Pharmaceutics,
Год журнала:
2025,
Номер
17(2), С. 182 - 182
Опубликована: Фев. 1, 2025
Background:
Colistin,
a
lipopeptide
antibiotic
usually
used
as
last
resort
against
multidrug-resistant
bacterial
strains,
has
also
begun
to
address
the
challenge
of
antimicrobial
resistance.
Objective:
this
study
evaluates
whether
hybrid
nanoparticles
(HNPs)
composed
Phospholipon®
90G,
cholesterol,
and
colistin
can
enhance
its
effectiveness
resistant
clinical
isolates
Klebsiella
pneumoniae,
clinically
significant
Gram-negative
bacterium.
Methods:
HNPs
were
developed
using
ethanol
injection
method
coated
with
chitosan
through
layer-by-layer
technique.
HNP
characterization
included
measurements
particle
size,
polydispersity
index
(PDI),
zeta
potential,
along
thermal
(DSC)
spectrophotometric
(FT-IR)
analyses.
Ultrafiltration
ATR-FTIR
employed
assess
colistin’s
association
release
efficiencies.
The
biological
evaluation
followed
CLSI
guidelines.
Results:
uncoated
(U-HNP)
chitosan-coated
(Ch-HNP)
described
monodisperse
populations,
respective
PDI
values
~0.124
~0.150,
Z-averages
~249
nm
~250
nm,
potential
+17
mV
+20
mV.
Colistin’s
efficiencies
approximately
79%
10%,
respectively.
Regarding
activity,
results
showed
that
part
is
poorly
effective
microorganism.
However,
in
most
strain,
activity
increased
slightly
when
was
chitosan.
Conclusions:
high
stability
disaggregation,
limiting
and,
therefore,
affecting
performance.
Pharmaceutics,
Год журнала:
2023,
Номер
15(4), С. 1116 - 1116
Опубликована: Март 31, 2023
Multidrug-resistant
(MDR)
bacteria
are
rapidly
emerging,
coupled
with
the
failure
of
current
antibiotic
therapy;
thus,
new
alternatives
for
effectively
treating
infections
caused
by
MDR
required.
Hyperthermia-mediated
photothermal
therapy
(PTT)
and
reactive
oxygen
species
(ROS)-mediated
photodynamic
(PDT)
have
attracted
extensive
attention
as
antibacterial
therapies
owing
to
advantages
such
low
invasiveness,
toxicity,
likelihood
causing
bacterial
resistance.
However,
both
strategies
notable
drawbacks,
including
high
temperature
requirements
PTT
weak
ability
PDT-derived
ROS
penetrate
target
cells.
To
overcome
these
limitations,
a
combination
PDT
has
been
used
against
bacteria.
In
this
review,
we
discuss
unique
benefits
limitations
The
mechanisms
underlying
synergistic
effects
PTT–PDT
also
discussed.
Furthermore,
introduced
advancements
in
methods
using
nano-based
agents
treat
Finally,
highlight
existing
challenges
future
perspectives
We
believe
that
review
will
encourage
PTT-
PDT-based
research
can
be
referenced
clinical
applications.
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2023,
Номер
13
Опубликована: Дек. 20, 2023
The
rise
of
multi-drug
resistant
(MDR)
pathogens
poses
a
significant
challenge
to
the
field
infectious
disease
treatment.
To
overcome
this
problem,
novel
strategies
are
being
explored
enhance
effectiveness
antibiotics.
Antibiotic
adjuvants
have
emerged
as
promising
approach
combat
MDR
by
acting
synergistically
with
This
review
focuses
on
role
antibiotic
synergistic
tool
in
fight
against
pathogens.
Adjuvants
refer
compounds
or
agents
that
activity
antibiotics,
either
potentiating
their
effects
targeting
mechanisms
resistance.
utilization
offers
several
advantages.
Firstly,
they
can
restore
existing
antibiotics
strains.
inhibit
confer
resistance,
making
susceptible
action
Secondly,
improving
penetration
into
bacterial
cells,
increasing
stability,
inhibiting
efflux
pumps
expel
from
cells.
Various
types
been
investigated,
including
pump
inhibitors,
resistance-modifying
agents,
and
disrupt
biofilms.
These
act
resulting
increased
antibacterial
overcoming
resistance
mechanisms.
In
conclusion,
potential
revolutionize
treatment
By
enhancing
efficacy
offer
strategy
growing
threat
Further
research
development
crucial
harness
full
bring
them
closer
clinical
application.
Antibiotics,
Год журнала:
2024,
Номер
13(3), С. 271 - 271
Опубликована: Март 18, 2024
Infectious
diseases
are
a
significant
challenge
to
global
healthcare,
especially
in
the
face
of
increasing
antibiotic
resistance.
This
urgent
issue
requires
continuous
exploration
and
development
new
antimicrobial
drugs.
In
this
regard,
secondary
metabolites
derived
from
endophytic
microorganisms
stand
out
as
promising
sources
for
finding
antimicrobials.
Endophytic
microorganisms,
residing
within
internal
tissues
plants,
have
demonstrated
capacity
produce
diverse
bioactive
compounds
with
substantial
pharmacological
potential.
Therefore,
numerous
been
isolated
endophytes,
particularly
fungi
actinomycetes.
However,
only
limited
number
these
subjected
comprehensive
studies
regarding
their
mechanisms
action
against
bacterial
cells.
Furthermore,
investigation
effects
on
antibiotic-resistant
bacteria
identification
biosynthetic
gene
clusters
responsible
synthesizing
conducted
subset
compounds.
Through
analysis
current
research
findings,
review
describes
drugs
antibacterial
activities
natural
endophytes
bacteria,
synthesis
metabolites.
Heliyon,
Год журнала:
2025,
Номер
11(2), С. e42013 - e42013
Опубликована: Янв. 1, 2025
The
issue
of
antimicrobial
resistance
(AMR)
poses
a
major
challenge
to
global
health,
evidenced
by
alarming
mortality
predictions
and
the
diminishing
efficiency
conventional
drugs.
CRISPR-Cas
system
has
proven
be
powerful
tool
in
addressing
this
challenge.
It
originated
from
bacterial
adaptive
immune
mechanisms
gained
significant
recognition
scientific
community.
This
review
aims
explore
applications
technologies
combating
AMR,
evaluating
their
effectiveness,
challenges,
potential
for
integration
into
current
strategies.
We
conducted
comprehensive
recent
literature
databases
such
as
PubMed
Web
Science,
focusing
on
studies
that
employ
against
AMR.
offer
transformative
approach
combat
with
extend
beyond
traditional
Integrating
these
existing
methods
could
significantly
enhance
our
ability
manage
potentially
reverse
growing
problem
resistance.
Future
research
should
address
technical
ethical
barriers
facilitate
safe
effective
clinical
environmental
applications.
underscores
necessity
interdisciplinary
collaboration
international
cooperation
harness
full
fight
superbugs.
Heliyon,
Год журнала:
2023,
Номер
9(2), С. e13457 - e13457
Опубликована: Фев. 1, 2023
Heavy
metal
co-resistance
with
antibiotics
appears
to
be
synergistic
in
bacterial
isolates
via
similar
mechanisms.
This
synergy
has
the
potential
amplify
resistance
genes
environment
which
can
transferred
into
clinical
settings.
The
aim
of
this
study
was
assess
heavy
metals
bacteria
from
dumpsite
addition
physicochemical
analysis.
Sample
collection,
analysis,
and
enumeration
total
heterotrophic
counts
(THBC)
were
all
carried
out
using
standard
existing
protocols.
Identified
subjected
sensitivity
test
Kirby
Bauer
disc
diffusion
technique
resulting
multidrug
resistant
(MDR)
tolerance
agar
dilution
increasing
concentrations
(50,
100,
150,
200
250
μg/ml)
our
metals.
THBC
ranged
6.68
7.92
×
105
cfu/g.
Out
20
sensitivity,
50%
(n
=
10)
showed
multiple
drug
these
B.
subtilis,
cereus,
C.
freundii,
P.
aeruginosa,
Enterobacter
sp,
E.
coli
5).
At
lowest
concentration
(50
μg/ml),
MDR
tolerated
metals,
but
at
μg/ml,
apart
cadmium
lead,
100%
sensitive
chromium,
vanadium
cobalt.
control
isolate
only
cobalt
chromium
50
other
level
shown
by
is
a
call
for
concern.
ACS Applied Materials & Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 6, 2025
Antibiotic
resistance
has
become
a
critical
health
crisis
globally.
Traditional
strategies
using
antibiotics
can
lead
to
drug-resistance,
while
inorganic
antimicrobial
agents
cause
severe
systemic
toxicity.
Here,
we
have
developed
dual-antibiotic
hydrogel
delivery
system
(PDA-Ag@Levo/CMCS),
which
achieve
controlled
release
of
clinical
levofloxacin
(Levo)
and
classic
nanoscale
antibiotic
silver
nanoparticles
(AgNPs),
effectively
eliminating
drug-resistant
P.
aeruginosa.
Benefiting
from
the
photothermal
(PTT)
effect
polydopamine
(PDA),
local
high
temperature
generated
by
PDA-Ag@Levo/CMCS
quickly
kill
bacteria
through
continuous
responsive
dual-antibiotics
restore
sensitivity
ineffective
antibiotics.
Moreover,
AgNPs
could
significantly
improve
efficiency
traditional
disrupting
bacterial
membranes
reducing
their
toxicity
healthy
tissues.
A
clever
combination
PTT
drug-combination
therapy
eliminate
biofilms
bacteria.
Mechanism
studies
shown
that
PDA-Ag@Levo
might
aeruginosa
biofilm
formation
protein
synthesis,
inhibit
mutation
promoting
expression
related
genes,
such
as
rpoS,
dinB,
mutS.
Collectively,
synergistic
this
combined
with
provides
creative
strategy
for
in
chronic
infection
wounds.
