Simvastatin/hydrogel-loaded 3D-printed titanium alloy scaffolds suppress osteosarcoma via TF/NOX2-associated ferroptosis while repairing bone defects DOI Creative Commons
Zehao Jing, Wanqiong Yuan, Jiedong Wang

и другие.

Bioactive Materials, Год журнала: 2023, Номер 33, С. 223 - 241

Опубликована: Ноя. 17, 2023

Postoperative anatomical reconstruction and prevention of local recurrence after tumor resection are two vital clinical challenges in osteosarcoma treatment. A three-dimensional (3D)-printed porous Ti6Al4V scaffold (3DTi) is an ideal material for reconstructing critical bone defects with numerous advantages over traditional implants, including a lower elasticity modulus, stronger bone-implant interlock, larger drug-loading space. Simvastatin multitarget drug anti-tumor osteogenic potential; however, its efficiency unsatisfactory when delivered systematically. Here, simvastatin was loaded into 3DTi using thermosensitive poly (lactic-co-glycolic) acid (PLGA)-polyethylene glycol (PEG)-PLGA hydrogel as carrier to exert anti-osteosarcoma effects. Newly constructed simvastatin/hydrogel-loaded (Sim-3DTi) comprehensively appraised, newfound mechanism explained. Specifically, defect model rabbit condyles, Sim-3DTi exhibited enhanced osteogenesis, in-growth, osseointegration compared alone, greater morphogenetic protein 2 expression. In our nude mice model, loading reduced volume by 59%-77 % without organic damage, implying good activity biosafety. Furthermore, induced ferroptosis upregulating transferrin nicotinamide adenine dinucleotide phosphate oxidase levels both

Язык: Английский

Titanium alloy composited with dual-cytokine releasing polysaccharide hydrogel to enhance osseointegration via osteogenic and macrophage polarization signaling pathways DOI Creative Commons
Ya Ping Wang, Zujian Feng, Xiang Liu

и другие.

Regenerative Biomaterials, Год журнала: 2022, Номер 9

Опубликована: Янв. 1, 2022

Titanium alloy has been widely used in orthopedic surgeries as bone defect filling. However, the regeneration of high-quality new bones is limited due to pro-inflammatory microenvironment around implants, resulting a high occurrence rate implant loosening or failure osteological therapy. In this study, extracellular matrix-mimetic polysaccharide hydrogel co-delivering BMP-2 and interleukin (IL)-4 was composited with 3D printed titanium promote osseointegration regulate macrophage response create pro-healing defect. Notably, it discovered from bioinformatics data that IL-4 could affect each other through multiple signal pathways achieve synergistic effect toward osteogenesis. The composite scaffold significantly promoted osteoblast differentiation proliferation human marrow mesenchyme stem cells (hBMSCs). repair large-scale femur rat indicated dual-cytokine-delivered manipulate lower inflammatory level situ by polarizing macrophages M2 phenotype, superior efficacy mature over treatment native an individual cytokine. Collectively, work highlights importance M2-type macrophages-enriched immune-environment healing. biomimetic hydrogel-metal versatile advanced for accelerating vivo regeneration, holding great promise treating diseases.

Язык: Английский

Процитировано

35
MiR‐26a‐tetrahedral framework nucleic acids mediated osteogenesis of adipose‐derived mesenchymal stem cells DOI Creative Commons

Xiaoru Shao,

Zhong Hu,

Yuxi Zhan

и другие.

Cell Proliferation, Год журнала: 2022, Номер 55(7)

Опубликована: Июнь 5, 2022

Delivery systems that provide time and space control have a good application prospect in tissue regeneration applications, as they can effectively improve the process of wound healing repair. In our experiments, we constructed novel micro-RNA delivery system by linking framework nucleic acid nanomaterials to micro-RNAs promote osteogenic differentiation mesenchymal stem cells.To verify successful preparation tFNAs-miR-26a, size tFNAs-miR-26a were observed non-denaturing polyacrylamide gel electrophoresis dynamic light scattering techniques. The expression differentiation-related genes proteins was investigated confocal microscope, PCR western blot detect impact on ADSCs. And finally, Wnt/β-catenin signaling pathway related detected study relevant mechanism.By adding this complex, ability cells significantly improved, alkaline phosphatase (ALP) surface cell membrane formation calcium nodules increased days 7 14 induction differentiation, respectively. Gene protein levels ALP (an early marker associated with differentiation), RUNX2 (a metaphase marker), OPN late marker) increased. We also studied mechanism action found complex promoted activating canonical Wnt pathway.This may new research direction for bone regeneration.

Язык: Английский

Процитировано

33
Chitosan based photothermal scaffold fighting against bone tumor-related complications: Recurrence, infection, and defects DOI
Yao Zhao, Peng Xu, Xiaoyang Xu

и другие.

Carbohydrate Polymers, Год журнала: 2022, Номер 300, С. 120264 - 120264

Опубликована: Окт. 26, 2022

Язык: Английский

Процитировано

33
Self-Assembly-Driven Bi2S3Nanobelts Integrated a Silk-Fibroin-Based 3D-Printed Aerogel-Based Scaffold with a Dual-Network Structure for Photothermal Bone Cancer Therapy DOI

Noor Al-Jawuschi,

Shiyi Chen,

Nahal Abie

и другие.

Langmuir, Год журнала: 2023, Номер 39(12), С. 4326 - 4337

Опубликована: Март 17, 2023

Multifunctional all-in-one biomaterial combining the therapeutic and regeneration functionalities for successive tumor therapy tissue is in high demand interdisciplinary research. In this study, a three-dimensional (3D) aerogel-based composite scaffold with dual-network structure generated through self-assembly photo-cross-linking combined properties of photothermally triggered controlled anticancer drug release photothermal cancer cell ablation was successfully fabricated. The fabrication composites consists silk fibroin methacrylate (SF–MA) biopolymer incorporated hydrothermally driven bismuth sulfide (Bi2S3) nanobelts, followed by photo-cross-linking-assisted 3D-printing process. developed scaffolds presented hierarchically organized porosity excellent conversion thanks to strong near-infrared (NIR) photon absorption Bi2S3 nanobelts inside matrix. heat mediated laser irradiation has not only prolonged but also significantly ablated bone cells adhered on scaffold. addition, 3D have demonstrated biodegradability organic inorganic network constituents at different media, enabling them as potential implants be replaced de novo tissue. combination chemotherapy therapy, multifunctional 3D-printed aerogel expected an implantable material engineering (BTE) regeneration.

Язык: Английский

Процитировано

17
Simvastatin/hydrogel-loaded 3D-printed titanium alloy scaffolds suppress osteosarcoma via TF/NOX2-associated ferroptosis while repairing bone defects DOI Creative Commons
Zehao Jing, Wanqiong Yuan, Jiedong Wang

и другие.

Bioactive Materials, Год журнала: 2023, Номер 33, С. 223 - 241

Опубликована: Ноя. 17, 2023

Postoperative anatomical reconstruction and prevention of local recurrence after tumor resection are two vital clinical challenges in osteosarcoma treatment. A three-dimensional (3D)-printed porous Ti6Al4V scaffold (3DTi) is an ideal material for reconstructing critical bone defects with numerous advantages over traditional implants, including a lower elasticity modulus, stronger bone-implant interlock, larger drug-loading space. Simvastatin multitarget drug anti-tumor osteogenic potential; however, its efficiency unsatisfactory when delivered systematically. Here, simvastatin was loaded into 3DTi using thermosensitive poly (lactic-co-glycolic) acid (PLGA)-polyethylene glycol (PEG)-PLGA hydrogel as carrier to exert anti-osteosarcoma effects. Newly constructed simvastatin/hydrogel-loaded (Sim-3DTi) comprehensively appraised, newfound mechanism explained. Specifically, defect model rabbit condyles, Sim-3DTi exhibited enhanced osteogenesis, in-growth, osseointegration compared alone, greater morphogenetic protein 2 expression. In our nude mice model, loading reduced volume by 59%-77 % without organic damage, implying good activity biosafety. Furthermore, induced ferroptosis upregulating transferrin nicotinamide adenine dinucleotide phosphate oxidase levels both

Язык: Английский

Процитировано

17