Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 24, 2024
Abstract
MicroRNA
(miRNA)‐based
therapy
holds
significant
potential;
however,
its
structural
limitations
pose
a
challenge
to
the
full
exploitation
of
biomedical
functionality.
Framework
nucleic
acids
are
promising
owing
their
transportability,
biocompatibility,
and
functional
editability.
MiRNA‐125
is
embedded
into
acid
framework
create
an
enzyme‐responsive
nanoparachute
(NP),
enhancing
miRNA
loading
capacity
while
preserving
attributes
small‐scale
circumventing
uncertainty
related
RNA
exposure
in
conventional
methods.
An
enzyme‐sensitive
sequence
designed
NP
as
bioswitchable
apparatus
for
cargo
miRNAs
release.
compared
with
delivery
modes
vehicles,
confirming
excellent
transportability
sustained
release
properties.
Moreover,
confers
good
enzyme
serum
resistance
miRNAs.
Simultaneously,
it
can
easily
deliver
miRNA‐125
liver
kidney
lesions
passive
targeting
This
allows
Keap1/Nrf2
pathway
regulation
p53
protein
affected
tissues.
Additionally,
negatively
regulates
expression
Bax
Caspase‐3.
These
combined
actions
help
inhibit
oxidation,
prevent
cell
cycle
arrest,
reduce
apoptosis
cells.
Consequently,
this
strategy
offers
potential
treatment
acute
injury.
Abstract
DNA
nanostructures
exhibit
versatile
geometries
and
possess
sophisticated
capabilities
not
found
in
other
nanomaterials.
They
serve
as
customizable
nanoplatforms
for
orchestrating
the
spatial
arrangement
of
molecular
components,
such
biomolecules,
antibodies,
or
synthetic
This
is
achieved
by
incorporating
oligonucleotides
into
design
nanostructure.
In
realm
drug
delivery
to
cancer
cells,
there
a
growing
interest
active
targeting
assays
enhance
efficacy
selectivity.
The
approach
involves
“key‐lock”
mechanism
where
carrier,
through
its
ligand,
recognizes
specific
receptors
on
tumor
facilitating
release
drugs.
Various
nanostructures,
including
origami,
Tetrahedral,
nanoflower,
cruciform,
nanostar,
nanocentipede,
nanococklebur,
can
traverse
lipid
layer
cell
membrane,
allowing
cargo
nucleus.
Aptamers,
easily
formed
vitro,
are
recognized
their
targeted
due
high
selectivity
targets
low
immunogenicity.
review
provides
comprehensive
overview
recent
advancements
formation
modification
aptamer‐modified
within
systems.
Journal of Inflammation Research,
Год журнала:
2024,
Номер
Volume 17, С. 171 - 189
Опубликована: Янв. 1, 2024
Abstract:
Chronic
liver
conditions
are
associated
with
high
mortality
rates
and
have
a
large
adverse
effect
on
human
well-being
as
well
significant
financial
burden.
Currently,
the
only
effective
treatment
available
for
effects
of
failure
cirrhosis
resulting
from
progression
several
chronic
diseases
is
transplantation
carried
out
at
original
location.
This
implies
that
developing
novel
treatments
imperative.
Regenerative
medicine
has
long
been
stem
cell
therapy.
Mesenchymal
cells
(MSCs),
type
great
differentiation
potential,
become
preferred
source
According
to
recent
studies,
MSCs'
paracrine
products—rather
than
their
capacity
differentiation—play
therapeutic
effect.
MSC
exosomes,
extracellular
vesicle
(MSC-EV),
came
into
view
substances
MSCs.
research,
exosomes
can
maintain
tissue
homeostasis,
which
necessary
healthy
function.
All
tissues
contain
them,
they
take
part
in
variety
biological
activities
support
cellular
activity
regeneration
order
preserve
homeostasis.
The
outcomes
use
MSCs
produce
option
range
diseases.
review
provides
brief
overview
MSC-EVs
outlines
physiological
roles
biochemical
capabilities.
elucidation
role
recovery
repair
hepatic
tissues,
contribution
maintaining
discussed
relation
different
aims
provide
new
insights
unique
play
Keywords:
mesenchymal
cells,
disease,
immunomodulation,
homeostasis
The
emerging
stem
cell-derived
hepatocyte-like
cells
(HLCs)
are
the
alternative
cell
sources
of
hepatocytes
for
treatment
highly
lethal
acute
liver
failure
(ALF).
However,
hostile
local
environment
and
immature
differentiation
may
compromise
their
therapeutic
efficacy.
To
this
end,
human
adipose-derived
mesenchymal
stromal/stem
(hASCs)
engineered
into
different-sized
multicellular
spheroids
co-cultured
with
3D
coaxially
hexagonally
patterned
umbilical
vein
endothelial
(HUVECs)
in
a
lobule-like
manner
to
enhance
hepatic
efficiency.
It
is
found
that
small-sized
hASC
spheroids,
diameter
≈50
µm,
show
superior
pro-angiogenic
effects
compared
other
counterparts.
size-dependent
functional
enhancements
mediated
by
Wnt
signaling
pathway.
Meanwhile,
co-culture
hASCs
HUVECs,
at
HUVECs/hASCs
seeding
density
ratio
2:1,
distinctly
promotes
vascularization
both
vitro
vivo,
especially
when
hollow
hexagons.
After
subcutaneous
implantation,
mini-liver,
consisting
HLC
3D-printed
interconnected
vasculatures,
can
effectively
improve
regeneration
two
ALF
animal
models
through
amelioration
oxidative
stress
inflammation,
reduction
necrosis,
as
well
increase
proliferation,
thereby
showing
great
promise
clinical
translation.
Advanced Materials,
Год журнала:
2023,
Номер
36(30)
Опубликована: Окт. 6, 2023
Abstract
Acute
liver
failure
(ALF)
is
a
life‐threatening
disease
associated
with
the
rapid
development
of
inflammatory
storms,
level
elevation
reactive
oxygen
species
(ROS),
and
hepatocyte
necrosis,
which
results
in
high
short‐term
mortality.
Except
for
transplantation,
no
effective
strategies
are
available
ALF
therapy
due
to
progression
narrow
window
therapeutic
time.
Therefore,
there
an
urgent
demand
explore
fast
modalities
treatment.
Herein,
multifunctional
tetrahedral
DNA
nanoplatform
(TDN)
constructed
by
incorporating
tumor
necrosis
factor‐α
siRNA
(siTNF‐α)
through
hybridization
antioxidant
manganese
porphyrin
(MnP4)
via
π–π
stacking
interaction
G‐quadruplex
(G4)
surprisingly
significant
therapy.
TDN‐siTNF‐α/‐G4‐MnP4
silences
TNF‐α
macrophages
siTNF‐α
polarizes
them
anti‐inflammatory
M2
phenotype,
providing
appropriate
microenvironments
viability.
Additionally,
scavenges
intracellular
ROS
MnP4,
protecting
hepatocytes
from
oxidative‐stress‐associated
cell
death.
Furthermore,
TDN
itself
promotes
proliferation
modulating
cycle.
shows
almost
complete
accumulation
after
intravenous
injection
exhibits
excellent
efficacy
within
2
h.
The
nanoformulation
provides
strategy
therapy,
expanding
its
application
innovative
treatments
diseases.
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(38)
Опубликована: Апрель 18, 2024
Abstract
Acute
liver
failure
(ALF)
involves
extensive
necrosis
of
cells
and
severe
impairment
function.
Hepatocyte
transplantation
holds
promise
for
treating
ALF
by
swiftly
supporting
functions
promoting
regeneration.
However,
the
scarcity
suitable
cell
sources
requires
strategies
to
obtain
enough
functional
hepatocyte‐like
(HLCs)
optimize
their
in
vivo
efficiency.
A
DNA
hexahedral
nanostructure
(DHN)
is
developed
loaded
with
microRNA‐122
efficiently
induce
hepatic
differentiation
human
adipose‐derived
mesenchymal
stem
into
HLCs.
These
HLCs
can
serve
as
alternative
hepatocyte
sources,
confirmed
expression
liver‐specific
genes
proteins,
restoration
functions.
To
enhance
survival
efficiency
HLCs,
a
versatile
scaffold
also
created
3D
printing
calcium‐cross‐linked
mixture
bioink
composed
sodium
alginate,
gelatin,
silk
fibroin
excellent
ROS
scavenging
capabilities.
The
infused
chitosan‐DHN
hydrogel
containing
tissue
engineering
orthotopic
CCl
4
‐induced
mice.
transplanted
composite
scaffold‐HLCs
successfully
repair
injury
area
mice
regulate
expressions
proteins
associated
inflammation,
oxidative
stress,
Collectively,
this
study
offers
novel
approach
strategy
identifying
ALF.
Abstract
Liver
fibrosis
is
a
chronic
pathological
condition
lacking
specific
clinical
treatments.
Stem
cells,
with
notable
potential
in
regenerative
medicine,
offer
promise
treating
liver
fibrosis.
However,
stem
cell
therapy
hindered
by
immunological
rejection,
carcinogenesis
risk,
efficacy
variation,
and
high
cost.
secretome‐based
cell‐free
offers
solutions
to
address
these
challenges,
but
it
limited
low
delivery
efficiency
rapid
clearance.
Herein,
an
innovative
approach
for
situ
implantation
of
smart
microneedle
(MN)
arrays
enabling
precisely
controlled
multiple
therapeutic
agents
directly
into
fibrotic
tissues
developed.
By
integrating
platinum‐based
nanocatalytic
combination
therapy,
the
MN
can
deactivate
hepatic
stellate
cells.
Moreover,
they
promote
excessive
extracellular
matrix
degradation
more
than
75%,
approaching
normal
levels.
Additionally,
provide
hepatocyte
protection
while
reducing
inflammation
levels
≈70–90%.
They
also
exhibit
remarkable
capability
scavenging
almost
100%
reactive
oxygen
species
alleviating
hypoxia.
Ultimately,
this
treatment
strategy
effectively
restrain
progression.
The
comprehensive
vitro
vivo
experiments,
supplemented
proteome
transcriptome
analyses,
substantiate
effectiveness
fibrosis,
holding
immense
applications.
Tissue Engineering Part B Reviews,
Год журнала:
2024,
Номер
30(6), С. 607 - 630
Опубликована: Март 14, 2024
Regenerative
medicine
aims
to
restore
the
function
of
diseased
or
damaged
tissues
and
organs
by
cell
therapy,
gene
tissue
engineering,
along
with
adjunctive
application
bioactive
molecules.
Traditional
molecules
such
as
growth
factors
cytokines
have
shown
great
potential
in
regulation
cellular
behavior,
but
disadvantages
limited
source,
high
cost,
short
half-life,
side
effects.
In
recent
years,
herbal
compounds
extracted
from
natural
plants/herbs
gained
increasing
attention.
This
is
not
only
because
are
easily
obtained,
inexpensive,
mostly
safe,
reliable,
also
owing
their
excellent
effects
including
anti-inflammatory,
antibacterial,
antioxidative,
proangiogenic
behavior
ability
promote
stem
differentiation.
Such
play
important
roles
processes
related
regeneration.
Furthermore,
moieties
can
form
physical
chemical
bonds
scaffolds,
which
contributes
improved
mechanical
strength
stability
scaffolds.
Thus,
incorporation
biomaterials
a
promising
direction
for
future
regenerative
applications.
Herein,
an
overview
on
use
combined
different
biomaterial
scaffolds
presented.
We
firstly
introduce
classification,
structures,
properties
components
then
provide
comprehensive
survey
engineer
repair/regeneration
skin,
cartilage,
bone,
neural,
heart
tissues.
Finally,
we
highlight
challenges
prospects
development
toward
clinical
translation.
Overall,
it
believed
that
combination
could
be
perspective
next
generation
medicine.
