Overcoming donor variability and risks associated with fecal microbiota transplants through bacteriophage-mediated treatments DOI Creative Commons
Torben Sølbeck Rasmussen, Xiaotian Mao, Sarah Förster

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Март 17, 2023

ABSTRACT Background Fecal microbiota transplantation (FMT) and fecal virome (FVT, sterile filtrated donor feces) have been effective in treating recurrent Clostridioides difficile infections, possibly through bacteriophage-mediated modulation of the gut microbiome. However, challenges like variability, costly screening, coupled with concerns over pathogen transfer (incl. eukaryotic viruses) FMT or FVT hinders their wider clinical application less acute diseases. Methods To overcome these challenges, we developed methods to broaden FVT’s while maintaining efficacy increasing safety. Specifically, employed following approaches: 1) Chemostat-fermentation reproduce bacteriophage component remove viruses (FVT-ChP), 2) solvent-detergent treatment inactivate enveloped (FVT-SDT), 3) pyronin-Y inhibit RNA-virus replication (FVT-PyT). We assessed processed FVTs a C. infection mouse model compared them untreated (FVT-UnT), FMT, saline. Results FVT-SDT, FVT-UnT, FVT-ChP reduced incidence mice reaching humane endpoint (0/8, 2/7, 3/8, respectively) FVT-PyT, saline control (5/8, 7/8, 5/7, significantly load colonizing cells toxin A/B levels. There was potential elimination colonization, 7 out 8 treated FVT-SDT testing negative qPCR. In contrast, all other treatments exhibited continued presence . Moreover, results were supported by changes microbiome profiles, cecal cytokine levels histopathological findings. Assessment viral engraftment FMT/FVT host-phage correlations analysis suggested that phages likely an important contributing factor associated efficacy. Conclusions This proof-of-concept study show specific modifications hold promise addressing related variability risks. Two strategies lead limiting colonization mice, solvent/detergent chemostat-propagation emerging as promising approaches.

Язык: Английский

A gut (microbiome) feeling about addiction: Interactions with stress and social systems DOI Creative Commons
Rubén García‐Cabrerizo, John F. Cryan

Neurobiology of Stress, Год журнала: 2024, Номер 30, С. 100629 - 100629

Опубликована: Март 18, 2024

In recent years, an increasing attention has given to the intricate and diverse connection of microorganisms residing in our gut their impact on brain health central nervous system disease. There been a shift mindset understand that drug addiction is not merely condition affects brain, it now being recognized as disorder also involves external factors such intestinal microbiota, which could influence vulnerability development addictive behaviors. Furthermore, stress social interactions, are closely linked powerful modulators addiction. This review delves into mechanisms through microbiota-stress-immune axis may shape work integrates preclinical clinical evidence demonstrate bidirectional communication between stress, behaviors, substance use disorders suggesting microbes might modulate having significance

Язык: Английский

Процитировано

11
Overcoming donor variability and risks associated with fecal microbiota transplants through bacteriophage-mediated treatments DOI Creative Commons
Torben Sølbeck Rasmussen, Xiaotian Mao, Sarah Förster

и другие.

Microbiome, Год журнала: 2024, Номер 12(1)

Опубликована: Июль 1, 2024

Abstract Background Fecal microbiota transplantation (FMT) and fecal virome (FVT, sterile filtrated donor feces) have been effective in treating recurrent Clostridioides difficile infections, possibly through bacteriophage-mediated modulation of the gut microbiome. However, challenges like variability, costly screening, coupled with concerns over pathogen transfer (incl. eukaryotic viruses) FMT or FVT hinder their wider clinical application less acute diseases. Methods To overcome these challenges, we developed methods to broaden FVT’s while maintaining efficacy increasing safety. Specifically, employed following approaches: (1) chemostat-fermentation reproduce bacteriophage component remove viruses (FVT-ChP), (2) solvent-detergent treatment inactivate enveloped (FVT-SDT), (3) pyronin-Y inhibit RNA virus replication (FVT-PyT). We assessed processed FVTs a C. infection mouse model compared them untreated (FVT-UnT), FMT, saline. Results FVT-SDT, FVT-UnT, FVT-ChP reduced incidence mice reaching humane endpoint (0/8, 2/7, 3/8, respectively) FVT-PyT, saline (5/8, 7/8, 5/7, significantly load colonizing cells associated toxin A/B levels. There was potential elimination colonization, seven out eight treated FVT-SDT testing negative qPCR. In contrast, all other treatments exhibited continued presence . Moreover, results were supported by changes microbiome profiles, cecal cytokine levels, histopathological findings. Assessment viral engraftment FMT/FVT host-phage correlations analysis suggested that phages likely an important contributing factor efficacy. Conclusions This proof-of-concept study shows specific modifications hold promise addressing related variability risks. Two strategies lead limiting colonization mice, solvent/detergent chemostat propagation emerging as promising approaches.

Язык: Английский

Процитировано

10
I "Gut" Rhythm: the microbiota as a modulator of the stress response and circadian rhythms DOI Creative Commons
Gabriel S. S. Tofani, Gerard Clarke, John F. Cryan

и другие.

FEBS Journal, Год журнала: 2025, Номер unknown

Опубликована: Янв. 22, 2025

Modern habits are becoming more and disruptive to health. As our days often filled with circadian disruption stress exposures, we need understand how responses these external stimuli shaped their mediators can be targeted promote A growing body of research demonstrates the role gut microbiota in influencing brain function behavior. The response rhythms, which essential maintaining appropriate environment, known impacted by microbiota. Gut microbes have been shown alter host's modulate rhythmicity. Although studies demonstrated strong links between microbiota, rhythms response, such were conducted an independent manner not conducive understanding interface factors. Due interconnected nature this review explore may play a regulating integration signals mammals consequences for health disease.

Язык: Английский

Процитировано

1
The gut microbiota-immune-brain axis: Therapeutic implications DOI Creative Commons
Kenneth J. O’Riordan, Gerard M. Moloney, Lily Keane

и другие.

Cell Reports Medicine, Год журнала: 2025, Номер unknown, С. 101982 - 101982

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

1
Metagenomic symphony of the intestinal ecosystem: How the composition affects the mind DOI Creative Commons
Stefanie Malan‐Müller, David Martín‐Hernández, Javier R. Caso

и другие.

Brain Behavior and Immunity, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

5
A review of bacteriophage and their application in domestic animals in a post-antibiotic era DOI
Xinyu Guo,

Guowang Luo,

Fujiang Hou

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 949, С. 174931 - 174931

Опубликована: Июль 21, 2024

Язык: Английский

Процитировано

4
Sex specific gut-microbiota signatures of resilient and comorbid gut-brain phenotypes induced by early life stress DOI Creative Commons
Lars Wilmes, Valentina Caputi, Thomaz F. S. Bastiaanssen

и другие.

Neurobiology of Stress, Год журнала: 2024, Номер 33, С. 100686 - 100686

Опубликована: Ноя. 1, 2024

Alterations in gut-brain axis communication pathways and the gut microbiota ecosystem caused by early life stress have been extensively described as critical players pathophysiology of stress-induced disorders. However, extent to which alterations manifest contribute sex-specific susceptibility distinct phenotypes adulthood has yet be defined.

Язык: Английский

Процитировано

4
Benchmarking of a time-saving and scalable protocol for the extraction of DNA from diverse viromes DOI Creative Commons
Michael Shamash,

Saniya Kapoor,

Corinne F. Maurice

и другие.

PeerJ, Год журнала: 2025, Номер 13, С. e18785 - e18785

Опубликована: Янв. 14, 2025

The virome, composed of viruses inhabiting diverse ecosystems, significantly influences microbial community dynamics and host health. phenol-chloroform DNA extraction protocol for viromes, though effective, is time-intensive requires the use multiple toxic chemicals. This study introduces a streamlined, scalable using commercially-available kit as an alternative, assessing its performance against method across human fecal, mouse soil samples. No significant differences in virome diversity or composition were seen between methods. Most viral operational taxonomic units (vOTUs) common to both methods, with only small percentage unique either approach. Alpha- beta-diversity analyses showed no impact on composition, confirming kit’s efficacy versatility sample types beyond those officially supported by manufacturer. While approach offers benefits like reduced toxicity increased throughput, it has limitations such higher costs potential issues reliably capturing low-abundance taxa. provides viable option large-scale studies, although may still be preferable specific types.

Язык: Английский

Процитировано

0
Precision microbial regulation: Strategies for modulating GIT microbiota for host health DOI Creative Commons

Zhong Pei,

Qin Li, Yanmei Zhang

и другие.

iMetaOmics., Год журнала: 2025, Номер unknown

Опубликована: Янв. 14, 2025

Abstract Recent advancements in analytical techniques have unveiled the spatiotemporal diversity of gastrointestinal tract (GIT) microbiota and their associations with host well‐being. Despite these insights, precise regulation GIT remains a significant challenge. Currently, microbial regulatory strategies, including fecal transplantation (FMT), synthetic communities (SynComs), genetically engineered microorganisms (GEMs), phages, nanomaterials, are increasingly utilized for influence on microbiota. This review emphasizes necessity developing targeted strategies provides comprehensive summary comparison approaches to explore potential. We discuss recent focusing mechanisms, efficacy, safety considerations, clinical trials, optimization at application level. Finally, we highlight support methods optimizing modulation timing regulation, selection targets, importance monitoring environment guide effective interventions.

Язык: Английский

Процитировано

0
Gut phages and their interactions with bacterial and mammalian hosts DOI Creative Commons
Marshall Godsil, Nathaniel L. Ritz, Siddarth Venkatesh

и другие.

Journal of Bacteriology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 23, 2025

ABSTRACT The mammalian gut microbiome is a dense and diverse community of microorganisms that reside in the distal gastrointestinal tract. In recent decades, bacterial members have been subject intense research. Less well studied large bacteriophages gut, which number billions viral particles per gram feces, consist considerable unknown “dark matter.” This gut-residing bacteriophages, called “phageome,” plays central role through predation transformation native bacteria, interactions with their hosts. this review, we will summarize what known about composition origins phageome, as its homeostasis host health. Furthermore, outline phages hosts, plot course for mechanistic study these systems.

Язык: Английский

Процитировано

0