Genomic diversity landscape of the honey bee gut microbiota

Nature Communications, Год журнала: 2019, Номер 10(1)

Опубликована: Янв. 25, 2019

Abstract The structure and distribution of genomic diversity in natural microbial communities is largely unexplored. Here, we used shotgun metagenomics to assess the honey bee gut microbiota, a community consisting few bacterial phylotypes. Our results show that most phylotypes are composed sequence-discrete populations, which co-exist individual bees age-specific abundance profiles. In contrast, strains present within these populations were found segregate into bees. Consequently, despite conserved phylotype composition, each harbors distinct at functional level. While ecological differentiation seems facilitate coexistence higher taxonomic levels, our findings suggest that, level strains, priority effects during assembly result individualized profiles, social lifestyle host. study underscores need move beyond phylotype-level characterizations understand function this community, illustrates its potential for strain-level analysis.

Язык: Английский

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Gut microbiota structure differs between honeybees in winter and summer

The ISME Journal, Год журнала: 2019, Номер 14(3), С. 801 - 814

Опубликована: Дек. 13, 2019

Abstract Adult honeybees harbor a specialized gut microbiota of relatively low complexity. While seasonal differences in community composition have been reported, previous studies focused on compositional changes rather than absolute bacterial loads. Moreover, little is known about the winter bees, which live much longer bees during foraging season, and are critical for colony survival. We quantified seven core members bee single over 2 years characterized 14 colonies summer winter. Our data show that total loads substantially differ between foragers, nurses, bees. Long-lived had highest lowest α-diversity, with characteristic shift toward high levels Bartonella Commensalibacter, reduction opportunistic colonizers. Using gnotobiotic experiments, we diet major contributor to observed Overall, our study reveals remarkably different from foragers nurses. Considering importance survival, future work should focus role health disease.

Язык: Английский

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Mechanisms underlying gut microbiota–host interactions in insects

Journal of Experimental Biology, Год журнала: 2021, Номер 224(2)

Опубликована: Янв. 15, 2021

ABSTRACT Insects are the most diverse group of animals and colonize almost all environments on our planet. This diversity is reflected in structure function microbial communities inhabiting insect digestive system. As mammals, gut microbiota insects can have important symbiotic functions, complementing host nutrition, facilitating dietary breakdown or providing protection against pathogens. There an increasing number models that experimentally tractable, mechanistic studies microbiota–host interactions. In this Review, we will summarize recent findings advanced understanding molecular mechanisms underlying symbiosis between their microbiota. We open article with a general introduction to then turn towards discussion particular processes governing colonization environment as well beneficial roles mediated by The Review highlights that, although active field research implications for fundamental applied science, still early stage mechanisms. However, expanding capability culture microbiomes manipulate microbe–host interactions promises new insights from symbioses.

Язык: Английский

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Specific Strains of Honeybee Gut Lactobacillus Stimulate Host Immune System to Protect against Pathogenic Hafnia alvei

Microbiology Spectrum, Год журнала: 2022, Номер 10(1)

Опубликована: Янв. 5, 2022

Honeybee gut microbiota plays an important role in host physiology and metabolism. Recent studies have shown that the influence of resident microorganisms regulation honeybee immune system is profound, which protects against pathogen Serratia marcescens. However, only few core members functions been studied. Here, we explored how different bee bacterial species aided clearance pathogenic Hafnia alvei, causes septicemia with a high mortality rate. We found both Gilliamella apicola W8136 Lactobacillus apis W8172 protect honeybees from opportunistic pathogen, while two other strains did not affect invasion H. alvei. Transcriptomic analysis revealed induced expression profiles gut. Specifically, regulator genes Toll pathway, PGRP-S3 recognizing Gram-positive Spätzle bind to protein for downstream signal transduction, were elevated by L. apis. Correspondingly, multiple encoding antibacterial proteins also stimulated Interestingly, increased apidaecin, exhibited vitro inhibitory effect on To elucidate difference host's regulation, comparative genomic analyses indicate S-layer unique are potentially involved signaling activation production. IMPORTANCE Honeybees essential pollinators supporting global agricultural economies food supplies. decline has linked several factors, infection considered one most significant contributing factors. Although limited number pathogens identified, alvei causing adult bees. In this study, showed members, Lactobacillus, can clear invasion. Mono-colonization specific stimulate pathway AMPs. apidaecin upregulated symbionts more effective pathogen. Moreover, our suggests surface-layer driver signaling, highlighting variation regulating system.

Язык: Английский

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The honeybee microbiota and its impact on health and disease

Nature Reviews Microbiology, Год журнала: 2023, Номер 22(3), С. 122 - 137

Опубликована: Дек. 4, 2023

Язык: Английский

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The role of animal hosts in shaping gut microbiome variation

Philosophical Transactions of the Royal Society B Biological Sciences, Год журнала: 2024, Номер 379(1901)

Опубликована: Март 18, 2024

Millions of years co-evolution between animals and their associated microbial communities have shaped diversified the nature relationship. Studies continue to reveal new layers complexity in host–microbe interactions, fate which depends on a variety different factors, ranging from neutral processes environmental factors local dynamics. Research is increasingly integrating ecosystem-based approaches, metagenomics mathematical modelling disentangle individual contribution ecological microbiome evolution. Within this framework, host are known be among dominant drivers composition animal species. However, extent they shape assembly evolution remains unclear. In review, we summarize our understanding how drive these dynamics conserved vary across taxa. We conclude by outlining key avenues for research highlight need implementation modifications existing theory fully capture host-associated microbiomes. This article part theme issue ‘Sculpting microbiome: determine respond colonization’.

Язык: Английский

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The Impact of Mutualisms on Species Richness

Trends in Ecology & Evolution, Год журнала: 2019, Номер 34(8), С. 698 - 711

Опубликована: Апрель 17, 2019

Язык: Английский

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Honey bee (Apis mellifera) gut microbiota promotes host endogenous detoxification capability via regulation of P450 gene expression in the digestive tract

Microbial Biotechnology, Год журнала: 2020, Номер 13(4), С. 1201 - 1212

Опубликована: Апрель 27, 2020

Summary There is growing number of studies demonstrating a close relationship between insect gut microbiota and insecticide resistance. However, the contribution honey bee to host detoxification ability has yet be investigated. In order address this question, we compared expression cytochrome P450s (P450s) genes deficient (GD) workers conventional community (CV) mortality rates pesticide residue levels GD CV treated with thiacloprid or tau ‐fluvalinate. Our results showed that promotes P450 enzymes in midgut, rate are significantly higher than those workers. Further comparisons tetracycline‐treated untreated demonstrated antibiotic‐induced dysbiosis leads attenuated midgut. The co‐treatment antibiotics pesticides reduced survival amount residues bees. Taken together, our symbiont could contribute health through modification xenobiotics pathways revealed potential negative impact health.

Язык: Английский

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The gut microbiome defines social group membership in honey bee colonies

Science Advances, Год журнала: 2020, Номер 6(42)

Опубликована: Окт. 14, 2020

Sharing gut bacteria provides the ticket for hive membership in honey bees.

Язык: Английский

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Thiacloprid exposure perturbs the gut microbiota and reduces the survival status in honeybees

Journal of Hazardous Materials, Год журнала: 2019, Номер 389, С. 121818 - 121818

Опубликована: Дек. 3, 2019

Язык: Английский

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