STING Drives Pyroptosis and Exacerbates Intestinal Ischemia-Reperfusion Injury DOI Creative Commons
Ke Ding, Yiguo Zhang, Lele Zhang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 15, 2024

Abstract This study aimed to investigate the role of STING (Stimulator Interferon Genes) in intestinal ischemia-reperfusion (IIR) injury, focusing on its impact mitochondrial function, pyroptosis, and inflammation. The utilized both vivo vitro models, including a mouse model IIR organoids subjected hypoxia-reoxygenation (HR). We assessed effects pharmacological inhibition using H.151 knockout injury markers, histological damage, pro-inflammatory cytokine levels, morphology pyroptosis markers. Our findings revealed that significantly upregulated expression epithelium, coinciding with increased permeability, elevated production (IL-1β) (p < 0.05), evidence dysfunction, characterized by reduced ATP disrupted 0.01). Importantly, treatment attenuated IIR-induced damage 0.01), levels preserved function Furthermore, we observed significant reduction as evidenced decreased cleaved gasdermin D H.151-treated groups 0.05). identifies critical mediator likely through activation pathways leading dysfunction pyroptosis. suggest targeting represents promising therapeutic strategy for mitigating following ischemia-reperfusion.

Язык: Английский

Eicosapentaenoic acid enhances intestinal stem cell-mediated colonic epithelial regeneration by activating the LSD1-WNT signaling pathway DOI Creative Commons
Dan Wang, Nayiyuan Wu, Pei Li

и другие.

Journal of Advanced Research, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

19
Advancements and Challenges of R-loops in Cancers: Biological Insights and Future Directions DOI
Dengxiong Li, Fanglin Shao, Xinrui Li

и другие.

Cancer Letters, Год журнала: 2024, Номер 610, С. 217359 - 217359

Опубликована: Ноя. 27, 2024

Язык: Английский

Процитировано

4
Identification and Construction of a R-loop Mediated Diagnostic Model and Associated Immune Microenvironment of COPD through Machine Learning and Single-Cell Transcriptomics DOI Creative Commons

Jianing Lin,

Yayun Nan,

Jingyi Sun

и другие.

Inflammation, Год журнала: 2025, Номер unknown

Опубликована: Янв. 11, 2025

Chronic obstructive pulmonary disease (COPD) is a prevalent chronic inflammatory airway with high incidence and significant burden. R-loops, functional chromatin structure formed during transcription, are closely associated inflammation due to its aberrant formation. However, the role of R-loop regulators (RLRs) in COPD remains unclear. Utilizing both bulk transcriptome data single-cell RNA sequencing data, we assessed diverse expression patterns RLRs lung tissues patients. A lower score was found patients neutrophils. 12 machine learning algorithms (150 combinations) identified 14 hub (CBX8, EHD4, HDLBP, KDM6B, NFAT5, NLRP3, NUP214, PAFAH1B3, PINX1, PLD1, POLB, RCC2, RNF213, VIM) COPD. RiskScore based on two distinct subtypes. Patient groups at risk (low scores) had higher immune increase neutrophils their microenvironment compared low-risk groups. PD-0325901 QL-X-138 represent prospective treatments for high-risk score) (high Finally, RT-PCR experiments confirmed differences 8 (EHD4, mice tissue. R-loops significantly contribute development constructing predictive models may provide crucial insight into personalized treatment strategies

Язык: Английский

Процитировано

0
The hidden weavers: A review of DNA/RNA R-loops in stem cell biology and therapeutic potential DOI
Xu Chen,

Zizhao Mai,

Yucheng Zheng

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 297, С. 139895 - 139895

Опубликована: Янв. 14, 2025

Язык: Английский

Процитировано

0
R-loop homeostasis in genome dynamics, gene expression and development DOI
Paula Aguilera, Andrés Aguilera

Current Opinion in Genetics & Development, Год журнала: 2025, Номер 92, С. 102325 - 102325

Опубликована: Март 4, 2025

Язык: Английский

Процитировано

0
The emerging role of intestinal stem cells in ulcerative colitis DOI Creative Commons

Siqing Chen,

Qin Zhang, Sainan Zhou

и другие.

Frontiers in Medicine, Год журнала: 2025, Номер 12

Опубликована: Март 25, 2025

Ulcerative colitis (UC) is a chronic idiopathic inflammatory disease affecting the colon and rectum. Characterized by recurrent attacks, UC often resistant to traditional anti-inflammatory therapies, imposing significant physiological, psychological, economic burdens on patients. In light of these challenges, innovative targeted therapies have become new expectation for patients with UC. A crucial pathological feature impairment intestinal mucosal barrier, which underlies aberrant immune responses inflammation. Intestinal stem cells (ISCs), differentiate into epithelial cells, play central role in maintaining this barrier. Growing studies proved that regulating regeneration differentiation ISC promising approach treating Despite progress, there dearth comprehensive articles describing ISCs This review focuses importance barrier discusses latest findings functions, markers, their regulatory mechanisms. Key pathways involved regulation, including Wnt, Notch, Hedgehog (HH), Hippo/Yap, autophagy pathways, are explored detail. Additionally, examines recent advances ISC-targeted UC, such as natural or synthetic compounds, microbial preparations, Chinese medicine (TCM) extracts transplantation therapy. aims offer novel therapeutic insights strategies who long struggled

Язык: Английский

Процитировано

0
Zinc pyrithione ameliorates colitis in mice by interacting on intestinal epithelial TRPA1 and TRPV4 channels DOI
Mengting Zhang,

Xiongying Chen,

Luyun Zhang

и другие.

Life Sciences, Год журнала: 2024, Номер 358, С. 123090 - 123090

Опубликована: Окт. 9, 2024

Язык: Английский

Процитировано

2
cGAS-STING DNA-sensing in inflammatory bowel diseases DOI

Georges Dimitrov,

Bernhard Ryffel, Dieudonnée Togbe

и другие.

Trends in Molecular Medicine, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

2
STING Drives Pyroptosis and Exacerbates Intestinal Ischemia-Reperfusion Injury DOI Creative Commons
Ke Ding, Yiguo Zhang, Lele Zhang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 15, 2024

Abstract This study aimed to investigate the role of STING (Stimulator Interferon Genes) in intestinal ischemia-reperfusion (IIR) injury, focusing on its impact mitochondrial function, pyroptosis, and inflammation. The utilized both vivo vitro models, including a mouse model IIR organoids subjected hypoxia-reoxygenation (HR). We assessed effects pharmacological inhibition using H.151 knockout injury markers, histological damage, pro-inflammatory cytokine levels, morphology pyroptosis markers. Our findings revealed that significantly upregulated expression epithelium, coinciding with increased permeability, elevated production (IL-1β) (p < 0.05), evidence dysfunction, characterized by reduced ATP disrupted 0.01). Importantly, treatment attenuated IIR-induced damage 0.01), levels preserved function Furthermore, we observed significant reduction as evidenced decreased cleaved gasdermin D H.151-treated groups 0.05). identifies critical mediator likely through activation pathways leading dysfunction pyroptosis. suggest targeting represents promising therapeutic strategy for mitigating following ischemia-reperfusion.

Язык: Английский

Процитировано

0