Plants,
Год журнала:
2025,
Номер
14(7), С. 1142 - 1142
Опубликована: Апрель 6, 2025
Functional
U6
promoters
are
widely
utilized
in
CRISPR
gene
editing
systems
for
crops.
The
identification
of
endogenous
promoter
activity
and
the
establishment
CRISPR/Cas9
various
crops
can
enhance
efficiency
accuracy
molecular
breeding.
In
this
study,
four
snRNAs
were
identified
genome
oil
flax
(Linum
usitatissimum
L.)
cultivar
Longya
10,
which
exhibit
high
homology
with
regions
Arabidopsis
thaliana
snRNA.
We
cloned
constructed
fusion
expression
vectors
promoter-driven
dual-luciferase
reporter
genes.
Transient
transformation
Nicotiana
benthamiana
was
performed
to
measure
relative
dual
luciferase.
U6-4
on
chromosome
14
showed
highest
transcriptional
activity.
Truncations
varying
lengths
from
5'
end
tested,
revealing
that
a
342
bp
fragment
possesses
an
optimal
length.
Subsequently,
we
vector
LuU6-5P/AtU6-P
driving
LusPDS
sgRNA.
Agrobacterium-mediated
infection
hypocotyls
yielded
transgenic
albino
shoots.
DNA
these
shoots
used
as
template
amplify
fragments,
then
sequenced.
Sequencing
analysis
revealed
using
Lu14U6-4-5P
achieved
higher
frequencies
at
compared
AtU6-P-driven
systems.
Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Май 12, 2023
Plants
produce
a
wide
range
of
secondary
metabolites
that
play
vital
roles
for
their
primary
functions
such
as
growth,
defence,
adaptations
or
reproduction.
Some
the
plant
are
beneficial
to
mankind
nutraceuticals
and
pharmaceuticals.
Metabolic
pathways
regulatory
mechanism
crucial
targeting
metabolite
engineering.
The
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)/Cas9-mediated
system
has
been
widely
applied
in
genome
editing
with
high
accuracy,
efficiency,
multiplex
ability.
Besides
its
vast
application
genetic
improvement,
technique
also
facilitates
comprehensive
profiling
approach
functional
genomics
related
gene
discovery
involved
various
metabolic
pathways.
Despite
these
applications,
several
challenges
limit
CRISPR/Cas
applicability
plants.
This
review
highlights
updated
applications
system-mediated
engineering
plants
challenges.
Plants,
Год журнала:
2024,
Номер
13(14), С. 1942 - 1942
Опубликована: Июль 15, 2024
Factors
such
as
extreme
temperatures,
light
radiation,
and
nutritional
condition
influence
the
physiological,
biochemical,
molecular
processes
associated
with
fruit
development
its
quality.
Besides
abiotic
stresses,
biotic
constraints
can
also
affect
growth
Moreover,
there
be
interactions
between
stressful
conditions.
However,
it
is
challenging
to
predict
generalize
risks
of
climate
change
scenarios
on
seasonal
patterns
growth,
development,
yield,
quality
species
because
their
responses
are
often
highly
complex
involve
changes
at
multiple
levels.
Advancements
in
genetic
editing
technologies
hold
great
potential
for
agricultural
sector,
particularly
enhancing
crop
traits.
These
improvements
tailored
meet
consumer
preferences,
which
crucial
commercial
success.
Canopy
management
innovative
training
systems
key
factors
that
contribute
maximizing
yield
efficiency
improving
quality,
essential
competitiveness
orchards.
creation
habitats
support
pollinators
a
critical
aspect
sustainable
agriculture,
they
play
significant
role
production
many
crops,
including
fruits.
Incorporating
these
strategies
allows
growers
adapt
changing
conditions,
increasingly
important
stability
food
production.
By
investing
areas,
stay
ahead
challenges
opportunities
industry,
ultimately
leading
increased
success
profitability.
In
this
review,
we
aim
provide
an
updated
overview
current
knowledge
topic.
We
recommendations
future
research.
Molecular Horticulture,
Год журнала:
2025,
Номер
5(1)
Опубликована: Янв. 2, 2025
Abstract
Grapevine
is
an
important
economic
fruit
tree
worldwide,
but
grape
production
has
been
plagued
by
a
vast
number
of
fungal
diseases,
which
affect
vigor
and
the
quality
yield
berries.
To
seek
remedies
for
such
issues,
researchers
have
always
committed
to
conventional
biotechnological
breeding.
In
recent
years,
increasing
progress
made
in
elucidating
molecular
mechanisms
grape–pathogenic
fungi
interactions
resistance
regulation.
Here,
we
summarize
current
knowledge
on
basis
grapevine
including
effector-mediated
susceptibility
resistance,
resistant
regulatory
networks
grapevine,
innovative
approaches
genetic
transformation,
strategies
improve
resistance.
Understanding
exploring
accurately
regulating
diseases.
Abstract
Anthocyanins
are
plant
secondary
metabolites
that
have
a
variety
of
biological
functions,
including
pigmentation.
The
accumulation
anthocyanins
is
regulated
by
both
transcriptional
activators
and
repressors.
Studies
shown
bZIP
family
members
act
primarily
as
positive
regulators
anthocyanin
biosynthesis,
but
there
few
reports
negative
regulation.
Here,
we
report
grapevine
(Vitis
vinifera)
gene
from
group
K,
VvbZIP36,
acts
regulator
biosynthesis.
Knocking
out
one
allele
VvbZIP36
in
with
CRISPR/Cas9
promoted
accumulation.
Correlation
analysis
transcriptome
metabolome
data
showed
range
biosynthesis
genes
were
activated
mutant
plants
relative
to
the
wild
type,
resulting
related
metabolites,
naringenin
chalcone,
naringenin,
dihydroflavonols,
cyanidin-3-O-glucoside.
synthesis
stilbenes
(α-viniferin),
lignans,
some
flavonols
(including
quercetin-3-O-rhamnoside,
kaempferol-3-O-rhamnoside,
kaempferol-7-O-rhamnoside)
was
significantly
inhibited,
several
linked
their
metabolism
downregulated
plants.
In
summary,
our
results
demonstrate
plays
role
balancing
flavonols,
anthocyanins.
Frontiers in Genetics,
Год журнала:
2023,
Номер
13
Опубликована: Янв. 4, 2023
The
clustered
regularly
interspaced
short
palindrome
repeat
(CRISPR)/CRISPR-associated
protein
Cas)
system
is
a
powerful
and
highly
precise
gene-editing
tool
in
basic
applied
research
for
crop
improvement
programs.
CRISPR/Cas
being
extensively
used
plants
to
improve
yield,
quality,
nutritional
value
make
them
tolerant
environmental
stresses.
consists
of
Cas
with
DNA
endonuclease
activity
one
CRISPR
RNA
transcript
that
processed
form
or
several
guide
RNAs
direct
Cas9
the
target
sequence.
expression
levels
proteins
gRNAs
significantly
influence
editing
efficiency
CRISPR/Cas-mediated
genome
editing.
This
review
focuses
on
insights
into
Pol
III
promoters
their
types
govern
sgRNA
system.
We
discussed
structural
functional
characteristics
comparison
II
promoters.
Further,
use
synthetic
increase
targeting
overcome
structural,
functional,
expressional
limitations
has
been
discussed.
Our
reports
various
studies
illustrate
endogenous
U6/U3
improving
applicative
approach
species-specific
pol
model
crops
like
Arabidopsis
tobacco,
cereals,
legumes,
oilseed,
horticultural
crops.
further
highlight
significance
optimizing
these
promoters’
systematic
identification
validation
biotic
abiotic
stress
tolerance
through
mediated
Frontiers in Plant Science,
Год журнала:
2021,
Номер
12
Опубликована: Авг. 10, 2021
Crop
production
has
been
substantially
reduced
by
devastating
fungal
and
oomycete
pathogens,
these
pathogens
continue
to
threaten
global
food
security.
Although
chemical
cultural
controls
have
used
for
crop
protection,
involve
continuous
costs
time
fungicide
resistance
among
plant
increasingly
reported.
The
most
efficient
way
protect
crops
from
is
cultivation
of
disease-resistant
cultivars.
However,
traditional
breeding
approaches
are
laborious
intensive.
Recently,
the
CRISPR/Cas9
system
utilized
enhance
disease
different
such
as
rice,
cacao,
wheat,
tomato,
grape.
This
allows
precise
genome
editing
various
organisms
via
RNA-guided
DNA
endonuclease
activity.
Beyond
in
crops,
genomes
can
also
provide
new
strategies
management.
review
focuses
on
recent
studies
against
using
system.
For
long-term
management,
targeting
multiple
mechanisms
with
insights
gained
probing
this
will
be
powerful
approaches.
Plants,
Год журнала:
2021,
Номер
11(1), С. 51 - 51
Опубликована: Дек. 24, 2021
CRISPR/Cas-mediated
genome
editing
is
a
powerful
tool
of
plant
functional
genomics.
Hairy
root
transformation
rapid
and
convenient
approach
for
obtaining
transgenic
roots.
When
combined,
these
techniques
represent
fast
effective
means
studying
gene
function.
In
this
review,
we
outline
the
current
state
art
reached
by
combination
approaches
over
seven
years.
Additionally,
discuss
origins
different
Agrobacterium
rhizogenes
strains
that
are
widely
used
hairy
transformation;
components
CRISPR/Cas
vectors,
such
as
promoters
drive
Cas
or
gRNA
expression,
types
nuclease,
selectable
screenable
markers;
application
in
The
modification
already
known
vector
pKSE401
with
addition
rice
translational
enhancer
OsMac3
encoding
fluorescent
protein
DsRed1
also
described.
Frontiers in Plant Science,
Год журнала:
2022,
Номер
13
Опубликована: Май 12, 2022
The
clustered
regularly
interspaced
short
palindromic
repeat/CRISPR-associated
protein
9
(CRISPR/Cas9)
technology
is
an
efficient
genome
editing
tool
used
in
multiple
plant
species.
However,
it
has
not
been
applied
to
Tartary
buckwheat
(Fagopyrum
tataricum),
which
important
edible
and
medicinal
crop
rich
rutin
other
flavonoids.
FtMYB45
R2R3-type
MYB
transcription
factor
that
negatively
regulates
flavonoid
biosynthesis
buckwheat.
Here,
the
CRISPR/Cas9
system
polycistronic
tRNA-sgRNA
(PTG)/Cas9
was
employed
knock
out
gene
Two
single-guide
RNAs
(sgRNAs)
were
designed
target
second
exon
of
gene.
Twelve
transgenic
hairy
roots
obtained
using
Agrobacterium
rhizogenes-mediated
transformation.
Sequencing
data
revealed
six
lines
containing
types
mutations
at
predicted
double-stranded
break
site
generated
sgRNA1.
mutation
frequency
reached
50%.
A
liquid
chromatography
coupled
with
triple
quadrupole
mass
spectrometry
(LC-QqQ-MS)
based
metabolomic
analysis
content
rutin,
catechin,
flavonoids
increased
root
mutants
compared
transformed
empty
vector.
Thus,
CRISPR/Cas9-mediated
targeted
mutagenesis
effectively
This
finding
demonstrated
for
precise
lays
foundation
function
research
quality
improvement
