Gene-edited pigs: a translational model for human food allergy against alpha-Gal and anaphylaxis DOI Creative Commons
Ying Wang, Miriam Hils, Andrea Fischer

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Фев. 26, 2024

The prevalence of food allergy is rising and estimated to approach 10%. Red meat the first known elicited by immunoglobulin E (IgE) antibodies recognizing a carbohydrate. Due loss function alpha−1,3−galactosyltransferase ( GGTA1) gene in humans, disaccharide galactose-α-1,3-galactose (α-Gal) cannot be synthesized therefore became immunogenic. IgE sensitization through skin repetitive tick bites transmitting α-Gal. underlying mechanisms regarding innate adaptive immune cell activation, including B-cell isotype switch IgE, are poorly understood, requiring further research physiologically relevant animal models. Here, we describe new model red using percutaneous α-Gal gene-edited GGTA1 -deficient pigs. Total α-Gal-specific IgG, IgG1, IgG2, IgG4, levels were tracked. Further key factors associated with allergic inflammation, type 2 immunity, development measured PBMCs samples. Significant increases IgG1 indicated successful allergen Intracutaneous sensitizations recruited lymphocytes skin, elevated numbers T helper (Th2) cells. Finally, α-Gal-sensitized pigs not only recognized as non-self-antigen following exposure but also developed anaphylaxis upon antigen challenge. Based on similarities between porcine human this large for should help unveil consecutive steps cutaneous aid prophylactic treatment interventions.

Язык: Английский

Automated analysis of genomic sequences facilitates high-throughput and comprehensive description of bacteria DOI Creative Commons
Thomas C. A. Hitch, Thomas Riedel, Aharon Oren

и другие.

ISME Communications, Год журнала: 2021, Номер 1(1)

Опубликована: Май 20, 2021

The study of microbial communities is hampered by the large fraction still unknown bacteria. However, many these species have been isolated, yet lack a validly published name or description. validation names for novel bacteria requires that uniqueness those taxa demonstrated and their properties are described. accepted format this protologue, which can be time-consuming to create. Hence, research fields in microbiology biotechnology will greatly benefit from new approaches reduce workload harmonise generation protologues.We developed Protologger, bioinformatic tool automatically generates all necessary readouts writing detailed protologue. By producing multiple taxonomic outputs, functional features ecological analysis using 16S rRNA gene genome sequences single species, time needed gather information describing substantially reduced. usefulness Protologger was three isolate collections describe 34 taxa, encompassing 17 genera, including automatic ecologically functionally relevant names. We also highlight need utilise delineation methods, as while inconsistencies between each method occur, combined approach provides robust placement. open source; scripts datasets available, along with webserver at www.protologger.de.

Язык: Английский

Процитировано

289
Valid publication of new names and new combinations effectively published outside the IJSEM DOI Open Access
Aharon Oren, George M Garrity

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Год журнала: 2021, Номер 71(7)

Опубликована: Авг. 2, 2021

Microbiology Society journals contain high-quality research papers and topical review articles. We are a not-for-profit publisher we support invest in the microbiology community, to benefit of everyone. This supports our principal goal develop, expand strengthen networks available members so that they can generate new knowledge about microbes ensure it is shared with other communities.

Язык: Английский

Процитировано

159
Dynamic Distribution of Gut Microbiota in Pigs at Different Growth Stages: Composition and Contribution DOI

Yuheng Luo,

Wen Ren, Hauke Smidt

и другие.

Microbiology Spectrum, Год журнала: 2022, Номер 10(3)

Опубликована: Май 18, 2022

The ban on feed antibiotics by more and countries, the expected ZnO in supplementation from 2022 EU, urge researchers pig producers to search for new alternatives. One possible alternative is use so-called “next-generation probiotics (NGPs)” derived gastrointestinal tract.

Язык: Английский

Процитировано

86
Exploration of the Muribaculaceae Family in the Gut Microbiota: Diversity, Metabolism, and Function DOI Open Access

Yiqing Zhu,

Borui Chen,

Xinyu Zhang

и другие.

Nutrients, Год журнала: 2024, Номер 16(16), С. 2660 - 2660

Опубликована: Авг. 12, 2024

The gut microbiota are mainly composed of

Язык: Английский

Процитировано

53
POCP-nf: an automatic Nextflow pipeline for calculating the percentage of conserved proteins in bacterial taxonomy DOI Creative Commons
Martin Hölzer

Bioinformatics, Год журнала: 2024, Номер 40(4)

Опубликована: Март 29, 2024

Abstract Summary Sequence technology advancements have led to an exponential increase in bacterial genomes, necessitating robust taxonomic classification methods. The Percentage Of Conserved Proteins (POCP), proposed initially by Qin et al. (2014), is a valuable metric for assessing prokaryote genus boundaries. Here, I introduce computational pipeline automated POCP calculation, aiming enhance reproducibility and ease of use studies. Availability implementation POCP-nf uses DIAMOND faster protein alignments, achieving similar sensitivity BLASTP. implemented Nextflow with Conda Docker support freely available on GitHub under https://github.com/hoelzer/pocp. open-source code can be easily adapted various prokaryotic genome datasets. Detailed documentation usage instructions are provided the repository.

Язык: Английский

Процитировано

16
Intestinal microbiota and its interaction to intestinal health in nursery pigs DOI Creative Commons
Marcos Elias Duarte, Sung Woo Kim

Animal nutrition, Год журнала: 2021, Номер 8, С. 169 - 184

Опубликована: Июнь 12, 2021

The intestinal microbiota has gained increased attention from researchers within the swine industry due to its role in promoting maturation, immune system modulation, and consequently enhancement of health growth performance host. This review aimed provide updated scientific information on interaction among microbiota, dietary components, pigs. small intestine is a key site evaluate diet, host because it main for digestion absorption nutrients plays an important system. diet associated components such as feed additives are factors affecting microbial composition central stimulating beneficial population microbiota. microbiota-host modulates system, and, concurrently, helps modulate composition. direct between indication that mucosa-associated can be more effective evaluating effect parameters. It was demonstrated should evaluated when analyzing diets, health. In addition, supplementation promote pigs consider their roles modulation biomarkers predict response interventions.

Язык: Английский

Процитировано

90
Early-Life Immune System Maturation in Chickens Using a Synthetic Community of Cultured Gut Bacteria DOI Creative Commons
Christian Zenner, Thomas C. A. Hitch, Thomas Riedel

и другие.

mSystems, Год журнала: 2021, Номер 6(3)

Опубликована: Май 17, 2021

The immune system plays a crucial role in sustaining animal health. Its development is markedly influenced by early microbial colonization of the gastrointestinal tract.

Язык: Английский

Процитировано

81
Gut microbiome-produced metabolites in pigs: a review on their biological functions and the influence of probiotics DOI Creative Commons
Robie Vasquez, Ju Kyoung Oh, Ji Hoon Song

и другие.

Journal of Animal Science and Technology, Год журнала: 2022, Номер 64(4), С. 671 - 695

Опубликована: Июль 1, 2022

The gastrointestinal tract is a complex ecosystem that contains large number of microorganisms with different metabolic capacities. Modulation the gut microbiome can improve growth and promote health in pigs. Crosstalk between host, diet, influence potentially through production several metabolites various functions. Short-chain branched-chain fatty acids, secondary bile polyamines, indoles, phenolic compounds are produced by microbiome. also produce neurotransmitters (such as γ-aminobutyric acid, catecholamines, serotonin), their precursors, vitamins. Several studies pigs have demonstrated importance its improving performance feed efficiency, alleviating stress, providing protection from pathogens. use probiotics one strategies employed to target Promising results been published on optimizing pig production. This review focuses role microbiome-derived effects altering levels these metabolites.

Язык: Английский

Процитировано

64
A taxonomic note on the genus Prevotella: Description of four novel genera and emended description of the genera Hallella and Xylanibacter DOI Creative Commons
Thomas C. A. Hitch, Kevin Bisdorf,

Afrizal Afrizal

и другие.

Systematic and Applied Microbiology, Год журнала: 2022, Номер 45(6), С. 126354 - 126354

Опубликована: Авг. 20, 2022

The genus Prevotella comprises 55 species with validly published, and correct, names (at June 2021) that are phenotypically, ecologically functionally diverse. This study used a range of comparative genome approaches (marker gene-based phylogeny, core average amino acid identity, percentage conserved proteins clade-specific marker genes) to identify large differences between the 53 for which genomes available, as well two effectively published yet not named four novel species. These were consistent various analysis methods justify separation into multiple genera. While distribution across 19 ecosystem types was unique each inconsistent within clades, functional repertoire based on presence/absence both PFAMs CAZy families revealed distinct clustering proposed Based integration all results, we propose reclassification previously assigned seven genera, including genera Segatella, Hoylesella, Leyella Palleniella proposed. In addition Prevotella, this work describes species, Hallella faecis, Xylanibacter rodentium, muris, intestinalis.

Язык: Английский

Процитировано

56
Genome-centric investigation of bile acid metabolizing microbiota of dairy cows and associated diet-induced functional implications DOI Creative Commons
Limei Lin, Zheng Lai,

Yang Huisheng

и другие.

The ISME Journal, Год журнала: 2022, Номер 17(1), С. 172 - 184

Опубликована: Окт. 19, 2022

Abstract Although the importance of bile acid (BA)-related microbial strains and enzymes is increasingly recognized for monogastric animals, a lack knowledge about BA metabolism in dairy cows limits functional applications aimed at targeted modulation microbe–host interactions animal production health. In present study, 108 content samples from six intestinal regions were used shotgun metagenomic sequencing. Overall, 372 high-quality metagenome-assembled genomes (MAGs) involved deconjugation, oxidation, dehydroxylation pathways. Furthermore, BA-metabolizing microbiome predominately occurred large intestine, resulting accumulation secondary unconjugated BAs. Comparative genomic analysis revealed that salt hydrolase (BSH)-carrying populations managed with selective environment cow intestine by adopting numerous host mucin glycan-degrading abilities. A sequence similarity network classified 439 BSH homologs into 12 clusters identified different diverse evolution, taxonomy, signal peptides, ecological niches. Our omics data further Firmicutes bacterium CAG-110 processed increased abundance BSHs Cluster 1, coinciding changes colon cholic concentration after grain introduction, intricately related to inflammation. This study first use genome-centric approach whole intestine-targeted metabolomics reveal its diet-induced implications cows. These findings provide insight manipulation microorganisms improving

Язык: Английский

Процитировано

50