International Immunopharmacology, Год журнала: 2024, Номер 146, С. 113802 - 113802
Опубликована: Дек. 18, 2024
Язык: Английский
Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 13
Опубликована: Янв. 24, 2025
Exosomes, as key mediators of intercellular communication, have been increasingly recognized for their role in the oncogenic processes, particularly facilitating drug resistance. This article delves into emerging evidence linking exosomal lncRNAs to modulation resistance mechanisms cancers such ovarian, cervical, and endometrial cancer. It synthesizes current research findings on how these influence cancer cell survival, tumor microenvironment, chemotherapy efficacy. Additionally, review highlights potential therapeutic strategies targeting lncRNAs, proposing a new frontier overcoming By mapping interface resistance, this aims provide comprehensive understanding that could pave way innovative treatments improved patient outcomes female reproductive system cancers.
Язык: Английский
Процитировано
1
MedComm, Год журнала: 2025, Номер 6(1)
Опубликована: Янв. 1, 2025
Abstract N4‐acetylcytidine (ac4C) modification is a crucial RNA widely present in eukaryotic RNA. Previous studies have demonstrated that ac4C plays pivotal role viral infections. Despite numerous highlighting the strong correlation between and cancer progression, its detailed roles molecular mechanisms normal physiological processes progression remain incompletely understood. This review first outlines key regulatory enzyme mediating modification, N‐acetyltransferase 10 (NAT10), including critical regulating stability, transcriptional efficiency, translational fidelity. Additionally, it systematically summarizes essential functions of biological processes, stem cell fate determination, spermatogenesis oogenesis, embryonic development, cellular senescence, bone remodeling. Furthermore, this delves into central malignant proliferation, cycle arrest, EMT, drug resistance, death, metabolism, tumor immunotherapy. It also emphasizes potential NAT10 as prognostic biomarker therapeutic target for disease treatment. In summary, clarifies multifaceted both health explores NAT10‐targeted therapies with aim advancing research improving patient outcomes.
Язык: Английский
Процитировано
0
Cureus, Год журнала: 2025, Номер unknown
Опубликована: Янв. 16, 2025
Cancer is a significant global health problem characterized by increased incidence and large disparities in outcomes. There compelling need to identify novel biomarkers enhance early detection, prognosis, tailored therapies. Growth arrest-specific transcript 5 (GAS5) long noncoding RNA (lncRNA) with potential as tumor suppressor subset of cancers. Its roles the diagnosis, therapy across cancer types remain underexplored. In this study, pan-cancer comprehensive analysis expression status GAS5 was conducted using various bioinformatics tools genomic datasets, including Tumor Immune Estimation Resource (TIMER), Gene Expression Profiling Interactive Analysis (GEPIA), University Alabama at Birmingham Data Portal (UALCAN), Omnibus (GEO). The different cancers determined then evaluated for its correlation clinicopathological parameters, immune cell infiltration, survival outcome, methylation promoter. Additionally, Kaplan-Meier plotter (KMP) used overall assessment, cBioPortal tool applied genetic alteration radiation response assessment. Functional analyses were performed data from Long Noncoding Arrays (lnCAR) database, which included coexpression networks, competing endogenous (ceRNA) interactions, pathway enrichment. Our analysis, based on three showed that significantly upregulated mainly cholangiocarcinoma (CHOL), kidney renal clear carcinoma (KIRC), liver hepatocellular (LIHC) (p < 0.05). On contrary, it downregulated breast invasive (BRCA), chromophobe (KICH), uterine corpus endometrial (UCEC). High associated poor LIHC KIRC Promoter hypomethylation identified key regulatory mechanism CHOL, KIRC, exhibited positive correlations infiltration (e.g., CD4+ T cells, CD8+ macrophages) negative B dendritic cells) KIRC. network has established ceRNA interacts 36 miRNAs 95 protein-coding genes, affecting pathways like metabolism, mitogen-activated protein kinase (MAPK) signaling, cytokine-cytokine receptor interactions alterations may have an impact levels how functions during treatment. Furthermore, epigenetic control DNA suggests possible targets personalized treatment methods. This offers foundation diagnostic prognostic biomarker, especially prominent LIHC, additional relevance Identification tissue-specific mechanisms cells opens new perspectives into context-dependent functionality GAS5. These findings highlight developing targeted therapies advancing medicine, paving way future research GAS5-based strategies cancer.
Язык: Английский
Процитировано
0
BMC Cancer, Год журнала: 2025, Номер 25(1)
Опубликована: Фев. 7, 2025
Pleural Mesothelioma (PM) is a highly aggressive cancer, for which effective early detection remains challenge due to limited screening options and low sensitivity of biomarkers discovered so far. While extracellular vesicles (EVs) have emerged as promising candidates blood-based biomarkers, their role in PM has not been studied yet. In this study, we characterized the transcriptomic profile EVs secreted by primary cells explored potential biomarker source detection. We collected cell culture supernatant from early-passage cultures derived pleural effusion 4 patients. were isolated using Qiagen exoEasy Maxi kit. RNA isolation was done mirVana PARIS Finally, single-end sequencing with Illumina Novaseq 6000. identified range species expressed cells, including protein-coding (80%), long non-coding (13%), pseudogenes (4.5%), short (1.6%). detected subset genes associated previously epithelioid (32 genes) sarcomatoid molecular components (36 PM-EVs. To investigate whether these markers could serve blood, compared content PM-EVs cargo plasma healthy donors (publicly available data). Majority upregulated RNAs. Interestingly, 25 them marker genes. functional analysis revealed that PM-EV Epithelial-Mesenchymal transition, glycolysis, hypoxia. This first study characterize cultures, demonstrating Further investigation will provide new insights into disease biology therapeutic avenues.
Язык: Английский
Процитировано
0
Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(7)
Опубликована: Фев. 13, 2025
The introns of the gene encoding long noncoding RNA (lncRNA) GAS5 host up to 10 C/D box small nucleolar RNAs (snoRNAs). However, whether there is a regulatory and functional relationship between these snoRNAs unknown. Here, we show that expression SNORD80, but not other snoRNAs, parallels regulated alongside in response cellular stress. 2′-O-methylation at A496 site, located within segment complementing conserved RNA-binding region on promotes stability consequent upregulation. This methylation requires as it diminished by knockdown SNORD80 increased overexpression, similar effects manipulating fibrillarin, methyltransferase ribonucleoprotein particle (snoRNP). upregulation stress due an enhancement its stability, which associated with increase interaction fibrillarin. Collectively, results identify role for guiding stabilize GAS5. uncovers feedforward loop locus sheds light posttranscriptional mechanisms governing lncRNA expression.
Язык: Английский
Процитировано
0
BMC Pulmonary Medicine, Год журнала: 2025, Номер 25(1)
Опубликована: Фев. 13, 2025
Язык: Английский
Процитировано
0
Open Life Sciences, Год журнала: 2025, Номер 20(1)
Опубликована: Янв. 1, 2025
Radiotherapy (RT) resistance in non-small cell lung cancer (NSCLC) is a significant contributor to tumor recurrence. NAT10, an enzyme that catalyzes ac4C RNA modification, has unclear role RT resistance. This study aimed explore the function of NAT10 NSCLC. RT-resistant NSCLC lines (PC9R and A549R) were established through repeated irradiation. The impact on cellular immunity was evaluated by measuring immune populations, cytotoxicity levels, markers dysfunction. Results demonstrated elevated levels cells. Knockdown suppressed proliferation enhanced PC9R A549R cells upregulating TNF-α IFN-γ while downregulating PD-1 TIM-3. Mechanistically, mediated NAT10-dependent modification KPNB1. Furthermore, KPNB1 facilitated PD-L1 nuclear translocation, promoting escape Overexpression but impaired In conclusion, this demonstrates upregulates expression thereby via translocation. These findings reveal novel mechanism underlying
Язык: Английский
Процитировано
0
Human Immunology, Год журнала: 2024, Номер 85(5), С. 111087 - 111087
Опубликована: Авг. 16, 2024
Язык: Английский
Процитировано
0
International Immunopharmacology, Год журнала: 2024, Номер 146, С. 113802 - 113802
Опубликована: Дек. 18, 2024
Язык: Английский
Процитировано
0