Nucleic Acids Research,
Год журнала:
2023,
Номер
51(12), С. 6264 - 6285
Опубликована: Май 16, 2023
Abstract
Photodynamic
therapy
(PDT)
ideally
relies
on
the
administration,
selective
accumulation
and
photoactivation
of
a
photosensitizer
(PS)
into
diseased
tissues.
In
this
context,
we
report
new
heavy-atom-free
fluorescent
G-quadruplex
(G4)
DNA-binding
PS,
named
DBI.
We
reveal
by
fluorescence
microscopy
that
DBI
preferentially
localizes
in
intraluminal
vesicles
(ILVs),
precursors
exosomes,
which
are
key
components
cancer
cell
proliferation.
Moreover,
purified
exosomal
DNA
was
recognized
G4-specific
antibody,
thus
highlighting
presence
such
G4-forming
sequences
vesicles.
Despite
absence
signal
from
nuclei,
light-irradiated
DBI-treated
cells
generated
reactive
oxygen
species
(ROS),
triggering
3-fold
increase
nuclear
G4
foci,
slowing
fork
progression
elevated
levels
both
base
damage,
8-oxoguanine,
double-stranded
breaks.
Consequently,
found
to
exert
significant
phototoxic
effects
(at
nanomolar
scale)
toward
lines
tumor
organoids.
Furthermore,
vivo
testing
reveals
induces
not
only
formation
damage
but
also
apoptosis
zebrafish,
specifically
area
where
had
accumulated.
Collectively,
approach
shows
promise
for
image-guided
PDT.
Advanced Healthcare Materials,
Год журнала:
2023,
Номер
12(17)
Опубликована: Март 3, 2023
Abstract
Dressing
change
is
a
significant
and
inevitable
process
during
wound
healing.
Possible
secondary
damage
caused
through
dressing
removal
may
impose
great
threat
on
recovery,
thus
resulting
in
healing
delays
ultimately
higher
cost
of
hospitalization.
Hence,
non‐contact
refreshable
with
an
ease
operation
desire,
especially
for
chronic
wounds
where
long‐term
repeated
would
be
performed.
Herein,
all‐light‐operated
hydrogel
that
achieve
fast
remote‐controllable
(30
s
gelation/4
min
dissolution
upon
light
irradiation)
presented.
In
diabetic
murine
model,
substantially
improved
within
2–3
weeks
observed
due
to
attenuated
changes.
Moreover,
promising
facilitation
the
processes
epithelialization,
collagen
deposition,
cell
proliferation,
inflammatory
regulation
also
detected,
representing
synergistic
effect
photo‐responsive
therapeutic
efficiency.
Proceedings of the National Academy of Sciences,
Год журнала:
2023,
Номер
120(27)
Опубликована: Июнь 26, 2023
The
design
of
stimuli-responsive
systems
in
nanomedicine
arises
from
the
challenges
associated
with
unsolved
needs
current
molecular
drug
delivery.
Here,
we
present
a
delivery
system
high
spatiotemporal
control
and
tunable
release
profiles.
is
based
on
combination
an
hydrophobic
synthetic
rotary
motor
PDMS-b-PMOXA
diblock
copolymer
to
create
responsive
self-assembled
system.
successful
incorporation
selective
activation
by
low-power
visible
light
(λ
=
430
nm,
6.9
mW)
allowed
trigger
fluorescent
dye
efficiencies
(up
75%).
Moreover,
proved
ability
turn
off
behavior
demand
over
sequential
cycles.
Low
concentrations
photoresponsive
units
(down
1
mol%
motor)
are
shown
effectively
promote
release.
Our
was
also
tested
under
relevant
physiological
conditions
using
lung
cancer
cell
line
encapsulation
Food
Drug
Administration
(FDA)-approved
drug.
Similar
levels
viability
observed
compared
free
given
showing
potential
our
platform
deliver
functional
drugs
request
efficiency.
This
work
provides
important
step
for
application
machines
next
generation
smart
systems.
Journal of the American Chemical Society,
Год журнала:
2023,
Номер
145(7), С. 4026 - 4034
Опубликована: Фев. 8, 2023
Herein,
we
present
high-yielding,
concise
access
to
a
set
of
xanthenium-derived,
water-soluble,
low-molecular-weight
photocages
allowing
light-controlled
cargo
release
in
the
green
red
region.
Very
importantly,
these
new
allow
installation
various
payloads
through
ester,
carbamate,
or
carbonate
linkages
even
at
last
stage
synthesis.
Payloads
were
uncaged
with
high
efficiency
upon
green,
orange,
light
irradiation,
leading
carboxylic
acids,
phenols,
and
amines.
The
near-ideal
properties
carboxanthenium
derivative
further
evaluated
context
drug
using
camptothecin-derived
chemotherapeutic
drug,
SN38.
Notably,
caged
showed
orders
magnitude
lower
cellulo,
which
was
reinstated
after
irradiation.
presented
offer
that
facilitate
translation
photoactivated
chemotherapy
toward
clinical
applications.
Advanced Materials,
Год журнала:
2023,
Номер
35(24)
Опубликована: Март 20, 2023
Tumor
vascular
disrupting
therapy
has
offered
promising
opportunities
to
treat
cancer
in
clinical
practice,
whereas
the
overall
therapeutic
efficacy
is
notably
limited
due
off-target
effects
and
repeated
dose
toxicity
of
agents
(VDAs).
To
tackle
this
problem,
a
VDA-free
biomimetic
semiconducting
polymer
nanoparticle
(SPNP
)
herein
reported
for
precise
tumor
disruption
through
two-stage
light
manipulation.
SPNP
consists
as
photothermal
agent
camouflaged
with
platelet
membranes
that
specifically
target
disrupted
vasculature.
Upon
first
photoirradiation,
administered
vivo
generates
mild
hyperthermia
trigger
hemorrhage,
which
activates
coagulation
cascade
recruits
more
injured
blood
vessels.
Such
enhanced
targeting
enables
intense
destroy
vasculature
during
second
leading
complete
eradication
efficient
metastasis
inhibition.
Intriguingly,
mechanism
study
reveals
strategy
alleviates
splenomegaly
reverses
immunosuppressive
microenvironment
by
reducing
myeloid-derived
suppressor
cells.
Therefore,
not
only
illustrates
light-driven
self-recruitment
enhance
via
single
therapeutics
but
also
deciphers
immunotherapeutic
role
conducive
studies.
Nucleic Acids Research,
Год журнала:
2023,
Номер
51(12), С. 6264 - 6285
Опубликована: Май 16, 2023
Abstract
Photodynamic
therapy
(PDT)
ideally
relies
on
the
administration,
selective
accumulation
and
photoactivation
of
a
photosensitizer
(PS)
into
diseased
tissues.
In
this
context,
we
report
new
heavy-atom-free
fluorescent
G-quadruplex
(G4)
DNA-binding
PS,
named
DBI.
We
reveal
by
fluorescence
microscopy
that
DBI
preferentially
localizes
in
intraluminal
vesicles
(ILVs),
precursors
exosomes,
which
are
key
components
cancer
cell
proliferation.
Moreover,
purified
exosomal
DNA
was
recognized
G4-specific
antibody,
thus
highlighting
presence
such
G4-forming
sequences
vesicles.
Despite
absence
signal
from
nuclei,
light-irradiated
DBI-treated
cells
generated
reactive
oxygen
species
(ROS),
triggering
3-fold
increase
nuclear
G4
foci,
slowing
fork
progression
elevated
levels
both
base
damage,
8-oxoguanine,
double-stranded
breaks.
Consequently,
found
to
exert
significant
phototoxic
effects
(at
nanomolar
scale)
toward
lines
tumor
organoids.
Furthermore,
vivo
testing
reveals
induces
not
only
formation
damage
but
also
apoptosis
zebrafish,
specifically
area
where
had
accumulated.
Collectively,
approach
shows
promise
for
image-guided
PDT.
