Periodontology 2000,
Год журнала:
2022,
Номер
89(1), С. 166 - 180
Опубликована: Март 4, 2022
Abstract
Accumulating
evidence
demonstrates
that
the
oral
pathobiont
Fusobacterium
nucleatum
is
involved
in
progression
of
an
increasing
number
tumors
types.
Thus
far,
mechanisms
underlying
tumor
exacerbation
by
F.
include
enhancement
proliferation,
establishment
a
tumor‐promoting
immune
environment,
induction
chemoresistance,
and
activation
checkpoints.
This
review
focuses
on
mediate
tumor‐specific
colonization
fusobacteria.
Elucidating
mediating
fusobacterial
tropism
promotion
might
provide
new
insights
for
development
novel
approaches
detection
treatment.
Nature,
Год журнала:
2022,
Номер
611(7937), С. 810 - 817
Опубликована: Ноя. 16, 2022
Abstract
The
tumour-associated
microbiota
is
an
intrinsic
component
of
the
tumour
microenvironment
across
human
cancer
types
1,2
.
Intratumoral
host–microbiota
studies
have
so
far
largely
relied
on
bulk
tissue
analysis
1–3
,
which
obscures
spatial
distribution
and
localized
effect
within
tumours.
Here,
by
applying
in
situ
spatial-profiling
technologies
4
single-cell
RNA
sequencing
5
to
oral
squamous
cell
carcinoma
colorectal
cancer,
we
reveal
spatial,
cellular
molecular
host–microbe
interactions.
We
adapted
10x
Visium
transcriptomics
determine
identity
location
intratumoral
microbial
communities
patient
tissues.
Using
GeoMx
digital
profiling
6
show
that
bacterial
populate
microniches
are
less
vascularized,
highly
immuno‑suppressive
associated
with
malignant
cells
lower
levels
Ki-67
as
compared
bacteria-negative
regions.
developed
a
RNA-sequencing
method
name
INVADEseq
(invasion–adhesion-directed
expression
sequencing)
and,
this
tumours,
identify
cell-associated
bacteria
host
they
interact,
well
uncovering
alterations
transcriptional
pathways
involved
inflammation,
metastasis,
dormancy
DNA
repair.
Through
functional
studies,
infected
invade
their
surrounding
environment
single
recruit
myeloid
Collectively,
our
data
not
random;
instead,
it
organized
immune
epithelial
functions
promote
progression.
Cell,
Год журнала:
2022,
Номер
185(20), С. 3789 - 3806.e17
Опубликована: Сен. 1, 2022
Cancer-microbe
associations
have
been
explored
for
centuries,
but
cancer-associated
fungi
rarely
examined.
Here,
we
comprehensively
characterize
the
cancer
mycobiome
within
17,401
patient
tissue,
blood,
and
plasma
samples
across
35
types
in
four
independent
cohorts.
We
report
fungal
DNA
cells
at
low
abundances
many
major
human
cancers,
with
differences
community
compositions
that
differ
among
types,
even
when
accounting
technical
background.
Fungal
histological
staining
of
tissue
microarrays
supported
intratumoral
presence
frequent
spatial
association
macrophages.
Comparing
communities
matched
bacteriomes
immunomes
revealed
co-occurring
bi-domain
ecologies,
often
permissive,
rather
than
competitive,
microenvironments
distinct
immune
responses.
Clinically
focused
assessments
suggested
prognostic
diagnostic
capacities
mycobiomes,
stage
I
synergistic
predictive
performance
bacteriomes.
Science,
Год журнала:
2022,
Номер
378(6622), С. 858 - 864
Опубликована: Ноя. 24, 2022
With
increasing
evidence
that
microbes
colonize
tumors,
synthetic
biology
tools
are
being
leveraged
to
repurpose
bacteria
as
tumor-specific
delivery
systems.
These
engineered
systems
can
modulate
the
tumor
microenvironment
using
a
combination
of
their
inherent
immunogenicity
and
local
payload
production.
Here,
we
review
genetic
circuits
enhance
spatial
temporal
control
therapeutic
improve
safety
efficacy.
We
describe
engineering
interactions
among
bacteria,
cells,
immune
progression
from
single
agents
toward
rational
with
other
modalities.
Together,
these
efforts
building
an
emerging
concept
between
programmable
medicines
biology.
