Aquaculture Reports, Год журнала: 2024, Номер 40, С. 102553 - 102553
Опубликована: Дек. 7, 2024
Язык: Английский
International Journal of Biological Macromolecules, Год журнала: 2025, Номер 296, С. 139796 - 139796
Опубликована: Янв. 11, 2025
Язык: Английский
Процитировано
0
Indian Journal of Animal Research, Год журнала: 2025, Номер Of
Опубликована: Янв. 13, 2025
Apoptosis is an integral part of sperm development, quality control and differentiation. Caspases are proteolytic enzymes that play a key role in apoptosis. The aim this review article to analyze the caspases 3, 8 9 male infertility cryopreservation. also discusses effect cryopreservation on activation sperm, leading apoptosis decreased quality. Finally, explores use caspase inhibitors as strategy improve protocols preserve Caspase 3 dominant effector caspase, while involved extrinsic intrinsic apoptotic pathways, respectively. Increased activity these has been associated with various issues, such teratozoospermia, asthenozoospermia, oligozoospermia reduced cryostability. emphasizes importance understanding their potential diagnostic markers therapeutic targets for
Язык: Английский
Процитировано
0
Veterinary Research Communications, Год журнала: 2025, Номер 49(2)
Опубликована: Янв. 22, 2025
Язык: Английский
Процитировано
0
Animal Reproduction Science, Год журнала: 2025, Номер 274, С. 107792 - 107792
Опубликована: Фев. 4, 2025
Язык: Английский
Процитировано
0
London journal of interdisciplinary sciences., Год журнала: 2025, Номер 4, С. 52 - 64
Опубликована: Фев. 9, 2025
Nanotechnology is a concept much older and more prevalent than you may think.[19] This article will delve into the applications of nanotechnology in various fields medicine. Using ideas research, old new, we explore how saves, improves, and, some cases, enables life.[15] model led us to create paper covering diverse array medicinal which has most opportunity effect. Frankly, chose have nothing common other significant interesting nanotechnology. However, even with our fields, only cover fraction nanotechnology’s massive impact across practice altogether. broken almost every major sector medicine, finding use from routine practices, such as drug delivery, all way extraordinary procedures, bone regeneration. We cardiovascular, reproductive, antiviral, skeletal, surgical fields. Our studies show that no longer limited science fiction grow time technology.[19] Doctors scientists are making full capabilities by using it any cases require precision effectiveness either impossible or extremely difficult dangerous when performed human hands. makes many treatments less hazardous effective, saving improving an exponential number lives goes on. [15]
Язык: Английский
Процитировано
0
Brazilian Journal of Biology, Год журнала: 2025, Номер 85
Опубликована: Янв. 1, 2025
Abstract Infertility is a global health issue and closely related to oxidative stress, which occurs when high concentrations of free radicals surpass the protective effects antioxidant molecules enzymes. Such imbalance causes damage DNA, as well cellular proteins lipids, ultimately leading destruction blood-testis barrier. This, in turn, hinders spermatogenesis. Various plants compounds have been employed an attempt reverse these damages, such phenolic compounds. Therefore, this review aims identify main phytochemical their respective used treatment male infertility. Related information concerning was gathered from studies selected PubMed, Scopus, Web Science databases. The search conducted using combination six terms: “phenolic compounds”, “male infertility”, “testis”, “spermatozoa”, “testosterone” fertility”. These can raise testosterone levels, reduce lipid peroxidation, improve tubular histoarchitecture cases subfertility associated with diabetes mellitus. They also mitigate caused by obesity increasing serum testosterone, activity, sperm motility. When it comes fertility problems inorganic organic pollutants, effectively restore structure seminiferous tubules, increase quality. Furthermore, shown beneficial countering adverse impacts certain drugs on testicular physiology reducing apoptosis tissue, number Leydig cells, promoting spermatocyte production. However, while may cryopreservation for vitro fertilization, caution needed dosages cause irreversible Overall, plant extracts containing hold promise therapeutic avenue treating infertility metabolic disorders environmental pollutants.
Язык: Английский
Процитировано
0
Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Год журнала: 2025, Номер 16(1)
Опубликована: Фев. 18, 2025
Abstract Background Freezing-induced sperm damage, often associated with oxidative stress, can result in regulated cell death. Given that stress trigger various forms of death, the prevailing form during cryopreservation remains unknown. Our study aimed to investigate this issue using cashmere goats as a model. Results We found significant increase lyso-phospholipids frozen-thawed suggested ferroptosis. Assessment cryopreserved sperm, or without prior treatment ferroptosis apoptosis inhibitors, demonstrated efficacy inhibitors reducing freezing damage. This implicates primary death induced cryopreservation. Additionally, positive rate transferrin receptor protein 1 was significantly lower fresh live (47.8%) than thawed (71.5%), and latter dead (82.5%). By contrast, cleaved caspase-3 positivity showed no difference between but notably sperm. Conclusions findings establish dominant goat cryopreservation, providing novel insights into freezing-induced damage mechanisms. These have implications for optimizing protocols enhancing viability after freezing-thawing. Graphical
Язык: Английский
Процитировано
0
Animals, Год журнала: 2025, Номер 15(4), С. 599 - 599
Опубликована: Фев. 19, 2025
Egg yolk, commonly employed as a cryoprotectant in semen cryopreservation, contains large particle matter that can diminish quality post thaw and complicate its assessment. For this reason, we designed centrifugal treatment of chicken egg yolk to evaluate effect on the cryopreservation porcine semen. The control group (CG) was prepared with dilution by conventional mixing treatment, experimental (EG) used centrifugally treated for ultra-low-temperature freezing process carried out methods. spermatozoa were subsequently assessed various parameters, including motility, acrosome integrity rate, plasma membrane antioxidant indexes, apoptosis expression apoptosis-related genes. results showed that, freeze-thawing, viability, VSL, VCL EG significantly higher than those observed CG (p < 0.05). Additionally, enhanced compared Furthermore, exhibited lower MDA content sperm rate 0.05), while demonstrating T-AOC CAT levels 0.05) relative CG. In comparison CG, significant reduction gene TNF-a Bax whereas Bcl-2 elevated conclusion, solution formulated through processing demonstrated efficacy enhancing following subsequent thawing.
Язык: Английский
Процитировано
0
Italian Journal of Animal Science, Год журнала: 2025, Номер 24(1), С. 754 - 762
Опубликована: Фев. 25, 2025
Язык: Английский
Процитировано
0
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Март 7, 2025
Cryopreservation techniques have been widely used, especially in biomedical applications and preservation of germplasm resources. Ideally, biological materials would maintain functional integrity as well a normal structure can be recovered when needed. However, this tool does not work all the time. Ice formation growth are key challenges. The other major reason is that cryoprotective agents (CPAs) currently used do meet these needs always accompanied by their cytotoxicity. A comprehensive synergistic approach focuses on overall frozen system crucial for evolution cryopreservation methods. In review, we first summarize fundamental damage mechanisms during cryopreservation, common cryoprotectants limitations. Next, discuss interact with ice to improve outcomes. We evaluated natural synthetic materials, including sugars polymers, AFPs mimics, nucleators, hydrogels. addition, biochemical regulation, which enhances tolerance biosamples cryopreservation-induced stresses, was also mentioned. Nanotechnology, cell encapsulation, cryomesh, isochoric freezing, such scalable approaches, further discussed cryopreservation. Finally, future research directions field efficient proposed. emphasized need multidisciplinary progress address combination cryobiology technologies, biology, nanotechnology, microfluidics, 3D bioprinting, highlighted.
Язык: Английский
Процитировано
0