Identification and validation of five ferroptosis-related molecular signatures in keloids based on multiple transcriptome data analysis

Frontiers in Molecular Biosciences, Год журнала: 2025, Номер 11

Опубликована: Янв. 6, 2025

Keloids are a common skin disorder characterized by excessive fibrous tissue proliferation, which can significantly impact patients' health. Ferroptosis, form of regulated cell death, plays crucial role in the development fibrosis; however, its mechanisms keloid formation remains poorly understood. This study aimed to identify key genes associated with ferroptosis formation. Data from NCBI GEO database, including GSE145725, GSE7890, and GSE44270, were analyzed, comprising total 24 17 normal samples. Additionally, single-cell data GSE181316, included 8 samples complete expression profiles, also evaluated. Differentially expressed identified, ferroptosis-related extracted GeneCards database. LASSO regression was used select keloids. Validation performed using qRT-PCR Western blot (WB) analysis on five biopsies. A 471 differentially identified GSE145725 dataset, 225 upregulated 246 downregulated genes. Five selected through gene intersection regression. Two these upregulated, while three tissue. Further GSEA pathway enrichment, GSVA set variation, immune infiltration analysis, sequencing revealed that primarily involved fibrotic process. The WB results confirmed patterns provides novel insights into molecular could serve as potential biomarkers or therapeutic targets for treating

Язык: Английский

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CAV1 promotes epithelial-to-mesenchymal transition (EMT) and chronic renal allograft interstitial fibrosis by activating the ferroptosis pathway

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Фев. 12, 2025

Background Chronic allograft dysfunction (CAD) stands as a critical factor that limits the long-term viability of transplanted kidneys. Ferroptosis is an iron-dependent form programmed cell death increasingly linked to chronic fibrosis. However, mechanism by which ferroptosis contributes onset and progression CAD remains unclear. Methods This study analyzed transcriptome data from renal transplant biopsy samples in Gene Expression Omnibus (GEO), through clinical samples, animal models, experiments, this investigated Caveolin-1 (CAV1) promotes regulation pathway. Results The elevated levels CAV1 were found positively correlate with incidence. Clinical model validation confirmed heightened expression CAD. In vitro experiments demonstrated can directly promote interstitial fibrosis regulating tubular epithelial cells; additionally, it epithelial-to-mesenchymal transition (EMT) secreting Interleukin- 6 (IL-6), thereby further contributing Conclusion plays role development promoting EMT Adjusting altering abundance may become important method for prevention treatment future.

Язык: Английский

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Noscapine Derivative 428 Suppresses Ferroptosis Through Targeting Gpx4

Опубликована: Янв. 1, 2025

Язык: Английский

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PCR array analysis reveals a novel expression profile of ferroptosis-related genes in idiopathic pulmonary fibrosis

BMC Pulmonary Medicine, Год журнала: 2025, Номер 25(1)

Опубликована: Фев. 28, 2025

Idiopathic pulmonary fibrosis (IPF) is a chronic, irreversible, and fatal disease characterized by progressive interstitial lung fibrosis. Given its insidious onset poor outcome, there an urgent need to elucidate the molecular mechanisms underlying IPF identify effective therapeutic targets diagnosis prognosis biomarkers. Ferroptosis iron-dependent form of programmed cell death that occurs as lipid peroxides accumulate. Growing evidence suggests ferroptosis important in IPF. Human PCR array was performed on control tissue. The differentially expressed ferroptosis-related genes (DE-FRGs) were identified, underwent functional enrichment analyses, protein–protein interaction network construction, potential drug target prediction. DE-FRGs validated their value diagnostic prognostic blood biomarkers evaluated using Gene Expression Omnibus dataset GSE28042. identified 13 DE-FRGs. Ontology Kyoto Encyclopedia Genes Genomes pathway analyses revealed mainly related iron ion transport, microparticles, oxidoreductase activity, involved porphyrin metabolism, necroptosis, p53 signaling addition ferroptosis. analyzed Drug–Gene Interaction Database explore novel agents, yielding 42 drugs. Four (BBC3, STEAP3, EPRS, SLC39A8) peripheral patients from GSE28042 demonstrated same expression pattern observed tissue array. receiver operating characteristic analysis area under curve STEAP3 EPRS > 0.75. survival significantly different between groups. FRG profiles characterized. findings provided valuable ideas role aided identification

Язык: Английский

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Noscapine derivative 428 suppresses ferroptosis through targeting GPX4

Redox Biology, Год журнала: 2025, Номер unknown, С. 103635 - 103635

Опубликована: Апрель 1, 2025

Язык: Английский

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Natural ingredients in the regulation of abnormal lipid peroxidation: a potential therapy for pulmonary diseases

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Дек. 20, 2024

Pulmonary diseases are a major category of that pose threat to human health. The most common drugs currently used treat lung still chemical drugs, but this may lead drug resistance and damage healthy organs in the body. Therefore, developing new is an urgent task. Lipid peroxidation caused by disruption redox homeostasis, accumulation reactive oxygen species (ROS), depletion glutathione (GSH), inactivation peroxidase 4 (GPX4). closely related occurrence progression respiratory diseases, including acute injury, asthma, pulmonary fibrosis, hypertension, chronic obstructive disease, cancer. Natural ingredients have high safety, availability, low cost, can regulate lipid through multiple pathways targets, making them valuable drugs. This article aims summarize pharmacology mechanism natural targeting treatment diseases. reviewed data indicate promising anti-lipid drug, mainly alleviating cystine/glutamate antiporter (System X

Язык: Английский

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Preparation of NIR responsive resveratrol@Au nanocages using for regulating oxidative stress microenvironment of periodontitis

Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 158774 - 158774

Опубликована: Дек. 1, 2024

Язык: Английский

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Characterization and Role of Glucagon-Like Peptide 1 Receptor in the Lacrimal Gland: Novel Insights into Diabetic Dry Eye Pathogenesis

American Journal Of Pathology, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Язык: Английский

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