The Sentient Cell

Oxford University Press eBooks, Год журнала: 2023, Номер unknown

Опубликована: Окт. 5, 2023

Abstract This volume is the natural follow-up to Arthur Reber’s 2019 book, The First Minds: Caterpillars, ‘Karyotes, and Consciousness (TFM). In that earlier work, Cellular Basis of (CBC) theory was developed based on a number efforts published in variety journals between 1997 as well talks, colloquia, presentations at conferences. core proposition TFM life mind are co-terminous. All organisms, all species extant extinct, sentient. have an existentially secure consciousness—without which they would been evolutionary dead-ends, unable survive chaotic, dangerous environment first appeared. And, importantly, forms sentience, cognitive functioning right up including those expressed by humans, evolved from original expression consciousness birth prokaryotes. unicellular widely accepted, foundational principle biological social sciences. CBC simply applies same sentience.

Язык: Английский

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CEP112 coordinates translational regulation of essential fertility genes during spermiogenesis through phase separation in humans and mice

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 30, 2024

Язык: Английский

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Multifaceted roles of centrosomes in development, health, and disease

Journal of Molecular Cell Biology, Год журнала: 2021, Номер 13(9), С. 611 - 621

Опубликована: Июль 12, 2021

Abstract The centrosome is a membrane-less organelle consisting of pair barrel-shaped centrioles and pericentriolar material functions as the major microtubule-organizing center signaling hub in animal cells. past decades have witnessed functional complexity importance centrosomes various cellular processes such cell shaping, division, migration. In addition, abnormalities are linked to wide range human diseases pathological states, cancer, reproductive disorder, brain disease, ciliopathies. Herein, we discuss development health, with an emphasis on their roles germ cells, stem immune responses. We also how dysfunctions involved diseases. A better understanding mechanisms regulating may lead way potential therapeutic targeting this disease treatment.

Язык: Английский

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Sperm centriole assessment identifies male factor infertility in couples with unexplained infertility – a pilot study

European Journal of Cell Biology, Год журнала: 2022, Номер 101(3), С. 151243 - 151243

Опубликована: Май 27, 2022

Unexplained infertility affects about one-third of infertile couples and is defined as the failure to identify cause despite extensive evaluation male female partners. Therefore, there a need for multiparametric approach study sperm function. Recently, we developed Fluorescence-Based Ratiometric Analysis Sperm Centrioles (FRAC) assay determine centriole quality. Here, perform pilot from 10 fertile men in with unexplained infertility, using three centriolar biomarkers measured at locations two fractions, representing high low We found that FRAC can likely source infertility. Higher quality fractions individuals were reference population. All 180 studied values fell within population range. Eleven patients outliers beyond 95% confidence intervals (P = 0.0008). Three had outlier their higher fraction, while four lower fraction (3/10 4/10, P 0.060 0.025, respectively), suggesting these are due, part, defects. propose larger scale should be performed ability factor its potential contribution analysis.

Язык: Английский

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Intracellular connections between basal bodies promote the coordinated behavior of motile cilia

Molecular Biology of the Cell, Год журнала: 2022, Номер 33(11)

Опубликована: Сен. 7, 2022

Hydrodynamic flow produced by multiciliated cells is critical for fluid circulation and cell motility. Hundreds of cilia beat with metachronal synchrony flow. Cilia-driven produces extracellular hydrodynamic forces that cause neighboring to in a synchronized manner. However, coupling between not the sole mechanism drives synchrony. Cilia are nucleated basal bodies (BBs) link each other cell's cortex via BB-associated appendages. The intracellular BB cortical network hypothesized synchronize ciliary beating transmitting coordination cues. extent connections nature these stimuli remain unclear. Moreover, how influence dynamics individual has been established. We show focused ion beam scanning electron microscopy imaging coupled both longitudinally laterally ciliate Tetrahymena thermophila underlying cytoskeletal network. To visualize behavior live, immobilized cells, we developed Delivered Iron Particle Ubiety Live Light (DIPULL) microscopy. Quantitative computer analyses reveal control waveform coordinate beating. Loss reduces cilia-dependent forces.

Язык: Английский

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The centrosome – diverse functions in fertilization and development across species

Journal of Cell Science, Год журнала: 2023, Номер 136(23)

Опубликована: Дек. 1, 2023

ABSTRACT The centrosome is a non-membrane-bound organelle that conserved across most animal cells and serves various functions throughout the cell cycle. In dividing cells, known as spindle pole nucleates robust microtubule to separate genetic material equally into two daughter cells. non-dividing mother centriole, substructure of centrosome, matures basal body cilia, which acts signal-transducing antenna. centrosomes their substructures are important for embryonic development have been studied extensively using in vitro mammalian culture or vivo invertebrate models. However, there considerable differences composition during different aspects vertebrate development, these less studied. this Review, we discuss roles played by centrosomes, highlighting divergent features species, particularly fertilization development.

Язык: Английский

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The proximal centriole-like structure maintains nucleus-centriole architecture in sperm

Journal of Cell Science, Год журнала: 2024, Номер 137(17)

Опубликована: Авг. 22, 2024

ABSTRACT Proper connection between the sperm head and tail is critical for motility fertilization. Head–tail linkage mediated by head-tail coupling apparatus (HTCA), which secures axoneme (tail) to nucleus (head). However, molecular architecture of HTCA poorly understood. Here, we use Drosophila investigate formation remodeling throughout spermiogenesis visualizing key components this complex. Using structured illumination microscopy, demonstrate that proteins Spag4 Yuri form a ‘centriole cap’ surrounds centriole (or basal body) as it invaginates into surface nucleus. As development progresses, laterally displaced side while expands under nucleus, forming what term ‘nuclear shelf’. We next show proximal centriole-like (PCL) structure positioned nuclear shelf, functioning crucial stabilizer centriole–nucleus attachment. Together, our data indicate complex, multi-point attachment site simultaneously engages PCL, ensure proper head–tail during late-stage spermiogenesis.

Язык: Английский

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How Relevant are Male Factors for Fertilization and Early Embryo Development? Looking into the (Epi)genome, Proteome and Metabolome

European Review, Год журнала: 2025, Номер unknown, С. 1 - 33

Опубликована: Янв. 31, 2025

Infertility affects 10–15% of couples at the age conception. Mounting evidence supports that not only are paternal factors crucial during fertilization, but also for embryogenesis. This review aims to provide some clues about contribution male reproductive success and live birth, as such contributions can be important female. Semen is composed two fractions: sperm seminal plasma. Regarding former, integrity components (i.e., centrioles, DNA methylation, histone-to-protamine ration, specific proteins, etc.) has been proven essential events occurring upon engulfment spermatozoon into oocyte cytoplasm. The metabolic status seems shape their potential fertilizing capacity. Furthermore, plasma appears modulate female tract, suggested support embryo implantation. In spite aforementioned, it remains largely unaddressed how interact with maternal ones, whether latter may mask former. While assisted techniques (ART) useful rescue infertility, a better understanding semen development implantation increase efficiency these techniques, address further causes total fertilization failure, deficiency recurrent miscarriage.

Язык: Английский

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Spermatozoa centriole quality determined by FRAC may correlate with zygote nucleoli polarization—a pilot study

Journal of Assisted Reproduction and Genetics, Год журнала: 2025, Номер unknown

Опубликована: Фев. 7, 2025

Spermatozoa centriolar defects can result in abnormal zygote functions. Recently, a method to quantify spermatozoa was developed named Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC). However, whether identified by FRAC functions not tested. Here, we quantified using FRAC, and centriole function assessed imaging Nucleolus Precursor Body (NPB) polarization which based on the pattern NPB polarization. Data analyzed at couple embryo levels. Subjects were divided into two groups: seven couples 62 embryos with normal centrioles versus eight 78 (140 from 15 total). Patterned statistically significant both couple- embryo-level analyses (p < 0.0001 p = 0.0024). These results suggest that may correlate centrosome via scoring. This study provides foundation for more extensive studies test FRAC's utility assessing quality.

Язык: Английский

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Superhelix flow structures drive sperm locomotion

Cell Reports Physical Science, Год журнала: 2025, Номер unknown, С. 102524 - 102524

Опубликована: Апрель 1, 2025

Язык: Английский

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