Genetically Engineered-MSC Therapies for Non-unions, Delayed Unions and Critical-size Bone Defects

International Journal of Molecular Sciences, Год журнала: 2019, Номер 20(14), С. 3430 - 3430

Опубликована: Июль 12, 2019

The normal bone regeneration process is a complex and coordinated series of events involving different cell types molecules. However, this impaired in critical-size/large defects, with non-unions or delayed unions remaining major clinical problem. Novel strategies are needed to aid the current therapeutic approaches. Mesenchymal stem/stromal cells (MSCs) able promote regeneration. Their beneficial effects can be improved by modulating expression levels specific genes purpose stimulating MSC proliferation, osteogenic differentiation their immunomodulatory capacity. In context, genetic engineering MSCs expected further enhance pro-regenerative properties accelerate healing. Herein, we review most promising molecular candidates (protein-coding non-coding transcripts) discuss methodologies engineer deliver MSCs, mainly focusing on vivo animal studies. Considering potential secretome for repair, topic has also been addressed. Furthermore, results studies using discussed. Finally, debate advantages limitations genetically-engineered as promoters fracture regeneration/repair.

Язык: Английский

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Lactobacillus‐fermented milk products attenuate bone loss in an experimental rat model of ovariectomy‐induced post‐menopausal primary osteoporosis

Journal of Applied Microbiology, Год журнала: 2020, Номер 130(6), С. 2041 - 2062

Опубликована: Сен. 25, 2020

In this study, we investigated the anti‐osteoporotic effect of two fermented milk products (FMPs) by Lactobacillus plantarum A41 and fermentum SRK414 on a rat model ovariectomy‐induced post‐menopausal primary osteoporosis. The FMPs increased bone volume mineral density (BMD) in ovariectomized (OVX) rats, normalized biomarkers serum. Additionally, they altered gene expression levels bone‐metabolism‐related markers. Furthermore, downregulated bone‐apoptosis‐related genes stimulated ovariectomy. Interestingly, decreased inflammation markers serum, bone, ileum colon rats. Gut bacterial populations were also affected upon FMP treatment due to increase abundance genus Faecalibacterium prausnitzii. Milk L. can exhibit effects osteoporosis via regulating used study be an ideal method that has its potential treating instead drug treatments.

Язык: Английский

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Alteration of circRNA and lncRNA expression profile in exosomes derived from periodontal ligament stem cells undergoing osteogenic differentiation

Archives of Oral Biology, Год журнала: 2020, Номер 121, С. 104984 - 104984

Опубликована: Ноя. 11, 2020

Язык: Английский

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Small and Long Non-coding RNAs as Functional Regulators of Bone Homeostasis, Acting Alone or Cooperatively

Molecular Therapy — Nucleic Acids, Год журнала: 2020, Номер 21, С. 792 - 803

Опубликована: Июль 15, 2020

Emerging knowledge indicates that non-coding RNAs, including microRNAs (miRNAs) and long-noncoding RNAs (lncRNAs), have a pivotal role in bone development the pathogenesis of bone-related disorders. Most recently, miRNAs started to be regarded as potential biomarkers or targets for various sets diseases, while lncRNAs gained attention new layer gene expression control acting through versatile interactions, also with miRNAs. The rapid RNA sequencing techniques based on next-generation (NGS) gives us better insight into molecular pathways regulated by miRNA-lncRNA network. In this review, we summarize current related function regulators genes are crucial proper metabolism homeostasis. We characterized important their signatures, relationship bone. Analysis biological lncRNAs, well network, will pave way understanding think may lead innovative diagnostic tools therapeutic approaches

Язык: Английский

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miR-99a in bone homeostasis: Regulating osteogenic lineage commitment and osteoclast differentiation

Bone, Год журнала: 2020, Номер 134, С. 115303 - 115303

Опубликована: Фев. 29, 2020

Язык: Английский

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