Combining NeuroPainting with transcriptomics reveals cell-type-specific morphological and molecular signatures of the 22q11.2 deletion DOI Creative Commons
Matthew Tegtmeyer,

Dhara Liyanage,

Yu Han

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 17, 2024

Abstract Neuropsychiatric conditions pose substantial challenges for therapeutic development due to their complex and poorly understood underlying mechanisms. High-throughput, unbiased phenotypic assays present a promising path advancing discovery, especially within disease-relevant neural tissues. Here, we introduce NeuroPainting, novel adaptation of the Cell Painting assay, optimized high-dimensional morphological phenotyping cell types, including neurons, neuronal progenitor cells, astrocytes derived from human stem cells. Using quantified structure organelle behavior across various brain creating public dataset over 4,000 cellular traits. This extensive not only sets new benchmark screening in neuropsychiatric research but also serves as gold standard community, enabling comparisons validation results. We then applied NeuroPainting identify signatures associated with 22q11.2 deletion, major genetic risk factor schizophrenia. observed profound cell-type-specific effects significant alterations mitochondrial structure, endoplasmic reticulum organization, cytoskeletal dynamics, particularly astrocytes. Transcriptomic analysis revealed reduced expression adhesion genes deletion astrocytes, consistent recent post-mortem findings. Integrating RNA sequencing data profiles uncovered biological link between altered specific molecules changes morphology These findings underscore power combined phenomic transcriptomic analyses reveal mechanistic insights variants conditions.

Язык: Английский

GPCRs identified on mitochondrial membranes: New therapeutic targets for diseases. DOI Creative Commons
Y. Pan, Ning Ji, Lu Jiang

и другие.

Journal of Pharmaceutical Analysis, Год журнала: 2025, Номер unknown, С. 101178 - 101178

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Fluoride induces spermatocyte apoptosis by IP3R1/MCU-mediated mitochondrial calcium overload through MAMs DOI
Xin Guo, Linyuan Wang,

Jingyan Xuan

и другие.

Journal of Hazardous Materials, Год журнала: 2025, Номер 489, С. 137514 - 137514

Опубликована: Фев. 5, 2025

Язык: Английский

Процитировано

1
Recent development of mitochondrial metabolism and dysfunction in osteoarthritis DOI Creative Commons
Pengchao Guo, Ahmad Alhaskawi, Safwat Adel Abdo Moqbel

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Фев. 13, 2025

Osteoarthritis is a degenerative joint disorder characterized by cartilage degradation, synovial inflammation, and altered subchondral bone structure. Recent insights have identified mitochondrial dysfunction as pivotal factor in OA pathogenesis, contributing to chondrocyte apoptosis, oxidative stress, extracellular matrix degradation. Disruptions dynamics, including impaired biogenesis, mitophagy, metabolic shifts from phosphorylation glycolysis, exacerbate damage promoting the production of reactive oxygen species matrix-degrading enzymes such ADAMTS MMPs. This review explores molecular mechanisms underlying OA, emphasizing its role homeostasis inflammation. Furthermore, it highlights emerging therapeutic strategies targeting pathways, antioxidants, mitophagy enhancers, modulators, potential interventions mitigate disease progression, which offer promising avenues for advancing personalized disease-modifying treatments OA.

Язык: Английский

Процитировано

1
Downregulation of HSP47 Triggers ER Stress-mediated Apoptosis of Hypertrophic Chondrocytes Contributing to T-2 toxin-induced Cartilage Damage DOI
Meng Zhang, Yinan Liu, Hui Wang

и другие.

Environmental Pollution, Год журнала: 2025, Номер unknown, С. 125640 - 125640

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Neuroprotective Role of Da Qin Jiu Decoction in Ischemic Stroke: Mitochondrial Rescue through PI3K/Akt-Mediated UPRmt Activation DOI
Jing Luo,

Yaling Zheng,

Jialei Chen

и другие.

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119433 - 119433

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Zhizichi decoction alleviates depressive-like behaviors through modulating mitochondria-associated membrane via the IP3R3-GRP75-VDAC1 complex DOI
Ye Liu,

Zicheng Zhang,

Yimeng Zhao

и другие.

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119628 - 119628

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Cornuside as a promising therapeutic agent for diabetic kidney disease: Targeting regulation of Ca2+ disorder-mediated renal tubular epithelial cells apoptosis DOI

Gai Gao,

Xuan Su, Shu-Yan Liu

и другие.

International Immunopharmacology, Год журнала: 2025, Номер 149, С. 114190 - 114190

Опубликована: Фев. 3, 2025

Язык: Английский

Процитировано

0
Mitochondria-associated membranes (MAMs) in age-related heart diseases, role of endoplasmic reticulum stress DOI
Alejandro Silva‐Palacios, Zeltzin Alejandra Ceja-Galicia, Alejandra Zúñiga-Muñoz

и другие.

Advances in pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Innovative engineering of superoxide dismutase for enhanced cardioprotective biocatalysis in myocardial ischemia-reperfusion injury DOI
Bo Zhao,

Jianjun Peng,

C. Chen

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 286, С. 137656 - 137656

Опубликована: Ноя. 28, 2024

Язык: Английский

Процитировано

2
Combining NeuroPainting with transcriptomics reveals cell-type-specific morphological and molecular signatures of the 22q11.2 deletion DOI Creative Commons
Matthew Tegtmeyer,

Dhara Liyanage,

Yu Han

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 17, 2024

Abstract Neuropsychiatric conditions pose substantial challenges for therapeutic development due to their complex and poorly understood underlying mechanisms. High-throughput, unbiased phenotypic assays present a promising path advancing discovery, especially within disease-relevant neural tissues. Here, we introduce NeuroPainting, novel adaptation of the Cell Painting assay, optimized high-dimensional morphological phenotyping cell types, including neurons, neuronal progenitor cells, astrocytes derived from human stem cells. Using quantified structure organelle behavior across various brain creating public dataset over 4,000 cellular traits. This extensive not only sets new benchmark screening in neuropsychiatric research but also serves as gold standard community, enabling comparisons validation results. We then applied NeuroPainting identify signatures associated with 22q11.2 deletion, major genetic risk factor schizophrenia. observed profound cell-type-specific effects significant alterations mitochondrial structure, endoplasmic reticulum organization, cytoskeletal dynamics, particularly astrocytes. Transcriptomic analysis revealed reduced expression adhesion genes deletion astrocytes, consistent recent post-mortem findings. Integrating RNA sequencing data profiles uncovered biological link between altered specific molecules changes morphology These findings underscore power combined phenomic transcriptomic analyses reveal mechanistic insights variants conditions.

Язык: Английский

Процитировано

1