CRISPR technologies for genome, epigenome and transcriptome editing

Nature Reviews Molecular Cell Biology, Год журнала: 2024, Номер 25(6), С. 464 - 487

Опубликована: Фев. 2, 2024

Язык: Английский

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Precise cut-and-paste DNA insertion using engineered type V-K CRISPR-associated transposases

Nature Biotechnology, Год журнала: 2023, Номер 41(7), С. 968 - 979

Опубликована: Янв. 2, 2023

Язык: Английский

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CRISPR-Cas-Based Antimicrobials: Design, Challenges, and Bacterial Mechanisms of Resistance

ACS Infectious Diseases, Год журнала: 2023, Номер 9(7), С. 1283 - 1302

Опубликована: Июнь 22, 2023

The emergence of antibiotic-resistant bacterial strains is a source public health concern across the globe. As discovery new conventional antibiotics has stalled significantly over past decade, there an urgency to develop novel approaches address drug resistance in infectious diseases. use CRISPR-Cas-based system for precise elimination targeted populations holds promise as innovative approach antimicrobial agent design. CRISPR-Cas targeting celebrated its high versatility and specificity, offering excellent opportunity fight antibiotic pathogens by selectively inactivating genes involved resistance, biofilm formation, pathogenicity, virulence, or viability. strategy can enact effects two approaches: inactivation chromosomal curing plasmids encoding resistance. In this Review, we provide overview main systems utilized creation these antimicrobials, well highlighting promising studies field. We also offer detailed discussion about most commonly used mechanisms delivery: bacteriophages, nanoparticles, conjugative plasmids. Lastly, possible interference that should be considered during intelligent design approaches.

Язык: Английский

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Experimental warming accelerates positive soil priming in a temperate grassland ecosystem

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Фев. 8, 2024

Abstract Unravelling biosphere feedback mechanisms is crucial for predicting the impacts of global warming. Soil priming, an effect fresh plant-derived carbon (C) on native soil organic (SOC) decomposition, a key mechanism that could release large amounts C into atmosphere. However, climate warming priming remain elusive. Here, we show experimental accelerates by 12.7% in temperate grassland. Warming alters bacterial communities, with 38% unique active phylotypes detected under The functional genes essential decomposition are also stimulated, which be linked to effects. We incorporate lab-derived information ecosystem model showing parameter uncertainty can reduced 32–37%. Model simulations from 2010 2016 indicate increase warming, 9.1% rise priming-induced CO 2 emissions. If our findings generalized other ecosystems over extended period time, play important role terrestrial cycle feedbacks and change.

Язык: Английский

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Recent advances in CRISPR-Cas9-based genome insertion technologies

Molecular Therapy — Nucleic Acids, Год журнала: 2024, Номер 35(1), С. 102138 - 102138

Опубликована: Фев. 5, 2024

Programmable genome insertion (or knock-in) is vital for both fundamental and translational research. The continuously expanding number of CRISPR-based strategies demonstrates the ongoing development in this field. Common methods site-specific rely on cellular double-strand breaks repair pathways, such as homology-directed repair, non-homologous end-joining, microhomology-mediated end joining. Recent advancements have further expanded toolbox programmable techniques, including prime editing, integrase coupled with nuclease, CRISPR-associated transposon. These tools possess their own capabilities limitations, promoting tremendous efforts to enhance editing efficiency, broaden targeting scope improve specificity. In review, we first summarize recent advances techniques. We then elaborate cons pros each technique assist researchers making informed choices when using these tools. Finally, identify opportunities future improvements applications basic research therapeutics.

Язык: Английский

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Precise genome-editing in human diseases: mechanisms, strategies and applications

Signal Transduction and Targeted Therapy, Год журнала: 2024, Номер 9(1)

Опубликована: Фев. 26, 2024

Precise genome-editing platforms are versatile tools for generating specific, site-directed DNA insertions, deletions, and substitutions. The continuous enhancement of these has led to a revolution in the life sciences, which promises deliver novel therapies genetic disease. can be traced back 1950s with discovery DNA's double-helix and, after 70 years development, evolved from crude vitro applications wide range sophisticated capabilities, including vivo applications. Nonetheless, precise faces constraints such as modest efficiency, delivery challenges, off-target effects. In this review, we explore genome-editing, focus on introduction landmark events its history, various platforms, systems, First, discuss history genome-editing. Second, describe current state strategies explain how techniques offer unprecedented precision versatility modifying human genome. Third, introduce systems used deploy components through DNA, RNA, RNPs. Finally, summarize labeling endogenous genes, screening variants, molecular recording, disease models, gene therapy, ex therapy potential future advances.

Язык: Английский

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Bacterial genome engineering using CRISPR-associated transposases

Nature Protocols, Год журнала: 2024, Номер 19(3), С. 752 - 790

Опубликована: Янв. 12, 2024

Язык: Английский

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Choreographing root architecture and rhizosphere interactions through synthetic biology

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Фев. 14, 2024

Abstract Climate change is driving extreme changes to the environment, posing substantial threats global food security and bioenergy. Given direct role of plant roots in mediating plant-environment interactions, engineering form function root systems their associated microbiota may mitigate these effects. Synthetic genetic circuits have enabled sophisticated control gene expression microbial for years a surge advances has heralded extension this approach multicellular species. Targeting tools affect structure, exudation, microbe activity on surfaces provide multiple strategies advancement climate-ready crops.

Язык: Английский

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Diverse anti-defence systems are encoded in the leading region of plasmids

Nature, Год журнала: 2024, Номер 635(8037), С. 186 - 192

Опубликована: Окт. 9, 2024

Plasmids are major drivers of gene mobilization by means horizontal transfer and play a key role in spreading antimicrobial resistance among pathogens

Язык: Английский

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Exploring the plant microbiome: A pathway to climate-smart crops

Cell, Год журнала: 2025, Номер 188(6), С. 1469 - 1485

Опубликована: Март 1, 2025

Язык: Английский

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