Ergothioneine improves healthspan of aged animals by enhancing cGPDH activity through CSE-dependent persulfidation

Cell Metabolism, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Neurospora intermedia from a traditional fermented food enables waste-to-food conversion

Nature Microbiology, Год журнала: 2024, Номер 9(10), С. 2666 - 2683

Опубликована: Авг. 29, 2024

Abstract Fungal fermentation of food and agricultural by-products holds promise for improving sustainability security. However, the molecular basis fungal waste-to-food upcycling remains poorly understood. Here we use a multi-omics approach to characterize oncom, fermented traditionally produced from soymilk in Java, Indonesia. Metagenomic sequencing samples small-scale producers Western Java indicated that fungus Neurospora intermedia dominates oncom. Further transcriptomic, metabolomic phylogenomic analysis revealed oncom-derived N. utilizes pectin cellulose degradation during belongs genetically distinct subpopulation associated with human-generated by-products. Finally, found grew on diverse such as fruit vegetable pomace plant-based milk waste, did not encode mycotoxins, could create foods were positively perceived by consumers outside These results showcase traditional significance future potential creating delicious nutritious readily available

Язык: Английский

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Halophilic Fungi—Features and Potential Applications

Microorganisms, Год журнала: 2025, Номер 13(1), С. 175 - 175

Опубликована: Янв. 15, 2025

Extremophiles are of significant scientific interest due to their unique adaptation harsh environmental conditions and potential for diverse biotechnological applications. Among these extremophiles, filamentous fungi adapted high-salt environments represent a new valuable source enzymes, biomolecules, biomaterials. While most studies on halophiles have focused bacteria, reports remain limited. This review compiles information about salt-adapted details distribution, mechanisms, applications in various societal areas. Understanding the adaptive mechanisms halophilic not only sheds light biology extremophilic but also leads promising applications, including development salt-tolerant enzymes strategies bioremediation saline habitats. To fully realize this potential, comprehensive understanding ecology, diversity physiology is crucial. In addition, survival important astrobiology. The highlighted.

Язык: Английский

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Recent advances in the application of CRISPR/Cas-based gene editing technology in Filamentous Fungi

Biotechnology Advances, Год журнала: 2025, Номер 81, С. 108561 - 108561

Опубликована: Март 12, 2025

Язык: Английский

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Ergothioneine, a New Acrolein Scavenger at Elevated Temperature

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 22, 2025

Acrolein (ACR) present in vivo and vitro can damage proteins DNA, linking it to various chronic diseases. In this paper, ergothioneine (EGT), abundant edible mushrooms, has been studied for its ability trap ACR reaction pathway with at high temperatures using ultraperformance liquid chromatography–mass spectrometry (UPLC–MS/MS). We synthesized the adducts (EGT-ACR-1 EGT-ACR-2), elucidating their structure site through HRMS nuclear magnetic resonance. Subsequently, we proved synergistic trapping effect on of EGT when combined γ-aminobutyric (GABA) disodium 5′-guanylate (GMP) binary or ternary employing Chou–Talalay methods. Quantitative analysis revealed that GABA GMP enhanced EGT's form additional adducts. Moreover, Boletus eduli Volvariella volvacea, as carriers EGT, GABA, GMP, show a marked generated during baking cookies/cakes. Our finding suggested whether standalone compound derived from could act potential capturer (including single multiple uses) baked food.

Язык: Английский

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Mushroom mycelia as sustainable alternative proteins for the production of hybrid cell‐cultured meat: A review

Journal of Food Science, Год журнала: 2025, Номер 90(2)

Опубликована: Фев. 1, 2025

World agriculture endures an immense challenge in feeding the world's growing population face of several productivity and environmental threats. Yet, demand for alternative protein sources is rapidly increasing as a result growth, including health ethical concerns associated with meat consumption. Edible mushroom species contain high composition protein, fiber, vitamins, variety minerals, are regarded sufficient food products. Pleurotus genus one most extensively studied edible fungi due to its exceptional physical, chemical, biological, enzymatic properties. The assessment on effects vitro culture media composition, carbon nitrogen sources, pH, temperature all necessary enhancing mycelial biomass growth production. Mycoprotein fungal-derived source has been identified more sustainable healthier substitute fibrous structure, nutritional value, unique functional profile. Its distinctive production method results much lower water footprint than traditional farming methods. A systemic transition from cellular using cell-cultivation methods create animal products proposed initiated. This review can provide overview various processes involved usage mycelium hybrid cell-cultured

Язык: Английский

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Discovery, characterization, and application of chromosomal integration sites for stable heterologous gene expression in Rhodotorula toruloides

Metabolic Engineering, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

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Black yeasts are efficient heterologous hosts of a wide range of fungal polyketides

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Фев. 20, 2025

Fungal natural products, including polyketides, are a rich source of bioactive molecules. Their biosynthetic enzymes encoded within gene clusters, which often activated by specific environmental conditions. As result, many products not produced under standard laboratory Heterologous expression bypasses native regulation, enabling systematic approach for polyketide discovery. The most widely used fungal hosts product production Saccharomycetales yeasts, and filamentous Eurotiomycetes. Yeasts highly tractable but have narrow scope due to their limited secondary metabolism, while Eurotiomycetes richer metabolism more difficult engineer. In this work, we established two yeasts the genera Exophiala and Knufia as novel heterologous broad range polyketides. These combine genetic tractability yeast with metabolic robustness fungi. We developed engineering tools precise integration genome editing, allowing us heterologously express five synthases different domain architectures, one involved in biosynthesis previously undescribed 2-pyridone. Our findings demonstrate that these can efficiently produce complex paving way synthase engineering.

Язык: Английский

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Innovative mycelium‐based food: Advancing One Health through nutritional insights and environmental sustainability

Comprehensive Reviews in Food Science and Food Safety, Год журнала: 2025, Номер 24(2)

Опубликована: Март 1, 2025

One Health states that human health is indispensably connected with animal and environmental health. Traditionally, humans consume meat to get animal-sourced protein in order fulfill the goal of maintaining physical However, productions seem already not supportive current food system when facing enormous population, which potentially enhances development alternative industry. In 2023, about 28.9% world populations were exposed moderate or severe insecurity. Meanwhile, intensive livestock farming has caused serious issues on atmospheric pollution, land occupation, water consumption. To alleviate such stresses, proteins come spotlight. Currently, plant-based protein, cell-cultured mycelium-based (MBP), algal-based are main categories proteins, among MBP acquiring more popularity as a nutritional, eco-friendly, sustainable source protein. More than provider, (MBF) abundant various nutrients bioactive compounds make great contribution Unlike conventional husbandry, mycelium production puts less stress environment can grow waste substrates, recycling wastes used be abandoned. This review aims establish comprehensive understanding characteristics, nutritional value, functional properties, potential applications MBFs. Additionally, it explores advancements technologies, challenges, future research directions optimize commercialization MBF products.

Язык: Английский

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Redesigning and rethinking genetic circuits: the potential of transcriptional rewiring in filamentous fungi

Current Opinion in Biotechnology, Год журнала: 2025, Номер 93, С. 103301 - 103301

Опубликована: Апрель 4, 2025

Язык: Английский

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