Bioorganic Chemistry, Год журнала: 2024, Номер 155, С. 108113 - 108113
Опубликована: Дек. 30, 2024
Язык: Английский
npj Systems Biology and Applications, Год журнала: 2025, Номер 11(1)
Опубликована: Март 13, 2025
Cancer metabolism is characterized by significant heterogeneity, presenting challenges for treatment efficacy and patient outcomes. Understanding this heterogeneity its regulatory mechanisms at single-cell resolution crucial developing personalized therapeutic strategies. In study, we employed a network approach to characterize malignant in gynecologic breast cancers, focusing on the transcriptional driving metabolic alterations. By leveraging RNA sequencing (scRNA-seq) data, assessed pathway activities inferred cancer-specific protein-protein interactomes (PPI) gene networks (GRNs). We explored crosstalk between these identify key alterations regulation. Clustering cells pathways revealed tumor across highlighting variations oxidative phosphorylation, glycolysis, cholesterol, fatty acid, hormone, amino redox metabolism. Our analysis identified modules associated with pathways, along their regulators. These findings provide insights into complex interplay rewiring regulation paving way potential targeted strategies precision oncology. Furthermore, pipeline dissecting coregulatory can be broadly applied decipher any disease resolution.
Язык: Английский
Процитировано
0
Cellular Signalling, Год журнала: 2025, Номер unknown, С. 111753 - 111753
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Poultry Science, Год журнала: 2025, Номер unknown, С. 105059 - 105059
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Molecular Cancer, Год журнала: 2025, Номер 24(1)
Опубликована: Март 19, 2025
Abstract Extracellular vesicles (EVs) are emerging as critical mediators of intercellular communication in the tumor microenvironment (TME), profoundly influencing cancer progression. These nano-sized vesicles, released by both and stromal cells, carry a diverse cargo proteins, nucleic acids, lipids, reflecting dynamic cellular landscape mediating intricate interactions between cells. This review provides comprehensive overview biogenesis, composition, functional roles EVs cancer, highlighting their significance basic research clinical applications. We discuss how cells manipulate EV biogenesis pathways to produce enriched with pro-tumorigenic molecules, explore specific contributions key hallmarks such angiogenesis, metastasis, immune evasion, emphasizing role shaping TME driving therapeutic resistance. Concurrently, we submit recent knowledge on can serve valuable source biomarkers for minimally invasive liquid biopsies, its potential, particularly targeted drug delivery vehicles immunomodulatory agents, showcasing promise enhancing efficacy safety treatments. By deciphering messages carried EVs, gain deeper understanding biology develop more effective strategies early detection, therapy, immunotherapy, paving way new era personalized precise medicine potential significantly improve patient outcomes.
Язык: Английский
Процитировано
0
Metabolites, Год журнала: 2025, Номер 15(4), С. 221 - 221
Опубликована: Март 24, 2025
Background/Objectives: Endoplasmic reticulum (ER) stress occurs when ER homeostasis is disrupted, leading to the accumulation of misfolded or unfolded proteins. This condition activates protein response (UPR), which aims restore balance trigger cell death if cannot be achieved. In cancer, plays a key role due heightened metabolic demands tumor cells. review explores how metabolomics can provide insights into stress-related alterations and their implications for cancer therapy. Methods: A comprehensive literature was conducted analyze recent findings on stress, metabolomics, metabolism. Studies examining profiling cells under conditions were selected, with focus identifying potential biomarkers therapeutic targets. Results: Metabolomic studies highlight significant shifts in lipid metabolism, synthesis, oxidative management stress. These are crucial adaptation survival. Additionally, targeting pathways has shown preclinical models, suggesting new strategies. Conclusions: Understanding impact provides valuable opportunities drug development. Metabolomics-based approaches may help identify novel targets, enhancing effectiveness antitumor therapies.
Язык: Английский
Процитировано
0
Trends in Immunology, Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
The Journal of Steroid Biochemistry and Molecular Biology, Год журнала: 2025, Номер unknown, С. 106744 - 106744
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Cancer Biology & Therapy, Год журнала: 2025, Номер 26(1)
Опубликована: Апрель 1, 2025
Endoplasmic reticulum stress (ERS) has been implicated in the pathogenesis of various cancers, including colon cancer, by regulating tumor cell survival, growth, and immune response. However, specific genes involved ERS that could serve as prognostic markers cancer remain underexplored. This study aims to identify validate endoplasmic related (ERSRGs) correlate with patient prognosis, thereby enhancing understanding oncological outcomes potential therapeutic targeting. We utilized bioinformatics analyses ERSRGs from publicly available datasets. Differential expression analysis survival were performed assess significance these genes. Validation was conducted through quantitative real-time PCR (RT-qPCR) on selected lines. Our identified nine (ASNS, ATF4, ATF6B, BOK, CLU, DDIT3, MANF, SLC39A14, TRAF2) critical pathways IL-12, PI3K-AKT, IL-7, IL-23 signaling, linked 1-, 3-, 5-year patients cancer. A multivariate Cox model based demonstrated significant power. Further, TRAF2 strong correlated cells infiltration, suggesting its roles modulating responses microenvironment. The RT-qPCR validation confirmed differential human lines versus normal colonic epithelial line. valuable may offer new insights into targeting
Язык: Английский
Процитировано
0
Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown
Опубликована: Апрель 11, 2025
Abstract Colorectal cancer (CRC) remains a leading cause of cancer-related mortality worldwide. Natural compounds with anticancer potential, such as tomentosin, sesquiterpene lactone derived from Inula viscosa , are under investigation alternative therapeutic agents. However, its potential effects on CRC remain unexplored. This study aimed to evaluate the tomentosin in cells and elucidate underlying molecular mechanisms. HCT 116 HT- 29 were treated cell viability, colony formation, invasion, apoptosis, mitochondrial membrane (MMP), reactive oxygen species (ROS) production, autophagy, endoplasmic reticulum (ER) stress evaluated. Various assays, including XTT, Matrigel invasion used assess proliferation, invasion. Tomentosin markedly reduced viability formation dose-dependent manner. It suppressed induced evidenced by an increased apoptotic index upregulation CASP3 CASP7 CASP8 CASP9 BAX . disrupted MMP elevated ROS levels, contributing signaling. Autophagic activity was significantly upregulated, expression BECLIN1 ATG5 ATG7 MAP1LC3 A ER markers GRP78 ATF6 CHOP XBP1 also suggesting role death. has inducing modulating triggering stress. These findings underscore tomentosin’s novel candidate for CRC, warranting further vivo clinical investigations.
Язык: Английский
Процитировано
0
European Journal of Pharmacology, Год журнала: 2025, Номер unknown, С. 177623 - 177623
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0