A contextual fear conditioning paradigm in head-fixed mice exploring virtual reality. DOI Creative Commons
Seetha Krishnan, Can Dong,

Heather Ratigan

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 27, 2024

ABSTRACT Contextual fear conditioning is a classical laboratory task that tests associative memory formation and recall. Techniques such as multi-photon microscopy holographic stimulation offer tremendous opportunities to understand the neural underpinnings of these memories. However, techniques generally require animals be head-fixed. There are few paradigms test contextual in head-fixed mice, none where behavioral outcome following freezing, most common measure freely moving animals. To address this gap, we developed paradigm mice using virtual reality (VR) environments. We designed an apparatus deliver tail shocks (unconditioned stimulus, US) while navigated VR environment (conditioned CS). The acquisition was tested when were reintroduced shock-paired day. three different variations and, all them, observed increased conditioned response characterized by freezing behavior. This especially prominent during first trial environment, compared neutral received no shocks. Our results demonstrate can VR, discriminate between feared context, display response, similar behaving Furthermore, two-photon microscope, imaged from large populations hippocampal CA1 neurons before, during, conditioning. findings reconfirmed those literature on animals, showing place cells undergo remapping show narrower fields approach offers new study mechanisms underlying formation, recall, extinction As preparation compatible with stimulation, it enables long-term tracking manipulation throughout distinct stages provides subcellular resolution for investigating axonal, dendritic, synaptic dynamics real-time.

Язык: Английский

A thalamic-hippocampal CA1 signal for contextual fear memory suppression, extinction, and discrimination DOI Creative Commons

Heather Ratigan,

Seetha Krishnan, Shai Smith

и другие.

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Окт. 24, 2023

Abstract The adaptive regulation of fear memories is a crucial neural function that prevents inappropriate expression. Fear can be acquired through contextual conditioning (CFC) which relies on the hippocampus. thalamic nucleus reuniens (NR) necessary to extinguish and innervates hippocampal CA1. However, role NR-CA1 pathway in unknown. We developed head-restrained virtual reality CFC paradigm, demonstrate mice acquire context-dependent responses. found inhibiting following lengthens duration fearful freezing epochs, increases generalization, delays extinction. Using vivo imaging, we recorded NR-axons innervating CA1 become tuned CFC. conclude actively suppresses by disrupting memory retrieval during behavior, process also reduces generalization accelerates

Язык: Английский

Процитировано

27
Neural circuits for the adaptive regulation of fear and extinction memory DOI Creative Commons
Samantha L. Plas, Tuğçe Tuna, Hugo Bayer

и другие.

Frontiers in Behavioral Neuroscience, Год журнала: 2024, Номер 18

Опубликована: Фев. 2, 2024

The regulation of fear memories is critical for adaptive behaviors and dysregulation these processes implicated in trauma- stress-related disorders. Treatments disorders include pharmacological interventions as well exposure-based therapies, which rely upon extinction learning. Considerable attention has been directed toward elucidating the neural mechanisms underlying In this review, we will discuss historic discoveries emerging evidence on memories. We focus circuits regulating acquisition Pavlovian conditioning rodent models, particularly role medial prefrontal cortex hippocampus contextual control extinguished also consider new work revealing an important thalamic nucleus reuniens modulation prefrontal-hippocampal interactions learning memory. Finally, explore effects stress circuit clinical implications findings.

Язык: Английский

Процитировано

14
CA1 Engram Cell Dynamics Before and After Learning DOI Creative Commons
Amy Monasterio, Caitlin Lienkaemper,

Siria Coello

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 20, 2024

Summary A fundamental question in neuroscience is how memory formation shapes brain activity at the level of populations neurons. Recent studies hippocampal ‘engram’ cells, identified by immediate-early genes (IEGs) induced learning, propose that these act as a neuronal substrate for storage. The current framework engram proposes cells join ensembles based on increased intrinsic excitability, and after initial they co-activate to support retrieval. However, direct evidence population dynamics evolve across learning limited. Here we combined activity-dependent genetic tagging two-photon calcium imaging characterize CA1 before learning. We observed spontaneous two days predicted tagging, consistent with model which fluctuations bias into forming assemblies. Surprisingly, were unable detect rates or pairwise correlations amongst tagged neurons These results computational network models incorporate strong specific inhibitory connections, supporting idea excitatory/inhibitory balance may play key role dynamics. Together highlight potential slow time scale excitability driving suggest excitatory-inhibitory regulate cell co-activation. Highlights Tracked Fos Spontaneous predicts TetTagging. No changes TetTagged Modeling suggests E/I regulates

Язык: Английский

Процитировано

14
Projections from thalamic nucleus reuniens to medial septum enable extinction of remote fear memory DOI Creative Commons
Kamil F. Tomaszewski, Magdalena Ziółkowska, Kacper Łukasiewicz

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 20, 2024

Abstract Aversive experiences lead to the formation of long-lasting memories. Despite need better understand how enduring fear memories can be attenuated, underlying brain circuits remain largely unknown. In this study, employing a combination genetic manipulations, neuronal circuit mapping, and chemogenetics in mice, we identify new projection from thalamic nucleus reuniens (RE) medial septum (MS), show that is involved extinction remote (30-day old), but not recent (1-day These findings provide first functional description RE→MS highlight significance thalamo-septal regions memory organization as function age, phenomenon known systems consolidation.

Язык: Английский

Процитировано

5
Projections from thalamic nucleus reuniens to hippocampal CA1 area participate in context fear extinction by affecting extinction-induced molecular remodeling of excitatory synapses DOI Creative Commons
Magdalena Ziółkowska, Narges Sotoudeh, Anna Cały

и другие.

eLife, Год журнала: 2025, Номер 13

Опубликована: Янв. 23, 2025

The ability to extinguish contextual fear in a changing environment is crucial for animal survival. Recent data support the role of thalamic nucleus reuniens (RE) and its projections dorsal hippocampal CA1 area (RE→dCA1) this process. However, it remains poorly understood how RE impacts dCA1 neurons during extinction (CFE). Here, we reveal that RE→dCA1 pathway contributes by affecting CFE-induced molecular remodeling excitatory synapses. Anatomical tracing chemogenetic manipulation mice demonstrate form synapses regulate synaptic transmission stratum oriens (SO) lacunosum-moleculare (SLM) area, but not radiatum (SR). We also observe CFE-specific structural changes expression scaffold protein, PSD-95, both strata innervated RE, SR. Interestingly, only SLM are specific dendrites RE. To further projection CFE, brief inhibition CFE session persistently impairs formation memory PSD-95 levels SLM. Thus, our indicate participates regulating

Язык: Английский

Процитировано

0
A contextual fear conditioning paradigm in head-fixed mice exploring virtual reality DOI Open Access
Seetha Krishnan, Can Dong,

Heather Ratigan

и другие.

Опубликована: Фев. 14, 2025

Contextual fear conditioning is a classical laboratory task that tests associative memory formation and recall. Techniques such as multi-photon microscopy holographic stimulation offer tremendous opportunities to understand the neural underpinnings of these memories. However, techniques generally require animals be head-fixed. There are few paradigms test contextual in head-fixed mice, none where behavioral outcome following freezing, most common measure freely moving animals. To address this gap, we developed paradigm mice using virtual reality (VR) environments. We designed an apparatus deliver tail shocks (unconditioned stimulus, US) while navigated VR environment (conditioned CS). The acquisition was tested when were reintroduced shock-paired day. three different variations and, all them, observed increased conditioned response characterized by freezing behavior. This especially prominent during first trial environment, compared neutral received no shocks. Our results demonstrate can VR, discriminate between feared context, display response, similar behaving Furthermore, two-photon microscope, imaged from large populations hippocampal CA1 neurons before, during, conditioning. findings reconfirmed those literature on animals, showing place cells undergo remapping show narrower fields approach offers new study mechanisms underlying formation, recall, extinction As preparation compatible with stimulation, it enables long-term tracking manipulation throughout distinct stages provides subcellular resolution for investigating axonal, dendritic, synaptic dynamics real-time.

Язык: Английский

Процитировано

0
A contextual fear conditioning paradigm in head-fixed mice exploring virtual reality DOI Open Access
Seetha Krishnan, Can Dong,

Heather Ratigan

и другие.

Опубликована: Фев. 14, 2025

Contextual fear conditioning is a classical laboratory task that tests associative memory formation and recall. Techniques such as multi-photon microscopy holographic stimulation offer tremendous opportunities to understand the neural underpinnings of these memories. However, techniques generally require animals be head-fixed. There are few paradigms test contextual in head-fixed mice, none where behavioral outcome following freezing, most common measure freely moving animals. To address this gap, we developed paradigm mice using virtual reality (VR) environments. We designed an apparatus deliver tail shocks (unconditioned stimulus, US) while navigated VR environment (conditioned CS). The acquisition was tested when were reintroduced shock-paired day. three different variations and, all them, observed increased conditioned response characterized by freezing behavior. This especially prominent during first trial environment, compared neutral received no shocks. Our results demonstrate can VR, discriminate between feared context, display response, similar behaving Furthermore, two-photon microscope, imaged from large populations hippocampal CA1 neurons before, during, conditioning. findings reconfirmed those literature on animals, showing place cells undergo remapping show narrower fields approach offers new study mechanisms underlying formation, recall, extinction As preparation compatible with stimulation, it enables long-term tracking manipulation throughout distinct stages provides subcellular resolution for investigating axonal, dendritic, synaptic dynamics real-time.

Язык: Английский

Процитировано

0
Cognitive dysfunction in early experimental metabolic dysfunction-associated steatotic liver disease is associated with systemic inflammation and neuroinflammation DOI Creative Commons
Kristoffer Kjærgaard, Anne Catrine Daugaard Mikkelsen, Anne M. Landau

и другие.

JHEP Reports, Год журнала: 2023, Номер 6(3), С. 100992 - 100992

Опубликована: Дек. 21, 2023

Cognitive dysfunction is an increasingly recognised manifestation of metabolic dysfunction-associated steatotic liver disease (MASLD), but the mechanistic link remains unclear. The aim this study was to investigate hypothesis that experimental MASLD leads cognitive via systemic inflammation and neuroinflammation.

Язык: Английский

Процитировано

10
In relentless pursuit of the white whale: A role for the ventral midline thalamus in behavioral flexibility and adaption? DOI Creative Commons

Élodie Panzer,

Isabella Guimares-Olmo,

Anne Pereira de Vasconcelos

и другие.

Neuroscience & Biobehavioral Reviews, Год журнала: 2024, Номер 163, С. 105762 - 105762

Опубликована: Июнь 12, 2024

The reuniens (Re) nucleus is located in the ventral midline thalamus. It has fostered increasing interest, not only for its participation a variety of cognitive functions (e.g., spatial working memory, systemic consolidation, reconsolidation, extinction fear or generalization), but also neuroanatomical positioning as bidirectional relay between prefrontal cortex (PFC) and hippocampus (HIP). In this review we compile discuss recent studies having tackled possible implication Re behavioral flexibility, major PFC-dependent executive function controlling goal-directed behaviors. Experiments considered explored role perseveration, reversal learning, extinction, set-shifting. They point to contribution mainly by connections with PFC, possibly those hippocampus, even amygdala, at least fear-related behavior. As such, could be crucial crossroad supporting PFC-orchestrated ability cope new, potentially unpredictable environmental contingencies, thus flexibility adaption.

Язык: Английский

Процитировано

3
Periplaneta Americana (L.) extract activates the ERK/CREB/BDNF pathway to promote post-stroke neuroregeneration and recovery of neurological functions in rats DOI

Jiangyan Rao,

Hongpu Li, Haonan Zhang

и другие.

Journal of Ethnopharmacology, Год журнала: 2023, Номер 321, С. 117400 - 117400

Опубликована: Ноя. 11, 2023

Язык: Английский

Процитировано

7