Specific Enrichment of arsM-Carrying Microorganisms with Nitrogen Fixation and Dissimilatory Nitrate Reduction Function Enhances Arsenic Methylation in Plant Rhizosphere Soil

Environmental Science & Technology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 14, 2025

Plants can recruit microorganisms to enhance soil arsenic (As) removal and nitrogen (N) turnover, but how microbial As methylation in the rhizosphere is affected by N biotransformation not well understood. Here, we used acetylene reduction assay, arsM gene amplicon, metagenome sequencing evaluate influence of on Vetiveria zizanioides, a potential hyperaccumulator. V. zizanioides was grown mining soils (MS) artificial As-contaminated (AS) over two generations controlled pot experiment. Results showed that content dimethylarsinic acid significantly positively correlated with rate fixation activity nitrite reductase. The As-methylating species (e.g., Flavisolibacter Paraflavitalea) were enriched root-associated compartments second generation MS AS. Notably, higher abundance genes involved (nifD, nifK) dissimilatory nitrate ammonium (narG/H, nirB/D/K/S) detected than first generation. metabolic pathway analysis further demonstrated fixing-stimulative DNRA-stimulative could provide synthesis S-adenosyl-l-methionine, serving as methyl donors for methylation. This study highlights important conversion-stimulative pathways has implications enhancing phytoremediation soils.

Язык: Английский

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Adaptive modification of antiviral defense systems in microbial community under Cr-induced stress

Microbiome, Год журнала: 2025, Номер 13(1)

Опубликована: Янв. 31, 2025

The prokaryotic antiviral defense systems are crucial for mediating prokaryote-virus interactions that influence microbiome functioning and evolutionary dynamics. Despite the prevalence significance of systems, their responses to abiotic stress ecological consequences remain poorly understood in soil ecosystems. We established microcosm with varying concentrations hexavalent chromium (Cr(VI)) investigate adaptive modifications under stress. Utilizing hybrid metagenomic assembly long-read short-read sequencing, we discovered were more diverse prevalent heavily polluted soils, which was corroborated by meta-analyses public datasets from various heavy metal-contaminated sites. As Cr(VI) concentration increased, prokaryotes favoring mutualism gradually supplanted those incurring high costs. Additionally, as enriched exhibited synchronization microbial metal resistance genes. Furthermore, proportion carried mobile genetic elements (MGEs), including plasmids viruses, increased approximately 43% 39%, respectively, rising Cr concentrations. This trend is conducive strengthening dissemination sharing resources within communities. Overall, our study reveals modification ecosystems stress, well positive contributions establishing evolution resistance. These findings advance understanding adaptation stressful environments may inspire novel approaches manipulation bioremediation. Video Abstract.

Язык: Английский

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Organophosphorus mineralizing-Streptomyces species underpins uranate immobilization and phosphorus availability in uranium tailings

Journal of Hazardous Materials, Год журнала: 2024, Номер 476, С. 134975 - 134975

Опубликована: Июнь 19, 2024

Язык: Английский

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Differential Mechanisms of Microbial As(III) and Sb(III) Oxidation and Their Contribution to Tailings Reclamation

Environmental Science & Technology, Год журнала: 2024, Номер 58(26), С. 11447 - 11458

Опубликована: Июнь 20, 2024

Mine tailings are extremely oligotrophic environments frequently contaminated with elevated As and Sb, making As(III) Sb(III) oxidation potentially important energy sources for the tailing microbiome. Although they have been proposed to share similar metabolic pathways, a systemic comparison of mechanisms utilization efficiencies requires further elucidation. In this study, we employed combination physicochemical, molecular, bioinformatic analyses compare kinetic genetic as well their respective fueling key nutrient acquisition metabolisms. Thiobacillus Rhizobium spp. were identified functional populations both in mine by DNA-stable isotope probing. However, these microorganisms mediated via different resulting preferential over As(III). Notably, can facilitate nitrogen fixation phosphate solubilization tailings, being more efficient powering processes. Thus, study provided novel insights into microbial efficiencies, which may be critical reclamation tailings.

Язык: Английский

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Global distribution pattern of soil phosphorus‐cycling microbes under the influence of human activities

Global Change Biology, Год журнала: 2024, Номер 30(8)

Опубликована: Авг. 1, 2024

Human activities have profoundly altered the Earth's phosphorus (P) cycling process and its associated microbial communities, yet their global distribution pattern response to human influences remain unclear. Here, we estimated abundances of P-cycling genes from 3321 soil metagenomic samples mapped five key processes, that is, organic phosphoester hydrolysis, inorganic solubilization, two-component system, phosphotransferase transporters. Structural equation modeling random forest analysis were employed assess impact anthropogenic environmental factors on abundance genes. Our findings suggest although less significant than climate profile, human-related factors, such as economic population, are important drivers for variations in gene abundance. Notably, increased parallel extent intervention, but generally at low moderate levels activities. Furthermore, identified critical genera, Pseudomonas Lysobacter, which sensitive changes This study provides insights into responses microbes a scale, enhancing our understanding P underscoring importance sustainable biogeochemical cycle.

Язык: Английский

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The influences of heavy metals on soil microbial C, N, P cycling and heavy metal resistance under different fertilization regimes

Environmental Pollution, Год журнала: 2025, Номер unknown, С. 125915 - 125915

Опубликована: Фев. 1, 2025

Язык: Английский

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Long-Term Organic Fertilization Strengthens the Soil Phosphorus Cycle and Phosphorus Availability by Regulating the pqqC- and phoD-Harboring Bacterial Communities

Microbial Ecology, Год журнала: 2023, Номер 86(4), С. 2716 - 2732

Опубликована: Авг. 2, 2023

Язык: Английский

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Biochar and nano-hydroxyapatite combined remediation of soil surrounding tailings area: multi-metal(loid)s fixation and soybean rhizosphere soil microbial improvement

Journal of Hazardous Materials, Год журнала: 2024, Номер 469, С. 133817 - 133817

Опубликована: Фев. 17, 2024

Язык: Английский

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Ecological succession of abundant and rare subcommunities during aerobic composting in the presence of residual amoxicillin

Journal of Hazardous Materials, Год журнала: 2024, Номер 465, С. 133456 - 133456

Опубликована: Янв. 6, 2024

Язык: Английский

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The adsorption and fixation of Cd and Pb by the microbial consortium weakened the toxic effect of heavy metal-contaminated soil on rice

Chemical Engineering Journal, Год журнала: 2024, Номер 497, С. 154684 - 154684

Опубликована: Авг. 10, 2024

Язык: Английский

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