A Strong Acid‐Induced DNA Hydrogel Based on pH‐Reconfigurable A‐Motif Duplex DOI
Yuwei Hu, Jackie Y. Ying

Small, Год журнала: 2023, Номер 19(12)

Опубликована: Янв. 1, 2023

Abstract Under a pH value lower than the p K of adenine (3.5), adenine‐rich sequences (A‐strand) form unique parallel A‐motif duplex due to protonation A‐strand. At above 3.5, deprotonation adenines leads dissolution A‐strand single coil. This pH‐reconfigurable has been developed as novel pH‐responsive DNA hydrogel, termed A‐hydrogel. The hydrogel state is achieved at 1.2 by bridging units, which are cross‐linked both reverse Hoogsteen interaction and electrostatic attraction. Hydrogel‐to‐solution transition triggered 4.3 deprotonation‐induced separation duplex. A‐hydrogel system undergoes reversible hydrogel–solution transitions subjecting cyclic shifts between 4.3. An anti‐inflammatory medicine, sulfasalazine (SSZ), intercalates into duplex, loaded Its pH‐controlled release from successfully demonstrated. strong acid‐induced may fill gap that other mild acid‐responsive hydrogels cannot do, such protection orally delivered drug in hostile stomach environment against acid (pH ~ 1.2) digestive enzymes.

Язык: Английский

The Evolution of Nucleic Acid Nanotechnology: From DNA Assembly to DNA‐Encoded Library DOI Open Access
Qigui Nie, Xianfu Fang,

Jiale Huang

и другие.

Small Methods, Год журнала: 2025, Номер unknown

Опубликована: Янв. 13, 2025

Abstract Deoxyribonucleic acid (DNA), a fundamental biomacromolecule in living organisms, serves as the carrier of genetic information. Beyond its role encoding biological functions, DNA's inherent ability to hybridize through base pairing has opened new avenues for application sciences. This review introduces DNA nanotechnology and DNA‐encoded library (DEL), highlights their shared design principles related assembly. First, foundational overview structural nanotechnology, including strategies historical development is provided. Subsequently, various approaches are examined dynamic from strand displacement reactions DNA‐templated polymer synthesis. Second, how principle assembly facilitated diverse formats self‐assembly‐based DEL synthesis, DNA‐template (DTS), template‐mediated proximity induction effects examined. These advancements all underpinned by unique property Finally, this summarizes common terms methodology design. Additionally, potential synergies explored between these two technologies, envisioning future applications where they can be combined create more versatile exquisite functionalities.

Язык: Английский

Процитировано

1
Emerging Trends in DNA Nanotechnology-Enabled Cell Surface Engineering DOI Creative Commons
Fan Xiao,

Xuechu Shen,

Wenqi Tang

и другие.

JACS Au, Год журнала: 2025, Номер 5(2), С. 550 - 570

Опубликована: Фев. 6, 2025

Cell surface engineering is a rapidly advancing field, pivotal for understanding cellular physiology and driving innovations in biomedical applications. In this regard, DNA nanotechnology offers unprecedented potential precisely manipulating functionalizing cell surfaces by virtue of its inherent programmability versatile functionalities. Herein, Perspective provides comprehensive overview emerging trends engineering, focusing on key nanostructure-based tools, their roles regulating physiological processes, We first discuss the strategies integrating molecules onto surfaces, including attachment oligonucleotides higher-order nanostructure. Second, we summarize impact DNA-based various such as membrane protein degradation, signaling transduction, intercellular communication, construction artificial components. Third, highlight applications DNA-engineered targeted therapies cancer inflammation, well capture/protection diagnostic detection. Finally, address challenges future directions nanotechnology-based engineering. This aims to provide valuable insights rational design contributing development precise personalized medicine.

Язык: Английский

Процитировано

1
Pollen‐Based Magnetic Microrobots are Mediated by Electrostatic Forces to Attract, Manipulate, and Kill Cancer Cells DOI
Carmen C. Mayorga‐Martinez, Michaela Fojtů, Jan Vyskočil

и другие.

Advanced Functional Materials, Год журнала: 2022, Номер 32(46)

Опубликована: Сен. 2, 2022

Abstract Naturally occurring micro/nanoparticles provide an incredible array of potential sources when preparing hybrid micro/nanorobots and their intrinsic properties can be exploited as multitasking functionalities modern robotics well ensuring mass production availability. Herein, magnetic biological bots (BioBots) prepared from defatted sunflower pollen microparticles by ferromagnetic metal layer evaporation on one side its surface are described. It is demonstrated that the methodology employed introduces to microparticles‐based BioBots enable actuation. Interestingly, as‐prepared pollen‐based naturally attract cancer cells due opposite charges (positive negative, respectively). Such attracted then transported microrobots. This strong attraction also allows delivery drugs intended kill cells. Sunflower‐based fabricated in large quantities, programmable, making them promising candidates for cell therapy.

Язык: Английский

Процитировано

35
MicroRNA-122-functionalized DNA tetrahedron stimulate hepatic differentiation of human mesenchymal stem cells for acute liver failure therapy DOI Creative Commons
Hongyan Wei,

Fenfang Li,

Tiantian Xue

и другие.

Bioactive Materials, Год журнала: 2023, Номер 28, С. 50 - 60

Опубликована: Май 12, 2023

As the most abundant liver-specific microRNA, microRNA-122 (miR122) played a crucial role in differentiation of stem cells into hepatocytes. However, highly efficient miR122 delivery still confronts challenges including poor cellular uptake and easy biodegradation. Herein, we for first time demonstrated that tetrahedral DNA (TDN) nanoplatform had great potential inducing human mesenchymal (hMSCs) functional hepatocyte-like (HLCs) by transferring to hMSCs efficiently without any extrinsic factors. compared with miR122, miR122-functionalized TDN (TDN-miR122) could significantly up-regulate protein expression levels mature hepatocyte markers hepatocyte-specific marker genes hMSCs, indicating TDN-miR122 particularly activate properties developing cell-based therapies vitro. The transcriptomic analysis further indicated mechanism assisted differentiated HLCs. TDN-miR122-hMSCs exhibited hepatic cell morphology phenotype, up-regulated specific biofunctions comparison undifferentiated MSCs. Preclinical vivo transplantation appeared combination or rescue acute liver failure injury through function supplement, anti-apoptosis, proliferation promotion, anti-inflammatory. Collectively, our findings may provide new facile approach therapy. Further large animal model explorations are needed study their clinical translation future.

Язык: Английский

Процитировано

19
A Strong Acid‐Induced DNA Hydrogel Based on pH‐Reconfigurable A‐Motif Duplex DOI
Yuwei Hu, Jackie Y. Ying

Small, Год журнала: 2023, Номер 19(12)

Опубликована: Янв. 1, 2023

Abstract Under a pH value lower than the p K of adenine (3.5), adenine‐rich sequences (A‐strand) form unique parallel A‐motif duplex due to protonation A‐strand. At above 3.5, deprotonation adenines leads dissolution A‐strand single coil. This pH‐reconfigurable has been developed as novel pH‐responsive DNA hydrogel, termed A‐hydrogel. The hydrogel state is achieved at 1.2 by bridging units, which are cross‐linked both reverse Hoogsteen interaction and electrostatic attraction. Hydrogel‐to‐solution transition triggered 4.3 deprotonation‐induced separation duplex. A‐hydrogel system undergoes reversible hydrogel–solution transitions subjecting cyclic shifts between 4.3. An anti‐inflammatory medicine, sulfasalazine (SSZ), intercalates into duplex, loaded Its pH‐controlled release from successfully demonstrated. strong acid‐induced may fill gap that other mild acid‐responsive hydrogels cannot do, such protection orally delivered drug in hostile stomach environment against acid (pH ~ 1.2) digestive enzymes.

Язык: Английский

Процитировано

18