Nanomedicine,
Год журнала:
2025,
Номер
unknown, С. 1 - 18
Опубликована: Янв. 31, 2025
Advancements
in
tissue
regeneration,
particularly
bone
regeneration
is
key
area
of
research
due
to
potential
novel
therapeutic
approaches.
Efforts
reduce
reliance
on
autologous
and
allogeneic
grafts
have
led
the
development
biomaterials
that
promote
synchronized
controlled
healing.
However,
use
growth
factors
limited
by
their
short
half-life,
slow
penetration,
large
molecular
size
toxicity.
These
suggest
traditional
delivery
methods
may
be
inadequate
hence,
address
these
challenges,
new
strategies
are
being
explored.
approaches
include
bioactive
substances
within
advanced
systems
enable
precise
spatiotemporal
control.
Dual-release
composite
scaffolds
offer
a
promising
solution
reducing
need
for
multiple
surgical
interventions
simplifying
treatment
process.
allow
sustained
drug
release,
enhancing
repair
while
minimizing
drawbacks
conventional
methods.
This
review
explores
various
dual-drug
release
systems,
discussing
modes
action,
types
drugs
used
mechanisms
improve
regeneration.
Pharmaceutics,
Год журнала:
2025,
Номер
17(4), С. 453 - 453
Опубликована: Апрель 1, 2025
Background/Objectives:
This
study
aimed
to
explore
the
therapeutic
potential
of
umbilical
mesenchymal
stem
cell-derived
apoptotic
vesicles
(UMSC-apoVs)
in
a
5-Fluorouracil
(5-FU)-induced
impairment
skin
wound
healing.
Methods:
UMSC-apoVs
were
isolated
from
UMSCs
using
differential
centrifugation
after
induction
apoptosis.
A
murine
model
was
established
by
administering
5-FU
via
intravenous
tail
injection,
followed
full-thickness
creation.
Mice
received
local
injections
at
lesion
site.
Wound
healing
evaluated
based
on
closure
rates,
histological
analysis,
and
vivo/in
vitro
functional
assays.
Rotenone
(Rot)-pretreated
used
role
mitochondrial
transfer
between
cells
(SMSCs)
Results:
significantly
accelerated
5-FU-treated
mice,
as
demonstrated
enhanced
rates
findings
reduced
inflammatory
infiltration
increased
collagen
deposition.
transferred
mitochondria
SMSCs,
enhancing
viability,
proliferation,
migration
while
reducing
reactive
oxygen
species
(ROS)
production
SMSCs.
Rot
pretreatment
inhibited
effects
inducing
dysfunction
UMSC-apoVs.
Conclusions:
Our
indicate
that
improve
5-FU-induced
impaired
facilitating
transfer,
suggesting
novel
strategy
for
alleviating
chemotherapy-induced
Journal of Orthopaedic Surgery and Research,
Год журнала:
2025,
Номер
20(1)
Опубликована: Май 17, 2025
Mesenchymal
stem
cells
(MSCs),
possessing
multilineage
potential,
are
capable
of
differentiating
into
osteoblasts
and
thus
serve
as
suitable
seed
for
bone
regeneration.
Tumor
necrosis
factor
receptor
superfamily
member
11B
(TNFRSF11B)
gene
encodes
osteoprotegerin
(OPG),
which
has
a
critical
role
in
repressing
osteoclast
differentiation
been
reported
to
influence
the
adipogenic
marrow
mesenchymal
(BMMSCs).
Nevertheless,
impact
TNFRSF11B
on
osteogenic
umbilical
cord
(UCMSCs)
remains
unclear.
This
study
aimed
investigate
osteogenesis
UCMSCs
remodeling.
Differentially
expressed
genes
(DEGs)
were
identified
from
GEO
database
using
R
software.
was
transduced
by
lentiviral
vector.
Cell
capacity
assessed
ALP
staining,
TRAP
qRT-PCR
assay.
Proteomic
analysis
performed
key
proteins
TNFRSF11B-OE-UCMSCs
that
inhibit
differentiation.
We
found
upregulated
during
downregulated
UCMSCs.
overexpressing
successfully
generated
via
lentivirus
transfection.
However,
neither
overexpression
nor
treatment
with
exogenous
OPG
protein
sufficient
enhance
potential
vitro.
Conditioned
medium
TNFRSF11B-overexpressing
significantly
suppressed
RANKL-induced
differentiation,
while
no
significant
effect
observed
osteoblast
compared
control
group.
Proteome
revealed
TNFRSF11B-OE-CM
group,
expression
C1R,
MDH1,
ACLY
downregulated,
FETUB
METRNL
associated
inhibition
demonstrates
although
does
not
promote
UCMSCs,
it
may
participate
regulating
remodeling
inhibiting
