Phage therapy: From biological mechanisms to future directions

Cell, Год журнала: 2023, Номер 186(1), С. 17 - 31

Опубликована: Янв. 1, 2023

Increasing antimicrobial resistance rates have revitalized bacteriophage (phage) research, the natural predators of bacteria discovered over 100 years ago. In order to use phages therapeutically, they should (1) preferably be lytic, (2) kill bacterial host efficiently, and (3) fully characterized exclude side effects. Developing therapeutic takes a coordinated effort multiple stakeholders. Herein, we review state art in phage therapy, covering biological mechanisms, clinical applications, remaining challenges, future directions involving naturally occurring genetically modified or synthetic phages.

Язык: Английский

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Microbially mediated metal corrosion

Nature Reviews Microbiology, Год журнала: 2023, Номер 21(11), С. 705 - 718

Опубликована: Июнь 21, 2023

Язык: Английский

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Microbiome and Human Health: Current Understanding, Engineering, and Enabling Technologies

Chemical Reviews, Год журнала: 2022, Номер 123(1), С. 31 - 72

Опубликована: Ноя. 1, 2022

The human microbiome is composed of a collection dynamic microbial communities that inhabit various anatomical locations in the body. Accordingly, coevolution with host has resulted these playing profound role promoting health. Consequently, perturbations can cause or exacerbate several diseases. In this Review, we present our current understanding relationship between health and disease development, focusing on microbiomes found across digestive, respiratory, urinary, reproductive systems as well skin. We further discuss strategies by which composition function be modulated to exert therapeutic effect host. Finally, examine technologies such multiomics approaches cellular reprogramming microbes enable significant advancements research engineering.

Язык: Английский

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Microbiota succession throughout life from the cradle to the grave

Nature Reviews Microbiology, Год журнала: 2022, Номер 20(12), С. 707 - 720

Опубликована: Июль 29, 2022

Язык: Английский

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CRISPR-based genome editing through the lens of DNA repair

Molecular Cell, Год журнала: 2022, Номер 82(2), С. 348 - 388

Опубликована: Янв. 1, 2022

Язык: Английский

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Opportunities and challenges of using metagenomic data to bring uncultured microbes into cultivation

Microbiome, Год журнала: 2022, Номер 10(1)

Опубликована: Май 11, 2022

Although there is now an extensive understanding of the diversity microbial life on earth through culture-independent metagenomic DNA sequence analyses, isolation and cultivation microbes remains critical to directly study them confirm their metabolic physiological functions, ecological roles. The majority environmental are as yet uncultured however; therefore, bringing these rare or poorly characterized groups into culture a priority further understand microbiome functions. Moreover, cultivated isolates may find utility in range applications, such new probiotics, biocontrol agents, agents for industrial processes. growing abundance meta-transcriptomic information from wide environments provides more opportunities guide interest. In this paper, we discuss successful methodologies applications that have underpinned recent metagenome-guided microbe efforts. These approaches include determining specific conditions enrich taxa interest, complex strategies specifically target capture species antibody engineering genome editing strategies. With greater degree genomic available uncultivated members, via metagenome-assembled genomes, theoretical requirements will enable possibilities ultimately gain comprehensive microbiomes. Video Abstract.

Язык: Английский

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Structural biology of CRISPR–Cas immunity and genome editing enzymes

Nature Reviews Microbiology, Год журнала: 2022, Номер 20(11), С. 641 - 656

Опубликована: Май 13, 2022

Язык: Английский

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The Role of Synthetic Microbial Communities (SynCom) in Sustainable Agriculture

Frontiers in Agronomy, Год журнала: 2022, Номер 4

Опубликована: Июнь 30, 2022

Modern agriculture faces several challenges due to climate change, limited resources, and land degradation. Plant-associated soil microbes harbor beneficial plant growth-promoting (PGP) traits that can be used address some of these challenges. These are often formulated as inoculants for many crops. However, inconsistent productivity a problem since the performance individual inoculants/microbes vary with environmental conditions. Over past decade, ability utilize Next Generation Sequencing (NGS) approaches has led an explosion information regarding associated microbiomes. Although this type work been predominantly sequence-based descriptive in nature, increasingly it is moving towards microbiome functionality. The synthetic microbial communities (SynCom) approach emerging technique involves co-culturing multiple taxa under well-defined conditions mimic structure function microbiome. SynCom hopes increase community stability through synergistic interactions between its members. This review will focus on plant-soil-microbiome how they have potential improve crop production. Current formulation discussed, practical application considered.

Язык: Английский

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Soil microbiome engineering for sustainability in a changing environment

Nature Biotechnology, Год журнала: 2023, Номер 41(12), С. 1716 - 1728

Опубликована: Окт. 30, 2023

Язык: Английский

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Intestinal transgene delivery with native E. coli chassis allows persistent physiological changes

Cell, Год журнала: 2022, Номер 185(17), С. 3263 - 3277.e15

Опубликована: Авг. 1, 2022

Live bacterial therapeutics (LBTs) could reverse diseases by engrafting in the gut and providing persistent beneficial functions host. However, attempts to functionally manipulate microbiome of conventionally raised (CR) hosts have been unsuccessful because engineered microbial organisms (i.e., chassis) difficulty colonizing hostile luminal environment. In this proof-of-concept study, we use native bacteria as chassis for transgene delivery impact CR host physiology. Native Escherichia coli isolated from stool cultures mice were modified express functional genes. The reintroduction these strains induces perpetual engraftment intestine. addition, E. can induce changes that affect physiology pathology months after administration. Thus, using "knock in" specific allows mechanistic studies activities enables LBT with curative intent.

Язык: Английский

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