Inhibiting ferroptosis mitigates sheep sperm freezing damage

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 7, 2025

To evaluate the roles of apoptosis and ferroptosis in cryopreservation-induced damage to sheep sperm, with a focus on assessing effectiveness inhibitors targeting these pathways. Initial analysis compared expression apoptotic marker Cleaved-caspase3 (CL-caspase3) ferroptotic Transferrin receptor (TFRC) between fresh cryopreserved sperm. Elevated CL-caspase3 sustained high TFRC post-cryopreservation suggested concurrent occurrence ferroptosis. Consequently, study employed Deferoxamine Mesylate (DFO), ferrostatin-1 (Fer-1), liproxstatin-1 (Lip-1), inhibitor Z-VAD-FMK (Z-VAD) at concentrations ranging from 0 10 μM. Post-thaw assessments encompassed plasma membrane integrity, acrosome biomarkers. Additional experiments were conducted measure GPX4, key regulator Optimal (2 μM for DFO, Fer-1, Lip-1; 5 Z-VAD) significantly improved sperm motility integrity. Among these, Fer-1 demonstrated greatest efficacy, reducing reactive oxygen species (ROS), lipid peroxidation, Fe2+ levels. Z-VAD primarily decreased ROS but was less potent than inhibitors. Notably, Glutathione Peroxidase 4 (GPX4) reduced post-cryopreservation, while supplementation restored its levels those comparable Both play critical cryopreservation. effectively enhanced cryopreservation outcomes by inhibiting ferroptosis, as evidenced restoration GPX4 improvement quality indicators. These findings highlight inhibition promising strategy preserving genetic material, implications animal breeding biodiversity conservation.

Language: Английский

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Role of Caspase 3, Caspase 8 and Caspase 9 in Male Infertility and Sperm Cryoinjury: A Review

Indian Journal of Animal Research, Journal Year: 2025, Volume and Issue: Of

Published: Jan. 13, 2025

Apoptosis is an integral part of sperm development, quality control and differentiation. Caspases are proteolytic enzymes that play a key role in apoptosis. The aim this review article to analyze the caspases 3, 8 9 male infertility cryopreservation. also discusses effect cryopreservation on activation sperm, leading apoptosis decreased quality. Finally, explores use caspase inhibitors as strategy improve protocols preserve Caspase 3 dominant effector caspase, while involved extrinsic intrinsic apoptotic pathways, respectively. Increased activity these has been associated with various issues, such teratozoospermia, asthenozoospermia, oligozoospermia reduced cryostability. emphasizes importance understanding their potential diagnostic markers therapeutic targets for

Language: Английский

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Optimization of canine sperm cryopreservation by focusing on glycerol concentration and freezing rate

Veterinary Research Communications, Journal Year: 2025, Volume and Issue: 49(2)

Published: Jan. 22, 2025

Language: Английский

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Protective effect of supplementation with water soluble β-carotene and α-tocopherol in boar sperm cooling-freezing extender, but not in the thawing extender

Animal Reproduction Science, Journal Year: 2025, Volume and Issue: 274, P. 107792 - 107792

Published: Feb. 4, 2025

Language: Английский

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Nanotechnological Innovations in Healthcare

London journal of interdisciplinary sciences., Journal Year: 2025, Volume and Issue: 4, P. 52 - 64

Published: Feb. 9, 2025

Nanotechnology is a concept much older and more prevalent than you may think.[19] This article will delve into the applications of nanotechnology in various fields medicine. Using ideas research, old new, we explore how saves, improves, and, some cases, enables life.[15] model led us to create paper covering diverse array medicinal which has most opportunity effect. Frankly, chose have nothing common other significant interesting nanotechnology. However, even with our fields, only cover fraction nanotechnology’s massive impact across practice altogether. broken almost every major sector medicine, finding use from routine practices, such as drug delivery, all way extraordinary procedures, bone regeneration. We cardiovascular, reproductive, antiviral, skeletal, surgical fields. Our studies show that no longer limited science fiction grow time technology.[19] Doctors scientists are making full capabilities by using it any cases require precision effectiveness either impossible or extremely difficult dangerous when performed human hands. makes many treatments less hazardous effective, saving improving an exponential number lives goes on. [15]

Language: Английский

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Protective effects of phenolic phytochemicals on male fertility: a narrative review

Brazilian Journal of Biology, Journal Year: 2025, Volume and Issue: 85

Published: Jan. 1, 2025

Abstract Infertility is a global health issue and closely related to oxidative stress, which occurs when high concentrations of free radicals surpass the protective effects antioxidant molecules enzymes. Such imbalance causes damage DNA, as well cellular proteins lipids, ultimately leading destruction blood-testis barrier. This, in turn, hinders spermatogenesis. Various plants compounds have been employed an attempt reverse these damages, such phenolic compounds. Therefore, this review aims identify main phytochemical their respective used treatment male infertility. Related information concerning was gathered from studies selected PubMed, Scopus, Web Science databases. The search conducted using combination six terms: “phenolic compounds”, “male infertility”, “testis”, “spermatozoa”, “testosterone” fertility”. These can raise testosterone levels, reduce lipid peroxidation, improve tubular histoarchitecture cases subfertility associated with diabetes mellitus. They also mitigate caused by obesity increasing serum testosterone, activity, sperm motility. When it comes fertility problems inorganic organic pollutants, effectively restore structure seminiferous tubules, increase quality. Furthermore, shown beneficial countering adverse impacts certain drugs on testicular physiology reducing apoptosis tissue, number Leydig cells, promoting spermatocyte production. However, while may cryopreservation for vitro fertilization, caution needed dosages cause irreversible Overall, plant extracts containing hold promise therapeutic avenue treating infertility metabolic disorders environmental pollutants.

Language: Английский

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Ferroptosis emerges as the predominant form of regulated cell death in goat sperm cryopreservation

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 18, 2025

Abstract Background Freezing-induced sperm damage, often associated with oxidative stress, can result in regulated cell death. Given that stress trigger various forms of death, the prevailing form during cryopreservation remains unknown. Our study aimed to investigate this issue using cashmere goats as a model. Results We found significant increase lyso-phospholipids frozen-thawed suggested ferroptosis. Assessment cryopreserved sperm, or without prior treatment ferroptosis apoptosis inhibitors, demonstrated efficacy inhibitors reducing freezing damage. This implicates primary death induced cryopreservation. Additionally, positive rate transferrin receptor protein 1 was significantly lower fresh live (47.8%) than thawed (71.5%), and latter dead (82.5%). By contrast, cleaved caspase-3 positivity showed no difference between but notably sperm. Conclusions findings establish dominant goat cryopreservation, providing novel insights into freezing-induced damage mechanisms. These have implications for optimizing protocols enhancing viability after freezing-thawing. Graphical

Language: Английский

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Effect of Centrifuged Chicken Egg Yolk on the Cryopreservation of Boar Semen

Animals, Journal Year: 2025, Volume and Issue: 15(4), P. 599 - 599

Published: Feb. 19, 2025

Egg yolk, commonly employed as a cryoprotectant in semen cryopreservation, contains large particle matter that can diminish quality post thaw and complicate its assessment. For this reason, we designed centrifugal treatment of chicken egg yolk to evaluate effect on the cryopreservation porcine semen. The control group (CG) was prepared with dilution by conventional mixing treatment, experimental (EG) used centrifugally treated for ultra-low-temperature freezing process carried out methods. spermatozoa were subsequently assessed various parameters, including motility, acrosome integrity rate, plasma membrane antioxidant indexes, apoptosis expression apoptosis-related genes. results showed that, freeze-thawing, viability, VSL, VCL EG significantly higher than those observed CG (p < 0.05). Additionally, enhanced compared Furthermore, exhibited lower MDA content sperm rate 0.05), while demonstrating T-AOC CAT levels 0.05) relative CG. In comparison CG, significant reduction gene TNF-a Bax whereas Bcl-2 elevated conclusion, solution formulated through processing demonstrated efficacy enhancing following subsequent thawing.

Language: Английский

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Effects of Different Cryoprotectants on Cryopreservation of Sperm from Noble Scallop Mimachlamys nobilis

Journal of Marine Science and Engineering, Journal Year: 2025, Volume and Issue: 13(3), P. 607 - 607

Published: March 19, 2025

In cryopreservation technology, the choice of cryoprotectant plays a crucial role in cell survival and function. Different types cryoprotectants, each with unique protective mechanisms, mitigate cellular damage from ice crystal formation during freezing. This study investigated effects different concentrations cryoprotectants on efficacy noble scallop Mimachlamys nobilis sperm. Six were tested, including four permeable (dimethyl sulfoxide (DMSO), ethylene glycerol (EG), propylene (PG), methanol (MET)) two non-permeable (trehalose (TRE), fetal bovine serum (FBS)). The results showed that which penetrate membrane, regulate osmotic pressure inside outside cells to reduce dehydration damage. Among them, 10% DMSO provided best protection, significantly preserving sperm motility, velocity, morphology. Non-permeable although unable cells, stabilized extracellular environment at higher (such as FBS). Additionally, MET FBS exhibited enhanced increasing concentration, indicating their potential reducing structural concentrations. Morphological observations indicated freezing caused varying degrees sperm, flagellar integrity being for motility. Overall, selecting an appropriate concentration is essential efficient M. providing valuable reference conserving germplasm resources marine species.

Language: Английский

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The Oxidative Stress of Human Sperm Cryopreservation

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 402 - 402

Published: March 28, 2025

Due to their negligible cytoplasm and composition of the sperm plasmalemma, spermatozoa are particularly vulnerable lipid peroxidative damage induced by reactive oxygen species (ROS). Most ROS referred as free radicals because they have unpaired electrons, causing them scavenge electrons from atoms within tissues, resulting in oxidative cellular components including cell membranes, intracellular organelles, DNA. The potential consequences stress include impaired function, DNA fragmentation, apoptosis. Understanding mechanisms that mediate during cryopreservation is key development improved freezing media formulations methodology mitigate its occurrence. Historically, elucidation those has proven largely elusive complicated positive role also play messengers redox signaling different pathways More recently, been revealed most likely suspect cryopreservation-induced damage. This narrative review was intended provide an in-depth analysis involved offer insight into possible improvements cryopreservation.

Language: Английский

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