Engineering Biological Nanopore Approaches toward Protein Sequencing

ACS Nano, Год журнала: 2023, Номер 17(17), С. 16369 - 16395

Опубликована: Июль 25, 2023

Biotechnological innovations have vastly improved the capacity to perform large-scale protein studies, while methods we for identifying and quantifying individual proteins are still inadequate sequencing at single-molecule level. Nanopore-inspired systems devoted understanding how single molecules behave been extensively developed applications in genome sequencing. These nanopore emerging as prominent tools identification, detection, analysis, suggesting realistic prospects novel This review summarizes recent advances biological sensors toward sequencing, from identification of amino acids controlled translocation peptides proteins, with attention focused on device algorithm development delineation molecular mechanisms aid simulations. Specifically, aims offer recommendations advancement nanopore-based an engineering perspective, highlighting need collaborative efforts across multiple disciplines. should include chemical conjugation, engineering, simulation, machine-learning-assisted electronic fabrication enable practical implementation real-world scenarios.

Язык: Английский

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Identification of Conformational Variants for Bradykinin Biomarker Peptides from a Biofluid Using a Nanopore and Machine Learning

ACS Nano, Год журнала: 2023, Номер 18(1), С. 539 - 550

Опубликована: Дек. 22, 2023

There is a current need to develop methods for the sensitive detection of peptide biomarkers in complex mixtures molecules, such as biofluids, enable early disease detection. Moreover, our knowledge, there currently no method capable identifying different conformations biomarker differing by single amino acid. Single-molecule nanopore sensing promises provide this level resolution. In order be able identify these differences biofluid serum, it necessary carefully characterize electrical parameters obtain specific signatures each population observed. We are interested here family biomarkers, kinins bradykinin and des-Arg9 bradykinin, that involved many disabling pathologies (allergy, asthma, angioedema, sepsis, or cancer). show proof concept direct identification serum at single-molecule using protein nanopore. Each exhibits two unique attributed bulk. The same found allowing their discrimination mixture biofluid. To extend utility experimental results, we developed principal component analysis approach define most relevant identification. Finally, used semisupervised classification assign event type physiological concentration. future, scale powerful coupled with machine learning will facilitate quantification other clinically from biofluids.

Язык: Английский

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Recent Advances in Nanopore Technology for Copper Detection and Their Potential Applications

Nanomaterials, Год журнала: 2023, Номер 13(9), С. 1573 - 1573

Опубликована: Май 8, 2023

Recently, nanopore technology has emerged as a promising technique for the rapid, sensitive, and selective detection of various analytes. In particular, use nanopores copper ions attracted considerable attention due to their high sensitivity selectivity. This review discusses principles its advantages over conventional techniques detection. It covers different types used detection, including biological synthetic nanopores, mechanisms detect ions. Furthermore, this provides an overview recent advancements in development new materials, improvements signal amplification, integration with other analytical methods enhanced accuracy. Finally, we summarize extensive applications, current challenges, future perspectives using highlighting need further research field optimize performance applicability technique.

Язык: Английский

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Narrowing Signal Distribution by Adamantane Derivatization for Amino Acid Identification Using an α-Hemolysin Nanopore

Nano Letters, Год журнала: 2024, Номер 24(5), С. 1494 - 1501

Опубликована: Янв. 24, 2024

The rapid progress in nanopore sensing has sparked interest protein sequencing. Despite recent notable advancements amino acid recognition using nanopores, chemical modifications usually employed this process still need further refinements. One of the challenges is to enhance specificity avoid downstream misidentification acids. By employing adamantane label proteinogenic acids, we developed an approach fingerprint individual acids wild-type α-hemolysin nanopore. unique structure adamantane-labeled (ALAAs) improved spatial resolution, resulting distinctive current signals. Various parameters were explored a machine-learning algorithm and achieved validation accuracy 81.3% for distinguishing nine selected Our results not only advance effort single-molecule characterization nanopores but also offer potential platform studying intrinsic variant structures molecules.

Язык: Английский

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Classification and applications of nanomaterials in vitro diagnosis

Heliyon, Год журнала: 2024, Номер 10(11), С. e32314 - e32314

Опубликована: Июнь 1, 2024

With the rapid development of clinical diagnosis and treatment, many traditional conventional in vitro technologies are unable to meet demands medicine development. In this situation, nanomaterials rapidly developing widely used field diagnosis. Nanomaterials have distinct size-dependent physical or chemical properties, their optical, magnetic, electrical, thermal, biological properties can be modulated at nanoscale by changing size, shape, composition, surface functional groups, particularly because they a larger specific area than macromaterials. They provide an amount space modify different molecules on surface, allowing them detect small substances, nucleic acids, proteins, microorganisms. Combining with is expected result lower detection limits, higher sensitivity, stronger selectivity. review, we will discuss classfication some common nanomaterials, as well applications protein, other aspect analysis for diagnosis, especially aging-related nanodiagnostics. Finally, it summarized guidelines

Язык: Английский

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Selective Single-Molecule Nanopore Detection of mpox A29 Protein Directly in Biofluids

Nano Letters, Год журнала: 2023, Номер 23(24), С. 11438 - 11446

Опубликована: Дек. 5, 2023

Single-molecule antigen detection using nanopores offers a promising alternative for accurate virus testing to contain their transmission. However, the selective and efficient identification of small viral proteins directly in human biofluids remains challenge. Here, we report nanopore sensing strategy based on customized DNA molecular probe that combines an aptamer antibody enhance single-molecule mpox (MPXV) A29 protein, protein with M.W. ca. 14 kDa. The formation aptamer–target–antibody sandwich structures enables targets when translocating through nanopore. This technique can accurately detect limit ∼11 fM distinguish MPXV from vaccinia A27 (a difference only four amino acids) Varicella Zoster Virus (VZV) biofluids. simplicity, high selectivity, sensitivity this approach have potential contribute diagnosis viruses point-of-care settings.

Язык: Английский

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Biosensing meets click chemistry: A promising combination for analysis of food hazard factors

Coordination Chemistry Reviews, Год журнала: 2024, Номер 520, С. 216137 - 216137

Опубликована: Авг. 12, 2024

Язык: Английский

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Neuron-Inspired Nanofluidic Biosensors for Highly Sensitive and Selective Imidacloprid Detection

ACS Sensors, Год журнала: 2023, Номер 8(9), С. 3428 - 3434

Опубликована: Авг. 8, 2023

Pesticides have caused concerns about food safety due to their residual effects in vegetables and fruits. Imidacloprid, as the frequently used neonicotinoid pesticide, could harm cardiovascular respiratory function cause reproductive toxicity humans. Therefore, reliable methods for portable, selective, rapid detection are desirable develop. Herein, we report a neuron-inspired nanofluidic biosensor based on tyrosine-modified artificial nanochannel sensitively detecting imidacloprid. The functional tyrosine is modified outer surface of porous anodic aluminum oxide rapidly capture imidacloprid through π-π interactions hydrogen bonds. integrated has wide concentration range from 10-8 10-4 g/mL with an ultralow limit 6.28 × 10-9 g/mL, which outperforms state-of-the-art sensors. This work provides new perspective residues well other hazardous pesticide environmental samples.

Язык: Английский

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Single-molecule profiling of per- and polyfluoroalkyl substances by cyclodextrin mediated host-guest interactions within a biological nanopore

Science Advances, Год журнала: 2024, Номер 10(45)

Опубликована: Ноя. 6, 2024

Biological nanopores are increasingly used in molecular sensing due to their single-molecule sensitivity. The detection of per- and polyfluoroalkyl substances (PFAS) like perfluorooctanoic acid perfluorooctane sulfonic is critical environmental prevalence toxicity. Here, we investigate selective interactions between PFAS four cyclodextrin (CD) variants (α-, β-, γ-, 2-hydroxypropyl-γ-CD) within an α-hemolysin nanopore. We demonstrate that molecules can be electrochemically sensed by interacting with a γ-CD Using HP-γ-CDs increased steric resistance, identify homologs the perfluoroalkyl carboxylic families detect common drinking water at 0.4 2 parts per million levels, which further lowered 400 trillion sample preconcentration. Molecular dynamics simulations reveal underlying chemical mechanism PFAS-CD interactions. These insights pave way toward nanopore-based situ promises protection against pollution.

Язык: Английский

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Chromato-kinetic fingerprinting enables multiomic digital counting of single disease biomarker molecules

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 4, 2025

Abstract Early and personalized intervention in complex diseases requires robust molecular diagnostics, yet the simultaneous detection of diverse biomarkers—microRNAs (miRNAs), mutant DNAs, proteins—remains challenging due to low abundance preprocessing incompatibilities. We present Biomarker Single-molecule Chromato-kinetic multi-Omics Profiling Enumeration (Bio-SCOPE), a next-generation, triple-modality, multiplexed platform that integrates both chromatic kinetic fingerprinting for profiling through digital encoding. Bio-SCOPE achieves femtomolar sensitivity, single-base mismatch specificity, minimal matrix interference, enabling precise, parallel quantification up six biomarkers single sample with single-molecule resolution. demonstrate its versatility accurately detecting low-abundance miRNA signatures from human tissues, identifying upregulated miRNAs plasma prostate cancer patients, measuring elevated interleukin-6 (IL-6) hsa-miR-21 levels cytokine release syndrome patients. By seamlessly integrating multiomic biomarker panels on unified, high-precision platform, provides transformative tool diagnostics precision medicine.

Язык: Английский

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