Curdione combined with borneol treats bacterial mixed HPV infection by regulating the crosstalk among immune cells

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Янв. 22, 2025

Background Human papillomavirus (HPV) infection is a worldwide reproductive system disease. Baofukang suppository, traditional herbal preparation that includes curdione and borneol, has been reported to treat bacterial vaginosis (BV) HPV in China. However, the therapeutic mechanism still unknown. This study aims explore molecular mechanisms of borneol treating infection. Methods We conducted retrospective cohort analysis medical records from single-center involving 205 patients, focusing on correlation between clearance co-infection with other pathogens, confirming efficacy suppository. Bioinformatics network pharmacology approaches were employed identify targets suppository for BV/HPV co-infections. qRT-PCR, Western blot, immunofluorescence staining, flow cytometry utilized validate along associated immune changes. Finally, confirmed vivo using an LPS/TC-1 cervical orthotopic injection model. Results Curdione selectively inhibit secretion interleukin-6 (IL-6) interleukin-1β (IL-1β) by macrophages. The reduction IL-6 IL-1β levels effectively inhibits expression CD274 (Programmed death ligand 1, PD-L1) infected epithelial cells inhibiting STAT3 phosphorylation, thereby suppressing their evasion capabilities. Furthermore, enhance tumor necrosis factor α (TNF-α) caspase 1 (CASP1) macrophages, as well interleukin 12 (IL-12) 23 (IL-23) dendritic (DCs). these inflammatory factors promotes migration differentiation T site infection, completing cells. Conclusion main components progression occurrence cancer modulating communication innate adaptive immunity, promoting recruitment recognition CD8 + eliminate HPV-infected

Язык: Английский

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Interconnectedness threat: unveiling the mechanisms behind human papillomavirus-induced cervical cancer

Exploration of Medicine, Год журнала: 2025, Номер 6

Опубликована: Март 4, 2025

Cervical cancer is the fourth leading cause of cancer-related deaths among women worldwide, causing over 660,000 new cases and 350,000 in 2022, with a disproportionately high burden low-resource countries where access to treatment limited. Human papillomavirus (HPV) common sexually transmitted infection that accounts for approximately 95% cervical cases. Persistent HPV can progress dysplasia, categorized into varying severities (CIN1, CIN2, CIN3), which significantly increases risk. The mechanism HPV-induced malignancy involves disruption cellular apoptosis by integrating viral genetic material cells, particularly within transformation zone. proteins E6 E7 play pivotal roles carcinogenesis inhibiting tumor suppressor proteins, promoting uncontrolled cell proliferation, evading immune responses, ultimately driving progression toward malignancy. Timely detection intervention are essential managing HPV-related cancers. Preventative measures such as vaccination have demonstrated substantial efficacy. Six vaccines targeting high-risk (HR) strains recommended before sexual activity or exposure. Despite these advancements, barriers, misinformation, logistical challenges, limited healthcare infrastructure, persist, underserved regions. Advances diagnostic therapeutic technologies offered avenues addressing challenges. Next-generation sequencing CRISPR gene editing emerging promising tools enable precise targeted interventions. Furthermore, artificial intelligence (AI) imaging innovations enhanced accuracy personalized care. Pap smears DNA testing indispensable early detection. To tackle globally, multifaceted approach required. Public health education, programs, research, international collaboration crucial. campaigns should combat strengthen focus on novel therapies, screening technologies, next-generation sequencing.

Язык: Английский

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A community-focused cervical and breast cancer screening program using a sustainable funding model in a training center in Ghana

BMC Health Services Research, Год журнала: 2025, Номер 25(1)

Опубликована: Март 25, 2025

While Ghana prepares to roll out a nationwide breast and cervical (pre)cancer screening policy, it is necessary continuously document high-impact scalable models. Over the years, Cervical Cancer Prevention Training Centre (CCPTC), Battor, has utilized sustainable funding model in which each trainee pays for 15 women be screened with visual inspection acetic acid. This paper details framework of community-focused trainer-led coordinated outreaches carried under this model. The further reports outcomes over 5-year period discusses advantages shortcomings an effort make recommendations development scale-up combined largely opportunistic setting. descriptive retrospective cross-sectional study investigated who underwent precancer using acid or mobile colposcopy and/or high-risk human papillomavirus (hr-HPV) DNA testing between September 2017 July 2022 (n = 2,273) clinical examination June 2021 March 2023 622) by trainees CCPTC on conducted primarily solidify their practical skills. For HPV inspection, respectively, explored factors associated infection 'positivity' nominal logistic regression. overall prevalence hr-HPV was 14.3% (95% CI, 10.0–19.6) among valid results testing, while rate 2.8% 2.2–3.6). After controlling age, earning income only factor (aOR 3.00; 95% 1.35 − 6.64; p-value 0.007). Factors after adjusting age were: number births 0.71; 0.52 0.97; 0.029), lifetime pregnancies 0.79; 0.67 0.93; 0.004), being single 2.42; 1.19 4.90; 0.014), 0.44; 0.26 0.74; 0.002). Breast showed clinically significant masses 20 (3.2%), lymphadenopathy 13 (2.1%), nipple discharge 37 (6.0%) n 3/67 (4.5%) requiring referral followed up management. outreach approach adopted myriad benefits, evidence-based studies structured program evaluations are needed assess if can large scale, especially without backing training institution resources capacity investigate manage screen positives.

Язык: Английский

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The Burden of HPV Infections and HPV‐Related Diseases Among People With HIV: A Systematic Literature Review

Journal of Medical Virology, Год журнала: 2025, Номер 97(4)

Опубликована: Апрель 1, 2025

Human papillomavirus (HPV) is associated with a significant global burden of precancerous lesions and cancer. People HIV (PWH) are at higher risk HPV infection HPV-related diseases. This systematic review was conducted to synthesize data on the diseases among PWH. Studies published between January 2018-June 2023 were sourced from databases conferences. Included 221 publications containing epidemiological infections clinical The varied by geographical region, age, sex, sexual orientation. Compared people without (PWoH), PWH had prevalence incidence Among PWH, anal ranged 44% 83%; men anogenital warts than women. over two-fold greater transgender women who have sex their respective counterparts HIV. Incident cancer up PWoH, incident cervical six times those without. most prevalent high-risk (hr) genotypes disease vaccine genotype HPV16/18/52/58. HPV35 one or African descent. also concurrent study calls for strengthening appropriate delivery increasing uptake this group, potentially integrating vaccination routine care.

Язык: Английский

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Immunogenic cell death genes in single-cell and transcriptome analyses perspectives from a prognostic model of cervical cancer

Frontiers in Genetics, Год журнала: 2025, Номер 16

Опубликована: Апрель 7, 2025

The role of immunogenic cell death (ICD) in cervical cancer (CESC) is not well understood. This study sought to investigate the significance ICD CESC and establish an ICDRs prognostic model improve immunotherapy efficacy for patients with cancer. ICD-associated genes were screened at single-cell transcriptome levels based on AddModuleScore, single-sample gene set enrichment analysis (ssGSEA) weighted co-expression network (WGCNA) analysis. Immunogenic death-related features (ICDRs) constructed using multiple machine algorithms, evaluated training validation sets provide quantitative tools predicting prognosis clinical practice. Predictive models used risk subgroups response immunotherapy, as drug sensitivity. Finally, expression ICD-related was verified by RT-qPCR. Through integrated data, transcriptomic profiling, computational modeling, seven identified highly patients. Multivariate demonstrated that low-risk had significantly better overall survival compared high-risk patients, confirming independent tool. Assessments tumor microenvironment (TME), mutation characteristics, sensitivity within indicated a stronger group.

Язык: Английский

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The Prevalence of Multi-Type Infections Among Human Papillomavirus Types in Korean Women

Pathogens, Год журнала: 2025, Номер 14(4), С. 369 - 369

Опубликована: Апрель 9, 2025

The distribution of human papillomavirus (HPV) genotypes shows inconsistencies across countries, ethnicities, and socioeconomic levels. An in-depth identification HPV infection rates regions, age groups in large populations is crucial. We aimed to assess the prevalence infections among Korean women investigate multi-type types. To identify types, 16,669 specimens were subjected DNA extraction real-time polymerase chain reactions. rate was 36.7%, with single- 21.4% 15.3%, respectively. higher their 20s 60s. types 16 18 most commonly infected type 52. In conclusion, promoting awareness prevention strategies incentivizing vaccination can boost eligible individuals.

Язык: Английский

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Prevalence and Genotype Distribution of High-Risk HPV Genotypes Among Women in Greece: A Retrospective Analysis of 3500 Women

Cancers, Год журнала: 2025, Номер 17(8), С. 1267 - 1267

Опубликована: Апрель 9, 2025

Background/Objectives: Persistent infection with high-risk (HR) HPV genotypes is the main risk factor for development of cervical cancer. The present analysis provides recent trends on HR-HPV rates and distribution among 3500 Greek women between 2021 2023. Methods: detection HR-HPVs specific identification HPV16 HPV18 were conducted using Roche Cobas 4800 assay. genotyping 12 other was performed through Nested Multiplex PCR methodology (NMPCR). Results: overall rate 8.8% most prevalent genotype being followed by HPV31, HPV66, HPV56, HPV51, HPV58, HPV45, HPV18, HPV68, HPV59, HPV52, HPV35, HPV39, HPV33. Among HR-HPV-positive cases prevalence single, double, triple, quadruple infections 73.9%, 19.9%, 5.5%, 0.7%, respectively. Age-specific showed that higher in age group 31–35 years (25.5%) it estimated multiple occur more often younger women. Notably, varies different groups. It proposed HPV16, HPV66 may show an increased possibility establishing long-term over 36 old. Conclusions: high which are not included prophylactic vaccines underlines significance constant surveillance circulating HPVs population.

Язык: Английский

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Cancer chemoprevention: signaling pathways and strategic approaches

Signal Transduction and Targeted Therapy, Год журнала: 2025, Номер 10(1)

Опубликована: Апрель 17, 2025

Abstract Although cancer chemopreventive agents have been confirmed to effectively protect high-risk populations from invasion or recurrence, only over ten drugs approved by the U.S. Food and Drug Administration. Therefore, screening potent is crucial reduce constantly increasing incidence mortality rate of cancer. Considering lengthy prevention process, an ideal agent should be nontoxic, inexpensive, oral. Natural compounds become a natural treasure reservoir for chemoprevention because their superior ease availability, cost-effectiveness, safety. The benefits as in various studies. In light this, present review intended fully delineate entire scope chemoprevention, primarily focuses on aspects based compounds, specifically focusing mechanism action prevention, discussing detail how they exert effects affecting classical signaling pathways, immune checkpoints, gut microbiome. We also introduce novel strategies summarize role improving chemotherapy regimens. Furthermore, we describe discovering anticancer with low abundance high activity, revealing broad prospects drug discovery chemoprevention. Moreover, associate precision medicine, discuss challenges encountered Finally, emphasize transformative potential advancing field ability more effective less toxic preventive options oncology.

Язык: Английский

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Patterns of Single and Multiple HPV Infections in Female: A Systematic Review and Meta-analysis

Heliyon, Год журнала: 2024, Номер 10(17), С. e35736 - e35736

Опубликована: Авг. 18, 2024

Data on the patterns of single and multiple HPV infections are largely limited to small size studies, regional difference have not been systematically examined.

Язык: Английский

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Protective Mechanisms of Vaginal Lactobacilli against Sexually Transmitted Viral Infections

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(17), С. 9168 - 9168

Опубликована: Авг. 23, 2024

The healthy cervicovaginal microbiota is dominated by various Lactobacillus species, which support a condition of eubiosis. Among their many functions, vaginal lactobacilli contribute to the maintenance an acidic pH, produce antimicrobial compounds, and modulate host immune response protect against bacterial fungal infections. Increasing evidence suggests that these beneficial bacteria may also confer protection sexually transmitted infections (STIs) caused viruses such as human papillomavirus (HPV), immunodeficiency virus (HIV) herpes simplex (HSV). Viral STIs pose substantial public health burden globally, causing range infectious diseases with potentially severe consequences. Understanding molecular mechanisms exert protective effects viral paramount for development novel preventive therapeutic strategies. This review aims provide more recent insights into intricate interactions between STIs, exploring impact on microenvironment, response, infectivity pathogenesis, highlighting potential implications interventions clinical management

Язык: Английский

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