Nature Reviews Microbiology, Год журнала: 2023, Номер 22(5), С. 291 - 308
Опубликована: Дек. 18, 2023
Язык: Английский
Immunity, Год журнала: 2020, Номер 52(6), С. 910 - 941
Опубликована: Май 6, 2020
Язык: Английский
Процитировано
1660
Nature Biomedical Engineering, Год журнала: 2021, Номер 5(9), С. 951 - 967
Опубликована: Апрель 1, 2021
Язык: Английский
Процитировано
971
Science, Год журнала: 2021, Номер 371(6536)
Опубликована: Март 25, 2021
Microbial roles in cancer formation, diagnosis, prognosis, and treatment have been disputed for centuries. Recent studies provocatively claimed that bacteria, viruses, and/or fungi are pervasive among cancers, key actors immunotherapy, engineerable to treat metastases. Despite these findings, the number of microbes known directly cause carcinogenesis remains small. Critically evaluating building frameworks such evidence light modern biology is an important task. In this Review, we delineate between causal complicit trace common themes their influence through host's immune system, herein defined as immuno-oncology-microbiome axis. We further review intratumoral approaches manipulate gut or tumor microbiome while projecting next phase experimental discovery.
Язык: Английский
Процитировано
878
Nature Communications, Год журнала: 2022, Номер 13(1)
Опубликована: Июнь 14, 2022
Abstract Reactive oxygen species (ROS) play vital roles in intestinal inflammation. Therefore, eliminating ROS the inflammatory site by antioxidant enzymes such as catalase and superoxide dismutase may effectively curb bowel disease (IBD). Here, Escherichia coli Nissle 1917 (ECN), a kind of oral probiotic, was genetically engineered to overexpress (ECN-pE) for treatment To improve bioavailability ECN-pE gastrointestinal tract, chitosan sodium alginate, effective biofilms, were used coat via layer-by-layer electrostatic self-assembly strategy. In mouse IBD model induced different chemical drugs, chitosan/sodium alginate coating (ECN-pE(C/A) 2 ) relieved inflammation repaired epithelial barriers colon. Unexpectedly, EcN-pE(C/A) could also regulate microbial communities abundance Lachnospiraceae _NK4A136 Odoribacter flora, which are important microbes maintain homeostasis. Thus, this study lays foundation development living therapeutic proteins using probiotics treat intestinal-related diseases.
Язык: Английский
Процитировано
371
Cancer Cell, Год журнала: 2021, Номер 39(10), С. 1317 - 1341
Опубликована: Сен. 9, 2021
Язык: Английский
Процитировано
362
Nature Reviews Drug Discovery, Год журнала: 2022, Номер 21(5), С. 339 - 358
Опубликована: Фев. 16, 2022
Язык: Английский
Процитировано
360
Nature Reviews Drug Discovery, Год журнала: 2021, Номер 20(12), С. 941 - 960
Опубликована: Окт. 6, 2021
Язык: Английский
Процитировано
217
Advanced Functional Materials, Год журнала: 2021, Номер 31(48)
Опубликована: Сен. 1, 2021
Abstract Engineering therapeutic angiogenesis in impaired tissues is critical for chronic wound healing. Materials can be engineered to deliver specific biological cues that enhance angiogenesis. However, currently available materials have limitations use engineering since the complex inflammation environment of wounds requires spatiotemporal control. Immune cells are central component microenvironment and orchestrate immune responses This study presents a novel approach using delivery system comprising living Lactococcus , incorporated heparin‐poloxamer thermoresponsive hydrogel, designed bioengineer highly dynamic‐temporal manner. The produce protect vascular endothelial growth factor (VEGF) increase proliferation, migration, tube formation cells, as well secrete lactic acid shift macrophages toward an anti‐inflammatory phenotype, resulting successful diabetic wounds. Further, confines bacterial population wounds, thereby minimizing risk systemic toxicities. Therefore, this hydrogel harnessed safe efficient therapeutics drive rapid healing may serve promising scaffold regenerative medicine.
Язык: Английский
Процитировано
184
Nature Communications, Год журнала: 2021, Номер 12(1)
Опубликована: Март 2, 2021
Abstract Synthetic biology holds great promise for addressing global needs. However, most current developments are not immediately translatable to ‘outside-the-lab’ scenarios that differ from controlled laboratory settings. Challenges include enabling long-term storage stability as well operating in resource-limited and off-the-grid using autonomous function. Here we analyze recent advances developing synthetic biological platforms outside-the-lab with a focus on three major application spaces: bioproduction, biosensing, closed-loop therapeutic probiotic delivery. Across the Perspective, highlight advances, areas further development, possibilities future applications, needs innovation at interface of other disciplines.
Язык: Английский
Процитировано
174
Nature Biotechnology, Год журнала: 2022, Номер 40(8), С. 1259 - 1269
Опубликована: Март 17, 2022
Abstract Living bacteria therapies have been proposed as an alternative approach to treating a broad array of cancers. In this study, we developed genetically encoded microbial encapsulation system with tunable and dynamic expression surface capsular polysaccharides that enhances systemic delivery. Based on small RNA screen biosynthesis pathways, constructed inducible synthetic gene circuits regulate bacterial in Escherichia coli Nissle 1917. These are capable temporarily evading immune attack, whereas subsequent loss results effective clearance vivo. This delivery strategy enabled ten-fold increase maximum tolerated dose improved anti-tumor efficacy murine models cancer. Furthermore, situ increased the fraction translocation among mouse tumors, leading distal tumors. The programmable promises enhance therapeutic utility living engineered for
Язык: Английский
Процитировано
174