bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Май 14, 2025
Abstract
Background
Social
behaviour
encompasses
the
wide
range
of
interactions
that
occur
between
members
same
species.
In
humans,
disruptions
in
social
are
characteristic
many
neuropsychiatric
disorders,
where
both
genetic
risk
factors
and
synaptic
dysfunctions
can
contribute
to
phenotype.
Among
genes
implicated
regulation,
adhesion
protein
leucine-rich
repeat
transmembrane
4
(LRRTM4)
has
been
identified
as
a
key
player
maintaining
function
neuronal
circuit
integrity.
Despite
its
established
role
nervous
system,
potential
involvement
LRRTM4
modulating
contribution
deficits
yet
be
explored.
Methods
current
study,
we
used
zebrafish
study
how
deletion
lrrtm4l1
,
orthologue
affects
sociality.
For
this,
homozygous
knockout
(KO)
was
analysed
multiple
behavioural
assays
brain
transcriptome
mutant
animals
investigated
by
RNAseq.
Results
KO
displayed
pro-social
phenotype
assays.
Groups
formed
more
cohesive
shoals
individuals
spent
time
vicinity
conspecifics
during
interaction
test.
They
were
also
less
aggressive
contrast
wild-type
did
not
differentiate
their
with
known
unknown
groups
fish.
Neurotranscriptomic
analysis
revealed
560
differentially
expressed
including
changes
glutamatergic
neurotransmitter
signalling,
tryptophan-
kynurenine
metabolism
plasticity.
Conclusion
These
findings
suggest
is
an
important
regulator
zebrafish.
translational
perspective,
promising
therapeutic
target
warrants
further
investigation
framework
conditions
characterized
major
impairments.
Abstract
Autism
spectrum
disorder
(ASD)
is
a
neurodevelopmental
condition
marked
by
social
and
behavioral
impairments,
emerging
in
early
childhood
with
unclear
causes.
The
primary
aim
of
this
study
to
investigate
shifts
the
functional
gradients
underlying
hierarchical
brain
network
organization
ASD
assess
their
potential
contribution
clinical
symptom
severity.
Resting-state
magnetic
resonance
imaging
was
used
examine
changes
across
seven
major
networks
cohort
52
individuals
40
healthy
controls.
In
somatomotor
network,
neither
first
nor
third
gradient
showed
significant
group
differences;
however,
two
regions—right
paracentral
lobule
right
postcentral
gyrus—exhibited
differences
second
gradient.
frontoparietal
only
left
middle
frontal
gyrus
difference.
For
ventral
attention
exhibited
insula,
median
cingulate
paracingulate
gyri.
default
mode
all
three
statistically
differences.
These
results
suggest
neuroimaging
biomarkers
for
assessing
severity
preschool-aged
children.
Frontiers in Neural Circuits,
Год журнала:
2025,
Номер
19
Опубликована: Май 14, 2025
Autism
spectrum
disorder
(ASD)
is
associated
with
disruptions
in
social
behavior
and
the
neural
circuitry
behind
it.
Very
little
data
available
on
mechanisms
that
are
responsible
for
lack
of
motivation
to
reunite
conspecifics
during
isolation.
It
as
important
investigate
changes
reduce
end
isolation,
those
underlying
reactions
stimuli.
Using
a
rodent
model
prenatal
valproic
acid
(VPA)
exposure,
we
investigated
how
isolation
affects
activation
key
brain
nuclei
involved
processing
stress
regulation.
Juvenile
male
C57BL/6
mice
were
treated
prenatally
VPA
or
saline
(CTR)
subjected
24
h
from
their
cage
mates,
activity
assessed
via
c-Fos
immunohistochemistry.
Based
correlational
activations
reconstructed
analyzed
functional
connectome
observed
regions.
Control
animals
exhibited
elevated
expression
regions
central
mesolimbic
reward
system
(MRS),
network
(SBN),
stress-related
networks,
interpeduncular
nucleus
(IPN)
at
core,
compared
VPA-treated
animals.
Functional
analysis
revealed
more
widespread
but
less
specific
pattern
connectivity
These
findings
suggest
exposure
disrupts
certain
circuits
related
regulation,
offering
an
insight
into
altered
perception
ASD
models,
highlighting
potential
therapeutic
targets.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Май 14, 2025
Abstract
Background
Social
behaviour
encompasses
the
wide
range
of
interactions
that
occur
between
members
same
species.
In
humans,
disruptions
in
social
are
characteristic
many
neuropsychiatric
disorders,
where
both
genetic
risk
factors
and
synaptic
dysfunctions
can
contribute
to
phenotype.
Among
genes
implicated
regulation,
adhesion
protein
leucine-rich
repeat
transmembrane
4
(LRRTM4)
has
been
identified
as
a
key
player
maintaining
function
neuronal
circuit
integrity.
Despite
its
established
role
nervous
system,
potential
involvement
LRRTM4
modulating
contribution
deficits
yet
be
explored.
Methods
current
study,
we
used
zebrafish
study
how
deletion
lrrtm4l1
,
orthologue
affects
sociality.
For
this,
homozygous
knockout
(KO)
was
analysed
multiple
behavioural
assays
brain
transcriptome
mutant
animals
investigated
by
RNAseq.
Results
KO
displayed
pro-social
phenotype
assays.
Groups
formed
more
cohesive
shoals
individuals
spent
time
vicinity
conspecifics
during
interaction
test.
They
were
also
less
aggressive
contrast
wild-type
did
not
differentiate
their
with
known
unknown
groups
fish.
Neurotranscriptomic
analysis
revealed
560
differentially
expressed
including
changes
glutamatergic
neurotransmitter
signalling,
tryptophan-
kynurenine
metabolism
plasticity.
Conclusion
These
findings
suggest
is
an
important
regulator
zebrafish.
translational
perspective,
promising
therapeutic
target
warrants
further
investigation
framework
conditions
characterized
major
impairments.
