Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Янв. 3, 2023
Abstract
Spinal
motor
neurons
(MNs)
integrate
sensory
stimuli
and
brain
commands
to
generate
movements.
In
vertebrates,
the
molecular
identities
of
cardinal
MN
types
such
as
those
innervating
limb
versus
trunk
muscles
are
well
elucidated.
Yet
finer
subtypes
within
these
cell
populations
that
innervate
individual
muscle
groups
remain
enigmatic.
Here
we
investigate
heterogeneity
in
mouse
MNs
using
single-cell
transcriptomics.
Among
limb-innervating
MNs,
reveal
a
diverse
neuropeptide
code
for
delineating
putative
pool
identities.
Additionally,
uncover
axial
subdivided
into
three
molecularly
distinct
subtypes,
defined
by
mediolaterally-biased
Satb2,
Nr2f2
or
Bcl11b
expression
patterns
with
different
axon
guidance
signatures.
These
present
chicken
human
embryos,
suggesting
conserved
pattern
across
higher
vertebrates.
Overall,
our
study
provides
resource
spinal
paves
way
towards
deciphering
how
neuronal
evolved
accommodate
vertebrate
behaviors.
Cell Reports,
Год журнала:
2023,
Номер
42(9), С. 113049 - 113049
Опубликована: Сен. 1, 2023
Locomotion
requires
precise
control
of
the
strength
and
speed
muscle
contraction
is
achieved
by
recruiting
functionally
distinct
subtypes
motor
neurons
(MNs).
MNs
are
essential
to
movement
differentially
susceptible
in
disease,
but
little
known
about
how
acquire
functional
subtype-specific
features
during
development.
Using
single-cell
RNA
profiling
embryonic
larval
zebrafish,
we
identify
novel
conserved
molecular
signatures
for
MN
genes
expressed
both
early
post-mitotic
mature
MNs.
Assessing
development
genetic
mutants,
define
a
program
subtype
specification.
Two
evolutionarily
transcription
factors,
Prdm16
Mecom,
determinants
integral
fast
Loss
prdm16
or
mecom
causes
develop
transcriptional
profiles
innervation
similar
slow
These
results
reveal
diversity
vertebrate
axial
demonstrate
that
specified
through
intrinsic
codes.
Annual Review of Neuroscience,
Год журнала:
2023,
Номер
46(1), С. 79 - 99
Опубликована: Фев. 28, 2023
The
spinal
cord
is
home
to
the
intrinsic
networks
for
locomotion.
An
animal
in
which
has
been
fully
severed
from
brain
can
still
produce
rhythmic,
patterned
locomotor
movements
as
long
some
excitatory
drive
provided,
such
physical,
pharmacological,
or
electrical
stimuli.
Yet
it
remains
a
challenge
define
underlying
circuitry
that
produces
these
because
contains
wide
variety
of
neuron
classes
whose
patterns
interconnectivity
are
poorly
understood.
Computational
models
locomotion
accordingly
rely
on
untested
assumptions
about
network
element
identity
and
connectivity.
In
this
review,
we
consider
neurons,
their
interconnectivity,
significance
circuit
connections
along
axis
cord.
We
suggest
several
lines
analysis
move
toward
definitive
understanding
network.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июль 5, 2023
Abstract
Peripheral
sensory
neurons
in
the
dorsal
root
ganglion
(DRG)
and
trigeminal
(TG)
are
specialized
to
detect
transduce
diverse
environmental
stimuli
including
touch,
temperature,
pain
central
nervous
system.
Recent
advances
single-cell
RNA-sequencing
(scRNA-seq)
have
provided
new
insights
into
diversity
of
ganglia
cell
types
rodents,
non-human
primates,
humans,
but
it
remains
difficult
compare
transcriptomically
defined
across
studies
species.
Here,
we
built
cross-species
harmonized
atlases
DRG
TG
that
describe
18
neuronal
11
non-neuronal
6
species
19
studies.
We
then
demonstrate
utility
this
reference
atlas
by
using
annotate
newly
profiled
nuclei/cells
from
both
human
highly
regenerative
axolotl.
observe
transcriptomic
profiles
neuron
subtypes
broadly
similar
vertebrates,
expression
functionally
important
neuropeptides
channels
can
vary
notably.
The
resources
data
presented
here
guide
future
comparative
transcriptomics,
simplify
type
nomenclature
differences
studies,
help
prioritize
targets
for
therapy
development.
The
paraventricular
nucleus
of
the
thalamus
(PVT)
is
known
to
regulate
various
cognitive
and
behavioral
processes.
However,
while
functional
diversity
among
PVT
circuits
has
often
been
linked
cellular
differences,
molecular
identity
spatial
distribution
cell
types
remain
unclear.
To
address
this
gap,
here
we
used
single
RNA
sequencing
(snRNA-seq)
identified
five
molecularly
distinct
neuronal
subtypes
in
mouse
brain.
Additionally,
multiplex
fluorescent
situ
hybridization
top
marker
genes
revealed
that
are
organized
by
a
combination
previously
unidentified
gradients.
Lastly,
comparing
our
dataset
with
recently
published
single-cell
atlas
yielded
novel
insight
into
PVT’s
connectivity
cortex,
including
unexpected
innervation
auditory
visual
areas.
This
comparison
also
data
contains
largely
non-overlapping
transcriptomic
map
multiple
midline
thalamic
nuclei.
Collectively,
findings
uncover
unknown
features
anatomical
organization
provide
valuable
resource
for
future
investigations.
npj Parkinson s Disease,
Год журнала:
2024,
Номер
10(1)
Опубликована: Март 19, 2024
Parkinson's
disease
(PD)
is
the
second
most
common
neurodegenerative
worldwide
and
presents
pathologically
with
Lewy
pathology
dopaminergic
neurodegeneration.
contains
aggregated
α-synuclein
(αSyn),
a
protein
encoded
by
SNCA
gene
which
also
mutated
or
duplicated
in
subset
of
familial
PD
cases.
Due
to
its
predominant
presynaptic
localization,
immunostaining
for
results
diffuse
reactivity
pattern,
providing
little
insight
into
types
cells
expressing
αSyn.
As
result,
αSyn
expression-driven
cellular
vulnerability
has
been
difficult
ascertain.
Using
combination
knock-in
mice
that
target
nucleus
(Snca
Cell Reports,
Год журнала:
2024,
Номер
43(2), С. 113718 - 113718
Опубликована: Янв. 30, 2024
How
mechanical
allodynia
following
nerve
injury
is
encoded
in
patterns
of
neural
activity
the
spinal
cord
dorsal
horn
(DH)
remains
incompletely
understood.
We
address
this
mice
using
spared
model
neuropathic
pain
and
vivo
electrophysiological
recordings.
Surprisingly,
despite
dramatic
behavioral
over-reactivity
to
stimuli
injury,
an
overall
increase
sensitivity
or
reactivity
DH
neurons
not
observed.
do,
however,
observe
a
marked
decrease
correlated
firing
patterns,
including
synchrony
stimulus-evoked
firing,
across
DH.
Alterations
temporal
are
recapitulated
by
silencing
parvalbumin+
(PV+)
interneurons,
previously
implicated
allodynia,
as
allodynic
pain-like
behaviors.
These
findings
reveal
decorrelated
network
activity,
driven
alterations
PV+
prominent
feature
suggest
restoration
proper
potential
therapeutic
strategy
treat
chronic
pain.
