Altered brain-wide auditory networks in a zebrafish model of fragile X syndrome

BMC Biology, Год журнала: 2020, Номер 18(1)

Опубликована: Сен. 16, 2020

Abstract Background Loss or disrupted expression of the FMR1 gene causes fragile X syndrome (FXS), most common monogenetic form autism in humans. Although disruptions sensory processing are core traits FXS and autism, neural underpinnings these phenotypes poorly understood. Using calcium imaging to record from entire brain at cellular resolution, we investigated neuronal responses visual auditory stimuli larval zebrafish, using fmr1 mutants model FXS. The purpose this study was alterations networks, brain-wide that underlie aspects autism. Results Combining functional analyses with neurons’ anatomical positions, found −/− animals have normal motion. However, there were several animals. Auditory more plentiful hindbrain structures thalamus. thalamus, torus semicircularis, tegmentum had clusters neurons responded strongly Functional connectivity networks showed inter-regional lower sound intensities (a − 3 6 dB shift) larvae compared wild type. Finally, decoding capacities specific components ascending pathway altered: octavolateralis nucleus within significantly stronger amplitude while telencephalon weaker mutants. Conclusions We demonstrated hypersensitive sound, a 3–6 shift sensitivity, identified four sub-cortical regions and/or greater response strengths stimuli. also constructed an experimentally supported how information may be processed larvae. Our suggests early transmits information, less filtering modulation,

Язык: Английский

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Whole-brain imaging of freely-moving zebrafish

Frontiers in Neuroscience, Год журнала: 2023, Номер 17

Опубликована: Апрель 17, 2023

One of the holy grails neuroscience is to record activity every neuron in brain while an animal moves freely and performs complex behavioral tasks. While important steps forward have been taken recently large-scale neural recording rodent models, single resolution across entire mammalian remains elusive. In contrast larval zebrafish offers great promise this regard. Zebrafish are a vertebrate model with substantial homology brain, but their transparency allows whole-brain recordings genetically-encoded fluorescent indicators at single-neuron using optical microscopy techniques. Furthermore begin show repertoire natural behavior from early age, including hunting small, fast-moving prey visual cues. Until work address bases these behaviors mostly relied on assays where fish was immobilized under microscope objective, stimuli such as were presented virtually. However significant progress has made developing imaging techniques for which not immobilized. Here we discuss recent advances, focusing particularly based light-field microscopy. We also draw attention several outstanding issues remain be addressed increase ecological validity results obtained.

Язык: Английский

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Neural circuits underlying habituation of visually evoked escape behaviors in larval zebrafish

eLife, Год журнала: 2023, Номер 12

Опубликована: Март 14, 2023

Larval zebrafish that are exposed repeatedly to dark looming stimuli will quickly habituate these aversive signals and cease respond with their stereotypical escape swims. A stimulus can be separated into two independent components: one is characterized by an overall spatial expansion, where luminance maintained at the same level, a second, represents dimming within whole visual field in absence of any motion energy. Using specific stimulation patterns isolate components, we first extracted behavioral algorithms dictate how separate information channels interact each other across eyes during habituation process. Concurrent brain wide imaging experiments then permitted construction circuit models suggest existence neural pathways. The channel which responds specifically expanding edges presented contralateral eye relays stem network generate directed escapes. second dimming-specific could either monocular or binocularly responsive, appears inhibit response when activated. We propose this under strong contextual modulation it primarily responsible for incremental silencing successive looming-evoked

Язык: Английский

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The Visual Systems of Zebrafish

Annual Review of Neuroscience, Год журнала: 2024, Номер 47(1), С. 255 - 276

Опубликована: Апрель 25, 2024

The zebrafish visual system has become a paradigmatic preparation for behavioral and systems neuroscience. Around 40 types of retinal ganglion cells (RGCs) serve as matched filters stimulus features, including light, optic flow, prey, objects on collision course. RGCs distribute their signals via axon collaterals to 12 retinorecipient areas in forebrain midbrain. major visuomotor hub, the tectum, harbors nine RGC input layers that combine information multiple features. retinotopic map tectum is locally adapted scene statistics subfield–specific demands. Tectal projections premotor centers are topographically organized according commands. known connectivity more than 20 processing streams allows us dissect cellular basis elementary perceptual cognitive functions. Visually evoked responses, such prey capture or loom avoidance, controlled by dedicated multistation pathways that—at least larva—resemble labeled lines. This architecture serves neuronal code's purpose driving adaptive behavior.

Язык: Английский

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The visuomotor transformations underlying target-directed behavior

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(13)

Опубликована: Март 24, 2025

The visual system can process diverse stimuli and make the decision to execute appropriate behaviors, but it remains unclear where how this transformation takes place. Innate visually evoked behaviors such as hunting, freezing, escape are thought be deeply conserved, have been described in a range of species from insects humans. We found that zebrafish larvae would respond predator-like with immobility bradycardia, both hallmarks head-fixed behavioral paradigm. then imaged while responded different systematically identified driven neurons behaviorally correlated sensorimotor neurons. Our analyses indicate within optic tectum, broadly tuned sensory functionally which specifically during one behavior, indicating contains suitable information for transformation. also four other areas downstream these specific segregation pathways continues areas. While our findings shed light on may integrate inputs, further investigation will required determine regions interact behave is made.

Язык: Английский

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Calcium Imaging and the Curse of Negativity

Frontiers in Neural Circuits, Год журнала: 2021, Номер 14

Опубликована: Янв. 6, 2021

The imaging of neuronal activity using calcium indicators has become a staple modern neuroscience. However, without ground truths, there is real risk missing significant portion the responses. Here, we show that common assumption, non-negativity responses as detected by indicators, biases all levels frequently used analytical methods for these data. From extraction meaningful fluorescence changes to spike inference and analysis inferred spikes, each step risks because assumption non-negativity. We first negative deviations from baseline can exist in activity. Then, use simulated data test three popular algorithms image analysis, CaImAn, suite2p, CellSort, finding suite2p may be best suited large datasets. also tested included Cellsort, well dedicated MLspike CASCADE, found have limitations dealing with inhibited neurons. Among algorithms, FOOPSI, performed on neurons, but even this algorithm spurious spikes upon return signal baseline. As such, new approaches will needed before sensitively accurately further suggest avoiding that, assuming non-negativity, ignore Instead, exploratory step, k-means or PCA example, detect whether are present. Taking steps ensure inhibition, excitation,

Язык: Английский

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Zebrafish as a Mainstream Model for In Vivo Systems Pharmacology and Toxicology

The Annual Review of Pharmacology and Toxicology, Год журнала: 2022, Номер 63(1), С. 43 - 64

Опубликована: Сен. 24, 2022

Pharmacology and toxicology are part of a much broader effort to understand the relationship between chemistry biology. While biomedicine has necessarily focused on specific cases, typically direct human relevance, there real advantages in pursuing more systematic approaches characterizing how health disease influenced by small molecules other interventions. In this context, zebrafish is now established as representative screenable vertebrate and, through ongoing advances available scale genome editing automated phenotyping, beginning address systems-level solutions some biomedical problems. The addition efforts integrate information content across preclinical model organisms incorporation rigorous analytics, including closed-loop deep learning, will facilitate create systems pharmacology with ability continuously optimize chemical biological interactions around societal needs. review, we outline progress toward goal.

Язык: Английский

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Structure and function in artificial, zebrafish and human neural networks

Physics of Life Reviews, Год журнала: 2023, Номер 45, С. 74 - 111

Опубликована: Апрель 25, 2023

Язык: Английский

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Spatial evolution of the proton-coupled Mott transition in correlated oxides for neuromorphic computing

Science Advances, Год журнала: 2024, Номер 10(22)

Опубликована: Май 31, 2024

The proton-electron coupling effect induces rich spectrums of electronic states in correlated oxides, opening tempting opportunities for exploring novel devices with multifunctions. Here, via modest Pt-aided hydrogen spillover at room temperature, amounts protons are introduced into SmNiO 3 -based devices. In situ structural characterizations together first-principles calculation reveal that the local Mott transition is reversibly driven by migration and redistribution predoped protons. accompanying giant resistance change results excellent memristive behaviors under ultralow electric fields. Hierarchical tree-like memory states, an instinct displayed bio-synapses, further realized spatially varying proton concentration pulses, showing great promise artificial neural networks solving intricate problems. Our research demonstrates direct effective control evolution using extremely low field, offering alternative pathway modifying functionalities oxides constructing low–power consumption intelligent network circuits.

Язык: Английский

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Broad frequency sensitivity and complex neural coding in the larval zebrafish auditory system

Current Biology, Год журнала: 2021, Номер 31(9), С. 1977 - 1987.e4

Опубликована: Март 2, 2021

Язык: Английский

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