage polarization macrophages toward M2 type. models, promote differentiation Treg spleens, decrease score, increase survival rate mice, preserve cytotoxic antileukemia CD8 + lymphocytes recipient mice. Conclusion These findings showed by inhibiting DCs, macrophages, lymphocytes. model, ameliorate clinical symptoms GVHD, while maintaining antitumor Therefore, it be inferred separate HSCT. Our study suggests have broad application potential prevention treatment near future.

Язык: Английский

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