ACS Nano,
Год журнала:
2023,
Номер
17(12), С. 11466 - 11480
Опубликована: Май 18, 2023
Intratumoral
pathogens
can
contribute
to
cancer
progression
and
affect
therapeutic
response.
Fusobacterium
nucleatum,
a
core
pathogen
of
colorectal
(CRC),
is
an
important
cause
low
efficacy
metastasis.
Thus,
the
modulation
intratumoral
may
provide
target
for
therapy
metastasis
inhibition.
Herein,
we
propose
F.
nucleatum-modulating
strategy
enhancing
CRC
inhibiting
lung
by
designing
antibacterial
nanoplatform
(Au@BSA-CuPpIX),
which
produced
reactive
oxygen
species
(ROS)
under
ultrasound
exhibited
strong
activity.
Importantly,
Au@BSA-CuPpIX
reduced
levels
apoptosis-inhibiting
proteins
thereby
ROS-induced
apoptosis.
In
vivo
results
demonstrated
that
effectively
eliminated
nucleatum
enhance
sonodynamic
(SDT)
orthotopic
inhibit
Notably,
entrapped
gold
nanoparticles
phototoxicity
metalloporphyrin
accumulated
in
skin
during
tumor
treatment,
preventing
severe
inflammation
damage
skin.
Therefore,
this
study
proposes
elimination
effect
SDT,
thus
providing
promising
paradigm
improving
treatment
with
fewer
toxic
side
effects
promoting
clinical
translational
potential
SDT.
Journal of Experimental & Clinical Cancer Research,
Год журнала:
2023,
Номер
42(1)
Опубликована: Янв. 10, 2023
Colorectal
cancer
(CRC)
is
the
third
most
common
in
world,
and
a
strong
relationship
exists
between
CRC
gut
microbiota,
which
affects
occurrence,
development,
metastasis
of
cancer.
Bioinformatics-based
analyses
revealed
that
abundance
Parvimonas
micra
(P.
micra)
feces
patients
with
significantly
higher
than
healthy
people.
Therefore,
an
important
may
exist
P.
CRC.We
first
confirmed
can
promote
proliferation
cell
lines
through
experiments
mouse
models.
Then
we
selected
signaling
pathways
content
exosomes
to
development
by
transcriptomics
microRNA
sequencing.
Finally,
promoted
miR-218-5p/Ras/ERK/c-Fos
pathway
vivo
vitro
experiments.First,
it
was
CRC.
Transcriptome
analysis
after
coincubation
bacteria
cells
activating
Ras/ERK/c-Fos
pathway.
Furthermore,
sequencing
showed
miR-218-5p
protein
tyrosine
phosphatase
receptor
R
(PTPRR)
were
key
factors
involved
pathway,
inhibitor
used
confirm
role
xenograft
mice.This
experiment
upregulating
expression
exosomes,
inhibiting
PTPRR
expression,
ultimately
Nature Medicine,
Год журнала:
2024,
Номер
30(5), С. 1339 - 1348
Опубликована: Апрель 30, 2024
Despite
substantial
progress
in
cancer
microbiome
research,
recognized
confounders
and
advances
absolute
quantification
remain
underused;
this
raises
concerns
regarding
potential
spurious
associations.
Here
we
study
the
fecal
microbiota
of
589
patients
at
different
colorectal
(CRC)
stages
compare
observations
with
up
to
15
published
studies
(4,439
controls
total).
Using
quantitative
profiling
based
on
16S
ribosomal
RNA
amplicon
sequencing,
combined
rigorous
confounder
control,
identified
transit
time,
calprotectin
(intestinal
inflammation)
body
mass
index
as
primary
microbial
covariates,
superseding
variance
explained
by
CRC
diagnostic
groups.
Well-established
targets,
such
Fusobacterium
nucleatum,
did
not
significantly
associate
groups
(healthy,
adenoma
carcinoma)
when
controlling
for
these
covariates.
In
contrast,
associations
Anaerococcus
vaginalis,
Dialister
pneumosintes,
Parvimonas
micra,
Peptostreptococcus
anaerobius,
Porphyromonas
asaccharolytica
Prevotella
intermedia
remained
robust,
highlighting
their
future
target
potential.
Finally,
control
individuals
(age
22-80
years,
mean
57.7
standard
deviation
11.3)
meeting
criteria
colonoscopy
(for
example,
through
a
positive
immunochemical
test)
but
without
colonic
lesions
are
enriched
dysbiotic
Bacteroides2
enterotype,
emphasizing
uncertainties
defining
healthy
research.
Together,
results
indicate
importance
covariate
biomarker
identification
studies.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Фев. 24, 2024
Fusobacterium
nucleatum
(F.
nucleatum)
promotes
intestinal
tumor
growth
and
its
relative
abundance
varies
greatly
among
patients
with
CRC,
suggesting
the
presence
of
unknown,
individual-specific
effectors
in
F.
nucleatum-dependent
carcinogenesis.
Here,
we
identify
that
is
enriched
preferentially
KRAS
p.G12D
mutant
CRC
tissues
contributes
to
colorectal
tumorigenesis
Villin-Cre/Kras
Microbial Genomics,
Год журнала:
2024,
Номер
10(2)
Опубликована: Фев. 29, 2024
Molecular
profiling
techniques
such
as
metagenomics,
metatranscriptomics
or
metabolomics
offer
important
insights
into
the
functional
diversity
of
microbiome.
In
contrast,
16S
rRNA
gene
sequencing,
a
widespread
and
cost-effective
technique
to
measure
microbial
diversity,
only
allows
for
indirect
estimation
function.
To
mitigate
this,
tools
PICRUSt2,
Tax4Fun2,
PanFP
MetGEM
infer
profiles
from
sequencing
data
using
different
algorithms.
Prior
studies
have
cast
doubts
on
quality
these
predictions,
motivating
us
systematically
evaluate
matched
metagenomic
datasets,
simulated
data.
Our
contribution
is
threefold:
(i)
data,
we
investigate
if
technical
biases
could
explain
discordance
between
inferred
expected
results;
(ii)
considering
human
cohorts
type
two
diabetes,
colorectal
cancer
obesity,
test
health-related
differential
abundance
measures
categories
are
concordant
gene-inferred
metagenome-derived
and;
(iii)
since
copy
number
an
confounder
in
inference,
customised
normalisation
with
rrnDB
database
improve
results.
results
show
that
gene-based
inference
generally
do
not
necessary
sensitivity
delineate
changes
microbiome
should
thus
be
used
care.
Furthermore,
outline
differences
individual
tested
recommendations
tool
selection.
The
polymorphic
microbiome
is
considered
a
new
hallmark
of
cancer.
Advances
in
High-Throughput
Sequencing
have
fostered
rapid
developments
research.
interaction
between
cancer
cells,
immune
and
microbiota
defined
as
the
immuno-oncology
(IOM)
axis.
Fungal
microbes
(the
mycobiome),
although
representing
only
∼
0.1-1%
microbiome,
are
critical
immunologically
active
component
tumor
microbiome.
Accumulating
evidence
suggests
possible
involvement
commensal
pathogenic
fungi
initiation,
progression,
treatment
responsiveness.
tumor-associated
mycobiome
mainly
consists
gut
mycobiome,
oral
intratumoral
mycobiome.
However,
role
remains
poorly
understood,
diversity
complexity
analytical
methods
make
it
challenging
to
access
this
field.
This
review
aims
elucidate
causal
complicit
roles
development
progression
while
highlighting
issues
that
need
be
addressed
executing
such
We
systematically
summarize
advantages
limitations
current
fungal
detection
analysis
methods.
enumerate
integrate
these
recent
findings
into
our
understanding
accompanied
by
prospect
novel
exhilarating
clinical
implications.
The Innovation,
Год журнала:
2025,
Номер
6(3), С. 100784 - 100784
Опубликована: Янв. 18, 2025
Aquatic
vegetation
(AV)
is
vital
for
maintaining
the
health
of
lake
ecosystems,
with
submerged
aquatic
(SAV)
and
floating/emergent
(FEAV)
representing
clear
shaded
states,
respectively.
However,
global
SAV
FEAV
dynamics
are
poorly
understood
due
to
data
scarcity.
To
address
this
gap,
we
developed
an
innovative
AV
mapping
algorithm
workflow
using
satellite
imagery
(1.4
million
Landsat
images)
from
1989
2021
created
a
database
across
5,587
shallow
lakes.
Our
findings
suggest
that
covers
108,186
km2
on
average
globally,
accounting
28.9%
(FEAV,
15.8%;
SAV,
13.1%)
total
area.
Over
two
decades,
observed
notable
transition:
decreased
by
30.4%,
while
increased
15.6%,
leading
substantial
net
loss
AV.
This
trend
indicates
shift
conditions,
increasingly
progressing
toward
turbid
states
dominated
phytoplankton.
We
found
human-induced
eutrophication
was
primary
driver
change
until
early
2010s,
after
which
warming
rising
temperatures
became
dominant
drivers.
These
trends
serve
as
warning
sign
deteriorating
worldwide.
With
future
climate
intensified
eutrophication,
these
ongoing
pose
significant
risk
disrupting
ecosystems.
