Bone, Год журнала: 2024, Номер unknown, С. 117382 - 117382
Опубликована: Дек. 1, 2024
Язык: Английский
Advanced Materials, Год журнала: 2025, Номер unknown
Опубликована: Фев. 5, 2025
Abstract Osteoporosis, characterized by low bone mass and high fracture risk, challenges orthopedic implant design. Conventional 3D‐printed Ti6Al4V scaffolds are mechanically robust but suffer from poor regeneration in osteoporotic patients due to stress shielding cellular senescence. In this study, a functionalized “Cell Climbing Frame” is developed, aiming adapt the mechanical microenvironment of osteoporosis, effectively recruit support adhesion growth autologous marrow mesenchymal stem cells (BMSCs), while rejuvenating senescent for improved regeneration. Inspired marine sponges, processing accuracy limitations selective laser melting (SLM) technology broke through innovatively constructing hierarchical porous structure with macropores micropores nested within each other. Results demonstrate that unique scaffold reduces elastic modulus, facilitates blood penetration, enhances cell growth. Further surface functionalization E7 peptides exosomes promotes attraction rejuvenation BMSCs boosts migration, proliferation, osteogenic differentiation vitro. vivo, accelerates repair rats, delaying surrounding loss, enabling multi‐stage osseointegration. This innovation advances regenerative implants repair.
Язык: Английский
Процитировано
1
Biomaterials Research, Год журнала: 2025, Номер 29
Опубликована: Янв. 1, 2025
Senescent chondrocytes, which are increased in osteoarthritic (OA) cartilage, promote cartilage defects and the senescent knee microenvironment by inducing senescence to surrounding normal chondrocytes secreting senescence-associated secretory proteins. Many studies have used mesenchymal stem cells (MSCs) treat OA, but MSC treatment remains challenging for clinical application owing quality control, engraftment, fibrocartilage regeneration. Here, rather than relying on direct regeneration of MSCs, we present a novel strategy suppress OA MSC-mediated chondrocyte targeting via paracrine activity thereby improving microenvironment. First, enable control umbilical cord priming MSCs supplementing human platelet lysate (hPL) greatly enhanced functions increasing cellular glutathione levels throughout serial passaging. Intra-articular injection primed successfully suppressed progression accumulation without Indirect coculture with using transwell ameliorated phenotypes suggesting rejuvenation. Based secretome analysis, identified insulin-like growth factor 2 (IGF2) as key component that induces rejuvenation MSCs. The effects IGF2 act through autophagy activation up-regulation autophagy-related gene expression autophagic flux. To cross-validate secreted vivo, knockdown substantially abolished its therapeutic efficacy rabbit model. Collectively, these findings demonstrate hPL supplementation enables levels. mechanism was IGF2, activating autophagy.
Язык: Английский
Процитировано
0
Life Sciences, Год журнала: 2025, Номер 366-367, С. 123482 - 123482
Опубликована: Фев. 19, 2025
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2024, Номер 283, С. 137393 - 137393
Опубликована: Ноя. 7, 2024
Язык: Английский
Процитировано
1
Advanced Functional Materials, Год журнала: 2024, Номер unknown
Опубликована: Дек. 11, 2024
Abstract With the improvement of implant design and expansion application scenarios, orthopedic implants have become a common surgical option for treating fractures end‐stage osteoarthritis. Their goal is rapidly forming long‐term stable osseointegration. However, this fixation effect limited by surface characteristics peri‐implant bone tissue activity. Therefore, review summarizes strategies interface engineering (osteogenic peptides, growth factors, metal ions) treatment methods (porous nanotubes, hydrogel embedding, other load‐release systems) through research on its biological mechanism, paving way to achieve adaptation both coordination between different strategies. transition osseointegration stage, demonstrated varying therapeutic effects. Especially, activity osteoblasts runs almost entire process osseointegration, their physiological activities play dominant role in formation. Furthermore, diseases impacting metabolism exacerbate difficulty achieving This aims assist future improve implant‐bone fixation, promote fracture healing, enhance post‐implantation recovery.
Язык: Английский
Процитировано
1
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Янв. 5, 2024
Abstract Bone marrow stromal/stem cells (BMSCs) are primitive and heterogeneous that can be differentiated into osteoblasts, adipocytes other subsets. Their bone-fat lineage commitment is responsible for the homeostasis of bone microenvironment. However, there little effective methods evidence to simultaneously evaluate BMSCs. Here we provide a mixed osteo-adipogenic (OA) differentiation medium could enable BMSCs osteoblasts in vitro, establish method simultaneous assessment allocation based on Alizarin red S Oil O staining, which have been used detection specific mineralized nodules lipid droplets, respectively. This assay provides specifically simple but low-cost visualize osteogenesis adipogenesis BMSCs, efficiency
Язык: Английский
Процитировано
0
Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 734, С. 150786 - 150786
Опубликована: Окт. 2, 2024
Язык: Английский
Процитировано
0
Bone, Год журнала: 2024, Номер unknown, С. 117382 - 117382
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
0