eGastroenterology,
Год журнала:
2024,
Номер
2(4), С. e100129 - e100129
Опубликована: Дек. 1, 2024
The
intestinal
epithelium
serves
as
an
essential
interface
between
the
host
and
microbiota,
regulating
innate
adaptive
immunity,
absorption
of
nutrients
systemic
metabolism,
mediating
bidirectional
communication
with
nervous
system.
suffers
constant
challenges
to
proteostasis
machinery
due
its
exposure
dynamically
changing
microbial
laden
lumenal
gut
environment
high
secretory
demand
placed
on
multiple
epithelial
cell
types
accommodate
physiology-especially
goblet,
enteroendocrine
Paneth
cells.
In
all
cases,
cells
require
active
unfolded
protein
response
(UPR)
sustain
their
physiological
function,
main
pathway
that
monitors
adjusts
function
changes
in
environment.
A
specialised
endoplasmic
reticulum
(ER)
stress
sensor
uniquely
expressed
lining
mucosal
surfaces,
termed
inositol-requiring
transmembrane
kinase/endoribonuclease
β,
has
specific
roles
homeostasis,
mucus
production
microbiota.
Chronic
ER
or
genetic
mutations
affecting
key
UPR
mediators
contribute
occurrence
inflammatory
bowel
disease
ulcerative
colitis,
addition
colon
cancer.
Here,
we
review
recent
advances
linking
physiology
disease.
Therapeutic
strategies
alleviate
enforce
improve
ageing
diseases
are
also
discussed.
Experimental and Molecular Pathology,
Год журнала:
2024,
Номер
139, С. 104923 - 104923
Опубликована: Авг. 17, 2024
Older
people
experience
a
greater
incidence
of
lower
bowel
disorders,
including
constipation.
Causes
can
include
factors
associated
with
growing
older,
such
as
use
medications
or
disease,
but
compounded
by
degenerative
changes
within
the
wall.
It
has
been
suggested
that
latter
is
exacerbated
loss
an
effective
mucosal
barrier
to
luminal
contents.
In
human
colon,
little
known
about
impact
ageing
on
key
components
this
barrier,
namely
goblet
cells
and
mucin
content.
Biology,
Год журнала:
2025,
Номер
14(2), С. 110 - 110
Опубликована: Янв. 22, 2025
The
intestinal
area
is
composed
of
diverse
cell
types
that
harmonize
gut
homeostasis,
which
influenced
by
both
endogenous
and
exogenous
factors.
Notably,
the
environment
intestine
exposed
to
several
mechanical
forces,
including
shear
stress
generated
fluid
flow,
compression
stretch
luminal
contents
peristaltic
waves
intestine,
stiffness
attributed
extracellular
matrix.
These
forces
play
critical
roles
in
regulation
proliferation,
differentiation,
migration.
Many
efforts
have
been
made
simulate
actual
vitro.
three-dimensional
organoid
culture
system
has
emerged
as
a
powerful
tool
for
studying
mechanism
epithelial
barrier,
mimicking
rapidly
renewing
epithelium
from
stem
cells
(ISCs)
vivo.
However,
many
aspects
how
such
stress,
stiffness,
compression,
influence
remain
unresolved.
Here,
we
review
recent
studies
elucidating
impact
on
immunity,
interaction
with
microbiome,
diseases.
Journal of Inflammation Research,
Год журнала:
2025,
Номер
Volume 18, С. 1839 - 1853
Опубликована: Фев. 1, 2025
Purpose:
An
increasing
proportion
of
the
aging
population
has
led
to
a
rapid
increase
in
number
elderly
patients
with
ulcerative
colitis
(UC).
However,
molecular
mechanisms
by
which
causes
UC
remain
unclear.
In
this
study,
we
explored
role
aging-related
genes
(ARGs)
pathogenesis
and
diagnosis
prediction.
Methods:
Gene
expression
data
were
obtained
from
four
independent
datasets
(GSE75214,
GSE87466,
GSE94648,
GSE169568)
GEO
database,
ARGs
derived
multiple
public
databases.
After
identifying
UC-related
ARGs,
consistent
clustering
was
performed
screen
subtypes,
followed
exploration
differences
immune
microenvironment
pathways
between
distinct
subtypes.
Next,
core
module
screened
using
WGCNA
then
hub
characterized
LASSO
random
forest
methods.
Besides,
associations
genes,
cells,
key
explored.
Finally,
levels
determined
dextran
sulfate
sodium
(DSS)-induced
mouse
model
qRT-PCR.
Results:
samples
classified
into
two
subtypes
(1
2),
displayed
significant
landscape
JAK/STAT
signaling
pathways.
A
series
machine
learning
algorithms
used
feature
(ETS1
IL7R)
establish
diagnostic
model,
exhibited
satisfactory
efficiency.
addition,
these
closely
associated
infiltration
specific
cells
(such
as
neutrophils,
memory
B
M2
macrophages)
well
pathway.
Later,
experimental
validation
confirmed
that
ETS1
markedly
increased
UC.
Conclusion:
Overall,
aging,
dysregulation,
process
are
associated.
The
identified
particularly
ETS1,
could
serve
novel
biomarkers
for
These
findings
have
potential
enhance
understanding
age-related
Keywords:
colitis,
learning,
microenvironment,
biomarker
Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 17, 2025
Abstract
In
fetal
patients
with
intestinal
atresia,
surgical
resection
often
leads
to
short
bowel
syndrome,
necessitating
organ
transplantation.
Owing
a
shortage
of
donors,
alternatives
such
as
3D‐bioprinted
artificial
intestines
are
receiving
increased
interest.
However,
the
fabrication
transplantable
integrating
tri‐cultures
viable
functional
cells
remains
challenging.
This
study
introduces
an
innovative
method
for
fabricating
tri‐cultured
tubular
mesh
intestine
(TTMI)
myofibroblasts,
endothelial,
and
epithelial
cells.
Low‐concentration
gelatin
methacryloyl
(GelMA)
bioink
is
employed
improve
cell
viability,
dual
cooling
module
incorporated
cool
both
GelMA
printing
area
printability.
To
mechanical
properties
TTMI
transplant
stability,
multi‐head
four‐axis
bioprinter
used
apply
bioinks
polycaprolactone
(PCL).
The
final
four‐layered
comprises
three
PCL;
two
middle
layers
printed
enable
cell‐to‐cell
interactions.
technology
can
be
fabricate
well
other
organs
consisting
different
cells,
ultimately
enhancing
availability
tissues
transplantation
therapy.
Molecular Nutrition & Food Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 24, 2025
1,3-Dioleoyl-2-palmitoyl-glycerol
(OPO)
is
a
specific
triglyceride
in
human
breast
milk,
and
it
has
been
added
to
infant
formula
mimic
milk
fat.
Existing
studies
only
focused
on
its
effects
fatty
acid
calcium
absorption,
as
well
the
intestinal
microbial
composition;
however,
of
OPO
early-life
development
intestine
were
still
unclear.
Our
study
explored
epithelial
structure
barrier
construction
neonatal
mice
involvement
microorganisms.
supplementation
significantly
increased
number
stem
cells,
which
turn
promoted
villus
crypt,
goblet
cell
Paneth
differentiation.
also
promotes
integrity
by
increasing
expression
mucin
2,
lysozyme
1,
tight
junction
proteins.
Furthermore,
benefits
associated
with
higher
abundance
beneficial
bacteria
(unclassified_f_Muribaculaceae,
Akkermansia,
Bifidobacterium,
Blautia)
elevated
butyrate
levels.
This
demonstrates
efficacy
health
beyond
defecation,
expands
understanding
biological
functions
OPO,
application
products
targeting
special
populations,
such
elderly
or
individuals
fragility
injury.
