A conceptual framework for understanding phase separation and addressing open questions and challenges

Molecular Cell, Journal Year: 2022, Volume and Issue: 82(12), P. 2201 - 2214

Published: June 1, 2022

Language: Английский

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Phase Transitions of Associative Biomacromolecules

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(14), P. 8945 - 8987

Published: March 7, 2023

Multivalent proteins and nucleic acids, collectively referred to as multivalent associative biomacromolecules, provide the driving forces for formation compositional regulation of biomolecular condensates. Here, we review key concepts phase transitions aqueous solutions specifically that include folded domains intrinsically disordered regions. The these systems come under rubric coupled segregative transitions. underlying processes are presented, their relevance condensates is discussed.

Language: Английский

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The Mediator complex as a master regulator of transcription by RNA polymerase II

Nature Reviews Molecular Cell Biology, Journal Year: 2022, Volume and Issue: 23(11), P. 732 - 749

Published: June 20, 2022

Language: Английский

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Capillary forces generated by biomolecular condensates

Nature, Journal Year: 2022, Volume and Issue: 609(7926), P. 255 - 264

Published: Sept. 7, 2022

Language: Английский

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Transcription activation is enhanced by multivalent interactions independent of phase separation

Molecular Cell, Journal Year: 2022, Volume and Issue: 82(10), P. 1878 - 1893.e10

Published: May 1, 2022

Language: Английский

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“Structure”-function relationships in eukaryotic transcription factors: The role of intrinsically disordered regions in gene regulation

Molecular Cell, Journal Year: 2022, Volume and Issue: 82(21), P. 3970 - 3984

Published: Oct. 19, 2022

Language: Английский

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A disordered region controls cBAF activity via condensation and partner recruitment

Cell, Journal Year: 2023, Volume and Issue: 186(22), P. 4936 - 4955.e26

Published: Oct. 1, 2023

Intrinsically disordered regions (IDRs) represent a large percentage of overall nuclear protein content. The prevailing dogma is that IDRs engage in non-specific interactions because they are poorly constrained by evolutionary selection. Here, we demonstrate condensate formation and heterotypic distinct separable features an IDR within the ARID1A/B subunits mSWI/SNF chromatin remodeler, cBAF, establish "sequence grammars" underlying each contribution. Condensation driven uniformly distributed tyrosine residues, partner mediated non-random blocks rich alanine, glycine, glutamine residues. These concentrate specific cBAF protein-protein interaction network essential for localization activity. Importantly, human disease-associated perturbations ARID1B sequence grammars disrupt function cells. Together, these data identify contributions to remodeling explain how phase separation provides mechanism through which both genomic functional recruitment achieved.

Language: Английский

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Membrane prewetting by condensates promotes tight-junction belt formation

Nature, Journal Year: 2024, Volume and Issue: 632(8025), P. 647 - 655

Published: Aug. 7, 2024

Biomolecular condensates enable cell compartmentalization by acting as membraneless organelles

Language: Английский

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Molecular determinants of condensate composition

Molecular Cell, Journal Year: 2025, Volume and Issue: 85(2), P. 290 - 308

Published: Jan. 1, 2025

Language: Английский

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The intertwined physics of active chemical reactions and phase separation

Current Opinion in Colloid & Interface Science, Journal Year: 2022, Volume and Issue: 61, P. 101606 - 101606

Published: Aug. 2, 2022

Language: Английский

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