Nature Synthesis, Год журнала: 2024, Номер 3(6), С. 673 - 674
Опубликована: Март 27, 2024
Язык: Английский
Опубликована: Март 18, 2024
Bio-soft matter droplets formed via liquid-liquid phase separation (LLPS) of biopolymers have been found in living cells. Synthetic LLPS recently employed nanobiotechnology for artificial cell construction, molecular robotics, computing, diagnosis, and therapeutics. Controlling the dynamics bio-soft is essential developing such bio-inspired functional systems because maintain their functions based on temporally controlled biomolecular reactions assemblies. Recently, revealed; however, temporal control has not yet achieved. This paper reports DNA-based (DNA droplets). We demonstrate timing-controlled division DNA droplet-based cells time-delayed triggers regulated by non-equilibrium chemical reactions. also investigated it using a reaction-diffusion model. release order multiple triggers, resulting multistep droplet division, that is, pathway reaction landscape. Finally, we an application cells: computing element to compare concentrations microRNA sequences (called comparators). believe will promote design more dynamic cells/molecular robots sophisticated biomedical applications.
Язык: Английский
Процитировано
3
Colloids and Surfaces A Physicochemical and Engineering Aspects, Год журнала: 2024, Номер 691, С. 133891 - 133891
Опубликована: Апрель 2, 2024
Язык: Английский
Процитировано
3
Chemical Science, Год журнала: 2024, Номер 15(19), С. 7092 - 7103
Опубликована: Янв. 1, 2024
The mechanisms through which environmental conditions affect the expression of interconnected species is a key step to comprehending principles underlying complex chemical processes. In Nature, modifications triggered by environment have major impact on structure and function biomolecules regulate different reaction pathways. Yet, minimalistic artificial systems implementing related adaptation behaviours remain barely explored. hydrolysis amino acid methyl esters their corresponding acids leads drastic change in pKa (ca. 7 9, respectively) that protonates free group at physiological conditions. Dynamic covalent libraries (DCvLs) based aldehydes respond such lead constitutional adaptation. Each studied experiences DCvL conversion allowing for constituent selection due silencing zwitterionic towards imine formation. selective action enzymes DCvLs results states with simplified distributions transient chirality. When additional components (i.e., scavengers) are not affected introduced into dynamic libraries, ester induces up-regulation constituents made these scavenging components. systems, distribution resolved from scrambled mixture imines state characterized predominance single aldimine. Remarkably, although final display higher "simplexity", present an increased complexity allows emergence organized structures [micelle formation] [up-regulation two antagonistic constituents]. This reactive site inhibition remote modification resembles scenario found some regulation activity proximal post-translational modifications.
Язык: Английский
Процитировано
3
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Март 5, 2024
Abstract Proteinaceous inclusions formed by C9orf72 derived dipeptide-repeat (DPR) proteins are a histopathological hallmark in ~50% of familial amyotrophic lateral sclerosis/frontotemporal dementia (ALS/FTD) cases. However DPR aggregation/inclusion formation could not be efficiently recapitulated cell models for four out five DPRs. In this study, using optogenetics, we achieved chemical-free poly-PR condensation/aggregation cultured cells, with spatial and temporal control. Strikingly, nuclear condensates had anisotropic, hollow-centre appearance, resembling anisosomes aberrant TDP-43 species, their growth was limited RNA. These induced abnormal granulation the nucleus without activation stress response. Cytoplasmic aggregates that under prolonged light stimulation were more persistent than its condensates, selectively sequestered demixed state surrounded spontaneous granules. Our data suggest anisotropic condensation nucleus, causative dysfunction, may constitute an early pathological event C9-ALS/FTD. Anisosome-type represent common cellular pathology neurodegeneration previously thought. Highlights - Optogenetics can used to model cells. Opto-PR forms hollow RNA limits fusion. leads stress-independent nucleus. assemblies sequester TDP-43. Graphical abstract
Язык: Английский
Процитировано
2
Nature Synthesis, Год журнала: 2024, Номер 3(6), С. 673 - 674
Опубликована: Март 27, 2024
Язык: Английский
Процитировано
2