Active Forms of Chemerin Are Elevated in Human and Mouse Ovarian Carcinoma DOI Creative Commons
Lei Zhao, Qin Zhou, Venkatesh Krishnan

и другие.

Biomedicines, Год журнала: 2025, Номер 13(4), С. 991 - 991

Опубликована: Апрель 18, 2025

Background: Chemerin is a small adipokine that activated and inactivated by proteolysis of its C-terminus with role in regulating metabolism, immunity, inflammation. Significant levels chemerin are found circulation ascitic fluid ovarian carcinoma patients. Methods: We investigated the different forms three cohorts: people BMI < 25, > 40, ascites ELISAs specific for forms. Ascites from mouse model were also analyzed, was compared between wild-type chemerin-deficient mice. Results: Conversion plasma to serum increased processed lower increases samples 25 than 40. High total both patients who had mean 29 model. In mice tumors grew slower Conclusions: Serum has more active irrespective source. contained high chemerin, which, based on data, enhance tumor growth.

Язык: Английский

GPCR drug discovery: new agents, targets and indications DOI
José A. Lorente, Aleksandr V. Sokolov, Gavin Ferguson

и другие.

Nature Reviews Drug Discovery, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Язык: Английский

Процитировано

2
Solid‐state NMR protocols for unveiling dynamics and (drug) interactions of membrane‐bound proteins DOI Creative Commons
Alessia Lasorsa, Patrick C.A. van der Wel

Protein Science, Год журнала: 2025, Номер 34(4)

Опубликована: Март 18, 2025

Abstract Magic angle spinning solid‐state NMR (MAS ssNMR) is a versatile tool for studying the structure and dynamics of membrane proteins, as well their interactions with ligands drugs. Its power lies in ability to provide atomic‐level information on samples under physiological‐like conditions. Moreover, it can illuminate across wide range timescales great relevance protein function dysfunction. In this protocol paper, we highlight key aspects sample preparation, data acquisition, interpretation, based our own experience broader literature. We discuss steps along important considerations preparation parameters ssNMR measurements, reference special requirements membrane‐based samples. Such display physiologically relevant different motional regimes that be probed by but also interfere certain measurements. guide reader through whole process from complex characterization techniques. Throughout report, refer back examples prior work between cytochrome c cardiolipin‐containing membranes, discussion lipid dependence peroxidase‐activity inhibitor. conclude short alternative new methods are further boosting versatility study membrane‐bound proteins or drug interactions.

Язык: Английский

Процитировано

0
Active Forms of Chemerin Are Elevated in Human and Mouse Ovarian Carcinoma DOI Creative Commons
Lei Zhao, Qin Zhou, Venkatesh Krishnan

и другие.

Biomedicines, Год журнала: 2025, Номер 13(4), С. 991 - 991

Опубликована: Апрель 18, 2025

Background: Chemerin is a small adipokine that activated and inactivated by proteolysis of its C-terminus with role in regulating metabolism, immunity, inflammation. Significant levels chemerin are found circulation ascitic fluid ovarian carcinoma patients. Methods: We investigated the different forms three cohorts: people BMI < 25, > 40, ascites ELISAs specific for forms. Ascites from mouse model were also analyzed, was compared between wild-type chemerin-deficient mice. Results: Conversion plasma to serum increased processed lower increases samples 25 than 40. High total both patients who had mean 29 model. In mice tumors grew slower Conclusions: Serum has more active irrespective source. contained high chemerin, which, based on data, enhance tumor growth.

Язык: Английский

Процитировано

0