Effects of Intercropping and Mowing Frequency on Biological Nitrogen Fixation Capacity, Nutritive Value, and Yield in Alfalfa (Medicago sativa L. cv. Vernal)

Plants, Год журнала: 2025, Номер 14(2), С. 240 - 240

Опубликована: Янв. 16, 2025

Intercropping with legume forages is recognized as an effective strategy for enhancing nitrogen levels in agroforestry, while mowing may influence fixation capacity and yield. This study investigated the rooting, fixation, nutritive value, yield of alfalfa (Medicago sativa L.) under intercropping varying frequencies (CK, 2, 3) from 2021 to 2023, using walnut (Juglans regia experimental subjects. The results indicated that suppressed root growth, whereas increased frequency stimulated development topsoil (0–20 cm). Specifically, average length density, surface area, volume twice- thrice-mowed treatments by 18.26, 17.45, 4.15%, respectively, comparison control. δ15N values intercropped were significantly lower than those monocropped (p < 0.05), mowing-thrice treatment increasing 38.61% compared but did not affect total content leaves or all resulted land equivalent ratios (LERs) greater 1. Furthermore, enhanced both value alfalfa. Our suggests can improve biological alfalfa, adopting a regime optimize value.

Язык: Английский

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The banana genome hub: a community database for genomics in the Musaceae

Horticulture Research, Год журнала: 2022, Номер 9

Опубликована: Янв. 1, 2022

The Banana Genome Hub provides centralized access for genome assemblies, annotations, and the extensive related omics resources available bananas banana relatives. A series of tools unique interfaces are implemented to harness potential genomics in bananas, leveraging power comparative analysis, while recognizing differences between datasets. Besides effective genomic like BLAST JBrowse browser, additional enable advanced gene search family analyses including multiple alignments phylogenies. synteny viewer enables comparison structures chromosome-scale assemblies. Interfaces differential expression analyses, metabolic pathways GO enrichment were also added. catalogue variants spanning diversity is made exploration, filtering, export a wide variety software. Furthermore, we new ways graphically explore presence-absence pangenomes as well ancestry mosaics cultivated bananas. Besides, guide community future sequencing efforts, provide recommendations nomenclature locus tags curated list public (assemblies, resequencing, high density genotyping) upcoming resources-planned, ongoing or not yet public. aims at supporting scientific basic, translational, applied research can be accessed https://banana-genome-hub.southgreen.fr.

Язык: Английский

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Genome-Wide Identification and Characterization of bHLH Transcription Factors Related to Anthocyanin Biosynthesis in Red Walnut (Juglans regia L.)

Frontiers in Genetics, Год журнала: 2021, Номер 12

Опубликована: Фев. 24, 2021

Basic helix-loop-helix (bHLH) proteins are transcription factors (TFs) that have been shown to regulate anthocyanin biosynthesis in many plant species. However, the bHLH gene family walnut ( Juglans regia L.) has not yet reported. In this study, 102 genes were identified genome and classified into 15 subfamilies according sequence similarity phylogenetic relationships. The structure, conserved domains, chromosome location of analyzed by bioinformatic methods. Gene duplication analyses revealed 42 JrbHLHs involved expansion family. We also characterized cis -regulatory elements these performed Ontology enrichment analysis functions, examined protein-protein interactions. Four candidate JrEGL1a , JrEGL1b JrbHLHA1 JrbHLHA2 ) found high homology encoding TFs other plants. RNA sequencing tissue- developmental stage-specific expression profiles distinct patterns phenotype (red vs. green leaves) stage red hybrid progeny, which confirmed quantitative real-time PCR analysis. All four JrbHLH localized nucleus, consistent with a TF function. These results provide basis for functional characterization investigations on molecular mechanisms walnut.

Язык: Английский

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The Manchurian Walnut Genome: Insights into Juglone and Lipid Biosynthesis

GigaScience, Год журнала: 2022, Номер 11

Опубликована: Янв. 1, 2022

Abstract Background Manchurian walnut (Juglans mandshurica Maxim.) is a tree with multiple industrial uses and medicinal properties in the Juglandaceae family (walnuts hickories). J. produces juglone, which toxic allelopathic agent has potential utilization value. Furthermore, seed of rich various unsaturated fatty acids high nutritive Findings Here, we present high-quality chromosome-scale reference genome assembly annotation for (n = 16) contig N50 21.4 Mb by combining PacBio high-fidelity reads high-throughput chromosome conformation capture data. The assembled an estimated sequence size 548.7 consists 657 contigs, 623 scaffolds, 40,453 protein-coding genes. In total, 60.99% repetitive sequences. Sixteen super-scaffolds corresponding to 16 chromosomes were assembled, scaffold length 33.7 BUSCO complete gene percentage 98.3%. displays close relationship Juglans cathayensis, divergence time 13.8 million years ago. Combining genome, transcriptome, metabolomics data, constructed gene-to-metabolite network identified 566 core conserved differentially expressed genes, may be involved juglone biosynthesis. Five CYP450 genes found that contribute accumulation. NAC, bZip, NF-YA, NF-YC are positively correlated content. Some candidate regulators (e.g., FUS3, ABI3, LEC2, WRI1 transcription factors) regulation lipid biosynthesis also identified. Conclusions Our genomic data provide new insights into evolution create platform accelerating molecular breeding improving comprehensive these economically important species.

Язык: Английский

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Genome-wide identification of KCS gene family in Carya illinoinensis and their roles under abiotic stress conditions

Scientia Horticulturae, Год журнала: 2023, Номер 321, С. 112343 - 112343

Опубликована: Июль 25, 2023

Язык: Английский

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TeaPGDB: Tea Plant Genome Database

Beverage Plant Research, Год журнала: 2021, Номер 1(1), С. 1 - 12

Опубликована: Янв. 1, 2021

As the most widely consumed beverage in world, tea has various nutritional, economic, and global cultural values. With development of third-generation sequencing technology, several genome sequences plants have been published. These genomic data pivotal information that is benefit to plant breeders biologists advancing improvement final quality products. We hereby present integrative online database, Tea Plant Genome Database (TeaPGDB; http://eplant.njau.edu.cn/tea), which incorporates published plants. The current release TeaPGDB hosts with tools, including JBrowse, gene search, SSR BLAST. also contains a download server, provides access for genome-related rich annotation files. committed collecting, integrating, annotating data, providing support research on heredity, evolution, breeding resistance, improvement, facilitating characterization important traits or flavor related genes community. Compared other databases, this database not only more complete information, but user-friendly interface researchers field.

Язык: Английский

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Genome-Wide Identification of TLP Gene Family and Their Roles in Carya cathayensis Sarg in Response to Botryosphaeria dothidea

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Апрель 1, 2022

Hickory (Carya cathayensis) is a critical tree species of the genus Carya from Juglandaceae family that contains nutrient-rich nuts. Due to large-scale soil degradation, pests and diseases hickory are becoming more serious. Thaumatin-like proteins (TLPs) vital involved in complex defense process plant pathogens. In this study, 40 CcTLP genes were identified genome-widely phylogenetically grouped into three subfamilies. The sequence CcTLPs had conservative pattern, such as eight stable disulfide bonds, REDDD, G-X-[GF]-X-C-X-T-[GA]-D-C-X(1,2)-G-X-(2,3)-C structure. total, 57 cis-elements related stress-responsive, light-responsive, phytohormone-responsive, plant-responsive discovered. Under salicylate (SA), methyl jasmonate (MeJA), ethephon (ETH) treatments, expressions CcTLP28, CcTLP29, CcTLP30, CcTLP31, CcTLP32, CcTLP33, CcTLP37, CcTLP38, CcTLP39 different patterns. This an indication most TLP upregulated by SA downregulated MeJA. Notably, seven significantly under Botryosphaeria dothidea inoculation, especially with over 20-fold change. Nine shown subcellular localization analysis be located at plasma membrane cytoplasm. knowledge disease-resistant function promoted these results. A foundation reference for molecular breeding future provided our findings.

Язык: Английский

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AlliumDB: a central portal for comparative and functional genomics in Allium

Horticulture Research, Год журнала: 2023, Номер 11(2)

Опубликована: Дек. 29, 2023

The genus

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DEFGermplasm: a comprehensive digital platform for forest genomic and phenotype data integration

Forestry Research, Год журнала: 2025, Номер 5(1), С. 0 - 0

Опубликована: Янв. 1, 2025

Язык: Английский

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Analysis of Delta(9) fatty acid desaturase gene family and their role in oleic acid accumulation in Carya cathayensis kernel

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Сен. 12, 2023

Carya cathayensis, commonly referred to as Chinese hickory, produces nuts that contain high-quality edible oils, particularly oleic acid (18:1). It is known stearoyl-ACP desaturase (SAD) the first key step converting stearic (C18:0, SA) (C18:1, OA) in aminolevulinic (ALA) biosynthetic pathway and play an important role OA accumulation. Thus far, there little information about SAD gene family C. cathayensis of individual members This study searched Hickory Genome Database identified five genes, designated CcSADs, at whole genome level through comparison with homologous genes from Arabidopsis. RNA-Seq analysis showed CcSSI2-1, CcSSI2-2, CcSAD6 were highly expressed kernels. The expression pattern CcSADs was significantly correlated fatty accumulation during kernel development. In addition, full-length cDNAs encoding SADs isolated developing cathayensis. CcSADs-green fluorescent protein (GFP) fusion construct infiltrated into tobacco epidermal cells, results indicated their chloroplast localization. catalytic function these further analyzed by heterologous Saccharomyces cerevisiae, Nicotiana benthamiana, walnut. Functional demonstrated all had activity catalyze biosynthesis. Some also have strong substrate specificity for 16:0-ACP synthesize palmitoleic (C16:1, PA). Our documented accumulation, which could be future improvement content this species via genetic manipulation.

Язык: Английский

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