A conceptual framework for understanding phase separation and addressing open questions and challenges

Molecular Cell, Год журнала: 2022, Номер 82(12), С. 2201 - 2214

Опубликована: Июнь 1, 2022

Язык: Английский

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Phase Transitions of Associative Biomacromolecules

Chemical Reviews, Год журнала: 2023, Номер 123(14), С. 8945 - 8987

Опубликована: Март 7, 2023

Multivalent proteins and nucleic acids, collectively referred to as multivalent associative biomacromolecules, provide the driving forces for formation compositional regulation of biomolecular condensates. Here, we review key concepts phase transitions aqueous solutions specifically that include folded domains intrinsically disordered regions. The these systems come under rubric coupled segregative transitions. underlying processes are presented, their relevance condensates is discussed.

Язык: Английский

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The Mediator complex as a master regulator of transcription by RNA polymerase II

Nature Reviews Molecular Cell Biology, Год журнала: 2022, Номер 23(11), С. 732 - 749

Опубликована: Июнь 20, 2022

Язык: Английский

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Capillary forces generated by biomolecular condensates

Nature, Год журнала: 2022, Номер 609(7926), С. 255 - 264

Опубликована: Сен. 7, 2022

Язык: Английский

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Transcription activation is enhanced by multivalent interactions independent of phase separation

Molecular Cell, Год журнала: 2022, Номер 82(10), С. 1878 - 1893.e10

Опубликована: Май 1, 2022

Язык: Английский

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“Structure”-function relationships in eukaryotic transcription factors: The role of intrinsically disordered regions in gene regulation

Molecular Cell, Год журнала: 2022, Номер 82(21), С. 3970 - 3984

Опубликована: Окт. 19, 2022

Язык: Английский

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A disordered region controls cBAF activity via condensation and partner recruitment

Cell, Год журнала: 2023, Номер 186(22), С. 4936 - 4955.e26

Опубликована: Окт. 1, 2023

Intrinsically disordered regions (IDRs) represent a large percentage of overall nuclear protein content. The prevailing dogma is that IDRs engage in non-specific interactions because they are poorly constrained by evolutionary selection. Here, we demonstrate condensate formation and heterotypic distinct separable features an IDR within the ARID1A/B subunits mSWI/SNF chromatin remodeler, cBAF, establish "sequence grammars" underlying each contribution. Condensation driven uniformly distributed tyrosine residues, partner mediated non-random blocks rich alanine, glycine, glutamine residues. These concentrate specific cBAF protein-protein interaction network essential for localization activity. Importantly, human disease-associated perturbations ARID1B sequence grammars disrupt function cells. Together, these data identify contributions to remodeling explain how phase separation provides mechanism through which both genomic functional recruitment achieved.

Язык: Английский

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Membrane prewetting by condensates promotes tight-junction belt formation

Nature, Год журнала: 2024, Номер 632(8025), С. 647 - 655

Опубликована: Авг. 7, 2024

Biomolecular condensates enable cell compartmentalization by acting as membraneless organelles

Язык: Английский

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Molecular determinants of condensate composition

Molecular Cell, Год журнала: 2025, Номер 85(2), С. 290 - 308

Опубликована: Янв. 1, 2025

Язык: Английский

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The intertwined physics of active chemical reactions and phase separation

Current Opinion in Colloid & Interface Science, Год журнала: 2022, Номер 61, С. 101606 - 101606

Опубликована: Авг. 2, 2022

Язык: Английский

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