Green and ecofriendly biosynthesis of selenium nanoparticles using Urtica dioica (stinging nettle) leaf extract: Antimicrobial and anticancer activity

Biotechnology Journal, Год журнала: 2021, Номер 17(2)

Опубликована: Ноя. 8, 2021

Plant extract is affordable and does not require any particular conditions; rapid production of nanoparticles using plants offers more advantages than other approaches. Selenium (SeNPs) have received much attention in the last decade due to SeNPs diverse different applications. Herein, this study aimed biosynthesize aqueous Urtica dioica leaf through green ecofriendly method. Moreover fully characterize techniques, evaluate it for antimicrobial activity as well anticancer activity.SeNPs were biosynthesis U. (stinging nettle). The biosynthesized characterized UV-visible spectroscopy (UV-Vis), X-ray diffraction (XRD), Fourier-transform infrared (FTIR), scanning electron microscopy (SEM), energy dispersive (EDX), transmission (TEM), thermal-gravimetric analysis (TGA). Antimicrobial activities assessed. Results illustrated that exhibited promising antibacterial against Gram-positive Gram-negative bacteria, unicellular multi-cellular fungi. Moreover, minimal-inhibitory concentration (MIC) Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus 250, 31.25, 500 μg mL-1 , respectively, while 62.5, 15.62, 7.81 Candida albicans, Aspergillus fumigatus, niger, flavus, respectively. cytotoxicity was performed on Vero normal-cell line CCL-81, where IC50 173.2 .For first time, stinging nettle utilized a outstanding antimicrobial-activity pathogenic bacterial fungal strains. HepG2 cancerous cell without normal line. Finally, via potential antibacterial, antifungal, action, making them useful medical field.

Язык: Английский

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Plasmids, a molecular cornerstone of antimicrobial resistance in the One Health era

Nature Reviews Microbiology, Год журнала: 2023, Номер 22(1), С. 18 - 32

Опубликована: Июль 10, 2023

Язык: Английский

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Bio-fabrication of Selenium Nanoparticles Using Baker’s Yeast Extract and Its Antimicrobial Efficacy on Food Borne Pathogens

Applied Biochemistry and Biotechnology, Год журнала: 2022, Номер 194(5), С. 1898 - 1910

Опубликована: Янв. 7, 2022

Язык: Английский

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The Mechanism of Bacterial Resistance and Potential Bacteriostatic Strategies

Antibiotics, Год журнала: 2022, Номер 11(9), С. 1215 - 1215

Опубликована: Сен. 8, 2022

Bacterial drug resistance is rapidly developing as one of the greatest threats to human health. Bacteria will adopt corresponding strategies crack inhibitory effect antibiotics according antibacterial mechanism antibiotics, involving mutation target, secreting hydrolase, and discharging out cells through an efflux pump, etc. In recent years, bacteria are found constantly evolve new mechanisms including target protective protein, changes in cell morphology, so on, endowing them with multiple defense systems against leading emergence multi-drug resistant (MDR) unavailability drugs clinics. Correspondingly, researchers attempt uncover mystery bacterial develop more convenient effective strategies. Although traditional still play a significant role treatment diseases caused by sensitive pathogenic bacteria, they gradually lose efficacy MDR bacteria. Therefore, highly compounds, such phage therapy CRISPER-Cas precision therapy, gaining increasing amount attention, considered be treatments moist potential regard future. this review, nine identified summarized, which enhance retention rate under action promote distribution drug-resistant (DRB) population. Afterwards, three kinds methods introduced, compounds exhibit broad application prospects different mechanisms, has been successfully applied infectious super technology can edit genes at gene level, high accuracy flexibility. These provide options for clinical treatment, greatly alleviate current crisis.

Язык: Английский

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Microbial communities form rich extracellular metabolomes that foster metabolic interactions and promote drug tolerance

Nature Microbiology, Год журнала: 2022, Номер 7(4), С. 542 - 555

Опубликована: Март 21, 2022

Abstract Microbial communities are composed of cells varying metabolic capacity, and regularly include auxotrophs that lack essential pathways. Through analysis for amino acid biosynthesis pathways in microbiome data derived from >12,000 natural microbial obtained as part the Earth Microbiome Project (EMP), study auxotrophic–prototrophic interactions self-establishing metabolically cooperating yeast (SeMeCos), we reveal a imprinted mechanism links presence to an increase gains antimicrobial drug tolerance. As consequence adaptations necessary uptake specific metabolites, obtain altered flux distributions, export more metabolites and, this way, enrich community environments metabolites. Moreover, increased efflux activities reduce intracellular concentrations, allowing grow levels above minimal inhibitory concentrations. For example, show antifungal action azoles is greatly diminished enriched environment. Our results hence provide explains why robust exposure when they interact metabolically.

Язык: Английский

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Cross-protection and cross-feeding between Klebsiella pneumoniae and Acinetobacter baumannii promotes their co-existence

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Фев. 9, 2023

Acinetobacter baumannii and Klebsiella pneumoniae are opportunistic pathogens frequently co-isolated from polymicrobial infections. The infections where these co-exist can be more severe recalcitrant to therapy than caused by either species alone, however there is a lack of knowledge on their potential synergistic interactions. In this study we characterise the genomes A. K. strains single human lung infection. We examine various aspects interactions through transcriptomic, phenomic phenotypic assays that form basis for understanding effects antimicrobial resistance virulence during co-infection. Using co-culturing analyses secreted metabolites, discover ability cross-feed by-products sugar fermentation. Minimum inhibitory concentration testing mono- co-cultures reveals cross-protect against cephalosporin, cefotaxime. Our demonstrates distinct syntrophic occur between pneumoniae, helping elucidate co-existence in

Язык: Английский

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Convergence of resistance and evolutionary responses in Escherichia coli and Salmonella enterica co-inhabiting chicken farms in China

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Янв. 5, 2024

Abstract Sharing of genetic elements among different pathogens and commensals inhabiting same hosts environments has significant implications for antimicrobial resistance (AMR), especially in settings with high exposure. We analysed 661 Escherichia coli Salmonella enterica isolates collected within across environments, 10 Chinese chicken farms over 2.5 years using data-mining methods. Most possessed the clinically relevant AMR-carrying mobile (plasmids: 70.6%, transposons: 78%), which also showed recent common evolution. Supervised machine learning classifiers revealed known novel AMR-associated mutations genes underlying to 28 antimicrobials, primarily associated E. susceptibility S. . Many were essential affected metabolic processes both species, albeit varying degrees phylogenetic penetration. Multi-modal strategies are crucial investigate interplay mobilome, metabolism cohabiting bacteria, ecological where community-driven selection occurs.

Язык: Английский

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An effective antibiofilm strategy based on bacteriophages armed with silver nanoparticles

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Апрель 20, 2024

Abstract The emerging antibiotic resistance in pathogenic bacteria is a key problem modern medicine that has led to search for novel therapeutic strategies. A potential approach managing such involves the use of their natural killers, namely lytic bacteriophages. Another effective method metal nanoparticles with antimicrobial properties. However, phages armed as an strategy, particularly respect biofilms, remains unexplored. Here, we show T7 silver exhibit greater efficacy terms controlling bacterial biofilm, compared or alone. We initially identified nanoparticle-binding peptide, then constructed successfully displayed peptide on outer surface viral head. These recombinant, AgNP-binding could effectively eradicate even when used at low concentrations. Additionally, concentrations were not toxic eukaryotic cells. Our results combination phage-bound effective, synergistic and safe strategy treatment biofilms.

Язык: Английский

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Unraveling resistance mechanisms in combination therapy: A comprehensive review of recent advances and future directions

Heliyon, Год журнала: 2024, Номер 10(6), С. e27984 - e27984

Опубликована: Март 1, 2024

Antimicrobial resistance is a global health threat. Misuse and overuse of antimicrobials are the main drivers in developing drug-resistant bacteria. The emergence rapid spread multi-resistant bacteria requires urgent multisectoral action to generate novel treatment alternatives. Combination therapy offers potential exploit synergistic effects for enhanced antibacterial efficacy drugs. Understanding complex dynamics kinetics drug interactions combination crucial. Therefore, this review outlines current advances antibiotic resistance's evolutionary genetic therapies-exposed Moreover, we also discussed four pivotal future research areas comprehend better development treated with strategies.

Язык: Английский

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Effect of antibiotics on diverse aquatic plants in aquatic ecosystems

Aquatic Toxicology, Год журнала: 2025, Номер 281, С. 107289 - 107289

Опубликована: Фев. 22, 2025

Язык: Английский

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