Combining NeuroPainting with transcriptomics reveals cell-type-specific morphological and molecular signatures of the 22q11.2 deletion DOI Creative Commons
Matthew Tegtmeyer,

Dhara Liyanage,

Yu Han

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

Abstract Neuropsychiatric conditions pose substantial challenges for therapeutic development due to their complex and poorly understood underlying mechanisms. High-throughput, unbiased phenotypic assays present a promising path advancing discovery, especially within disease-relevant neural tissues. Here, we introduce NeuroPainting, novel adaptation of the Cell Painting assay, optimized high-dimensional morphological phenotyping cell types, including neurons, neuronal progenitor cells, astrocytes derived from human stem cells. Using quantified structure organelle behavior across various brain creating public dataset over 4,000 cellular traits. This extensive not only sets new benchmark screening in neuropsychiatric research but also serves as gold standard community, enabling comparisons validation results. We then applied NeuroPainting identify signatures associated with 22q11.2 deletion, major genetic risk factor schizophrenia. observed profound cell-type-specific effects significant alterations mitochondrial structure, endoplasmic reticulum organization, cytoskeletal dynamics, particularly astrocytes. Transcriptomic analysis revealed reduced expression adhesion genes deletion astrocytes, consistent recent post-mortem findings. Integrating RNA sequencing data profiles uncovered biological link between altered specific molecules changes morphology These findings underscore power combined phenomic transcriptomic analyses reveal mechanistic insights variants conditions.

Language: Английский

GPCRs identified on mitochondrial membranes: New therapeutic targets for diseases. DOI Creative Commons
Y. Pan, Ning Ji, Lu Jiang

et al.

Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101178 - 101178

Published: Jan. 1, 2025

Language: Английский

Citations

1
Fluoride induces spermatocyte apoptosis by IP3R1/MCU-mediated mitochondrial calcium overload through MAMs DOI
Xin Guo, Linyuan Wang,

Jingyan Xuan

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 489, P. 137514 - 137514

Published: Feb. 5, 2025

Language: Английский

Citations

1
Recent development of mitochondrial metabolism and dysfunction in osteoarthritis DOI Creative Commons
Pengchao Guo, Ahmad Alhaskawi, Safwat Adel Abdo Moqbel

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 13, 2025

Osteoarthritis is a degenerative joint disorder characterized by cartilage degradation, synovial inflammation, and altered subchondral bone structure. Recent insights have identified mitochondrial dysfunction as pivotal factor in OA pathogenesis, contributing to chondrocyte apoptosis, oxidative stress, extracellular matrix degradation. Disruptions dynamics, including impaired biogenesis, mitophagy, metabolic shifts from phosphorylation glycolysis, exacerbate damage promoting the production of reactive oxygen species matrix-degrading enzymes such ADAMTS MMPs. This review explores molecular mechanisms underlying OA, emphasizing its role homeostasis inflammation. Furthermore, it highlights emerging therapeutic strategies targeting pathways, antioxidants, mitophagy enhancers, modulators, potential interventions mitigate disease progression, which offer promising avenues for advancing personalized disease-modifying treatments OA.

Language: Английский

Citations

1
Downregulation of HSP47 Triggers ER Stress-mediated Apoptosis of Hypertrophic Chondrocytes Contributing to T-2 toxin-induced Cartilage Damage DOI
Meng Zhang, Yinan Liu, Hui Wang

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 125640 - 125640

Published: Jan. 1, 2025

Language: Английский

Citations

0
Neuroprotective Role of Da Qin Jiu Decoction in Ischemic Stroke: Mitochondrial Rescue through PI3K/Akt-Mediated UPRmt Activation DOI
Jing Luo,

Yaling Zheng,

Jialei Chen

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119433 - 119433

Published: Feb. 1, 2025

Language: Английский

Citations

0
Zhizichi decoction alleviates depressive-like behaviors through modulating mitochondria-associated membrane via the IP3R3-GRP75-VDAC1 complex DOI
Ye Liu,

Zicheng Zhang,

Yimeng Zhao

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119628 - 119628

Published: March 1, 2025

Language: Английский

Citations

0
Cornuside as a promising therapeutic agent for diabetic kidney disease: Targeting regulation of Ca2+ disorder-mediated renal tubular epithelial cells apoptosis DOI

Gai Gao,

Xuan Su, Shu-Yan Liu

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 149, P. 114190 - 114190

Published: Feb. 3, 2025

Language: Английский

Citations

0
Mitochondria-associated membranes (MAMs) in age-related heart diseases, role of endoplasmic reticulum stress DOI
Alejandro Silva‐Palacios, Zeltzin Alejandra Ceja-Galicia, Alejandra Zúñiga-Muñoz

et al.

Advances in pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Innovative engineering of superoxide dismutase for enhanced cardioprotective biocatalysis in myocardial ischemia-reperfusion injury DOI
Bo Zhao,

Jianjun Peng,

C. Chen

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 286, P. 137656 - 137656

Published: Nov. 28, 2024

Language: Английский

Citations

2
Combining NeuroPainting with transcriptomics reveals cell-type-specific morphological and molecular signatures of the 22q11.2 deletion DOI Creative Commons
Matthew Tegtmeyer,

Dhara Liyanage,

Yu Han

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

Abstract Neuropsychiatric conditions pose substantial challenges for therapeutic development due to their complex and poorly understood underlying mechanisms. High-throughput, unbiased phenotypic assays present a promising path advancing discovery, especially within disease-relevant neural tissues. Here, we introduce NeuroPainting, novel adaptation of the Cell Painting assay, optimized high-dimensional morphological phenotyping cell types, including neurons, neuronal progenitor cells, astrocytes derived from human stem cells. Using quantified structure organelle behavior across various brain creating public dataset over 4,000 cellular traits. This extensive not only sets new benchmark screening in neuropsychiatric research but also serves as gold standard community, enabling comparisons validation results. We then applied NeuroPainting identify signatures associated with 22q11.2 deletion, major genetic risk factor schizophrenia. observed profound cell-type-specific effects significant alterations mitochondrial structure, endoplasmic reticulum organization, cytoskeletal dynamics, particularly astrocytes. Transcriptomic analysis revealed reduced expression adhesion genes deletion astrocytes, consistent recent post-mortem findings. Integrating RNA sequencing data profiles uncovered biological link between altered specific molecules changes morphology These findings underscore power combined phenomic transcriptomic analyses reveal mechanistic insights variants conditions.

Language: Английский

Citations

1